Citation: ZHU Yun-Cheng, WANG Er-Qiong, MA Guo-Lin, KANG Yan-Biao, ZHAO Lin-Hong, LIU Yang-Zhong. Interaction of C-Terminal Metal-Binding Domain of Copper Transport Protein with Ag⁺ and Hg²⁺[J]. Acta Physico-Chimica Sinica, ;2014, 30(1): 1-7. doi: 10.3866/PKU.WHXB201311263 shu

Interaction of C-Terminal Metal-Binding Domain of Copper Transport Protein with Ag⁺ and Hg²⁺

1.
Collaborative Innovation Center of Suzhou Nano Science and Technology, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, P. R. China

Received Date: 4 November 2013
Available Online: 26 November 2013
Fund Project: 国家自然科学基金(U1332210，21171156) (U1332210，21171156)博士后基金(2013M541831)资助项目 (2013M541831)

Copper transport protein (CTR1), which is essential for copper uptake, also plays an important role in the cellular uptake of other heavy metal ions. In this work, the interactions of the C-terminal metalbinding domain of human CTR1 (C8) with both Ag⁺ and Hg²⁺ were studied, using ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry (MS). The results showed that Ag⁺ and Hg²⁺ bind to C8 by different binding modes. Each C8 binds to two Ag⁺, whereas one Hg²⁺ crosslinks two C8 units. In addition, the coordination of Ag⁺ to C8 has an intermediate exchange rate, whereas the binding of Hg²⁺ to C8 has a fast exchange rate. The cysteine residue of C8 is one of the most important binding sites for both Ag⁺ and Hg²⁺. However, histidine residues are also involved in the metalbinding process. Ag⁺ binds histidine preferentially, whereas Hg²⁺ prefers to bind to cysteine residues. Although the HCH motif of C8 is crucial for metal binding, some other residues can also participate in the binding of Ag⁺. These residues may be involved in the metal-transfer process in the cellular uptake of Ag⁺ by CTR1. These results provide important information for a better understanding of the mechanism of cellular uptake of metal ions by CTR1.
- Copper transport protein,
- Ag⁺,
- Hg²⁺,
- Binding mode,
- Nuclear magnetic resonance,
- Mass spectrometry
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Interaction of C-Terminal Metal-Binding Domain of Copper Transport Protein with Ag+ and Hg2+

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通讯作者: 陈斌, bchen63@163.com

Interaction of C-Terminal Metal-Binding Domain of Copper Transport Protein with Ag⁺ and Hg²⁺