Citation: LIU Yuan, LONG Mei, XIE Meng-Xia. Mechanism of Interaction between Chrysin and Different Configurations of Human Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2013, 29(12): 2647-2654. doi: 10.3866/PKU.WHXB201310311 shu

Mechanism of Interaction between Chrysin and Different Configurations of Human Serum Albumin

1.
1 Analytical & Testing Center of Beijing Normal University, Beijing 100875, P. R. China;
2 Department of Chemistry and Chemical Technology, Chifeng University, Chifeng 024000, Inner Mon lia Autonomous Region, P. R. China

Received Date: 28 June 2013
Available Online: 31 October 2013
Fund Project: 内蒙古自治区自然科学基金(2012MS0212)资助项目 (2012MS0212)

The mechanism of the interaction between Chrysin (CHR) and human serum albumin (HSA) was investigated using various spectroscopic techniques. It was found that CHR can induce HSA fluorescence emission quenching by static process. Additionally, HSA caused a significant red shift in the ultraviolet absorption of CHR. This indicated that binding with a protein can result in dissociation of the phenolic hydroxyl in CHR. HSA can also cause CHR fluorescence emission. The binding constants of CHR and HAS were calculated based on the fluorescence quenching and emission modes. The results obtained using these two methods were consistent. The binding constant (K_A) values at pH 7.4 were (9.97±0.24)×10⁴ and (9.75±0.11)×10⁴ L·mol^-1, respectively; and the ratio of combination was 1:1. The binding constants decreased gradually with decreasing the pH value. This is related to conformational changes in the protein. The protein is partly denatured at pH 3.5, with the site Ⅱ in the HSA opening chain. It was found that CHR was located at site I, which is in subdomain ⅡA of HAS. Based on molecular modeling, the mechanism of the interaction between CHR and HAS was investigated.
- Chrysin,
- Human serum albumin,
- Fluorescence,
- pH effect,
- Molecular modeling
1. [1]
  
  (1) Soares, S.; Mateus, N.; Freitas, V. D. J. Agric. Food Chem.2007, 55, 6726. doi: 10.1021/jf070905x
2. [2]
  (2) Yang, Y. M.; Hu, Q. L.; Fan, Y. L.; Shen, H. S. SpectrochimicaActa Part A 2008, 69, 432. doi: 10.1016/j.saa.2007.04.019
3. [3]
  (3) Li, D. J.; Zhu, J. F.; Jin, J.; Yao, X. J. Journal of MolecularStructure 2007, 846, 34. doi: 10.1016/j.molstruc.2007.01.020
4. [4]
  (4) Kanakias, C. D.; Nafisi, S.; Rajai, M.; Shadaloi, A.; Tarantilis, P.A.; Polissiou, M. J.; Bariyanga, J.; Tajmir-Riahi, H. A.Spectroscopy 2009, 23, 29. doi: 10.1155/2009/154321
5. [5]
  (5) Ma, J.; Liu, Y.; Xie, M. X. Spectroscopy and Spectral Analysis2012, 32 (1), 1. [马纪, 刘媛, 谢孟峡. 光谱学与光谱分析, 2012, 32 (1), 1.]
6. [6]
  (6) Ma, G. Z.; Tan, F.; Jiang, Y. J.; Zheng, K.W.; Guo, M.; Yu, Q.C. Acta Phys. -Chim. Sin. 2005, 21 (2), 123. [马国正, 谭非,蒋勇军, 郑柯文, 郭明, 俞庆森. 物理化学学报, 2005, 21 (2),123.] doi: 10.3866/PKU.WHXB20050203
7. [7]
  (7) Guo, Q. L.; Li, R.; Jiang, F. L.; Tu, J. C.; Li, L.W.; Liu, Y. ActaPhys. -Chim. Sin. 2009, 25 (10), 2147. [郭清莲, 李冉, 蒋风雷, 涂建成, 李林尉, 刘义. 物理化学学报, 2009, 25 (10),2147.] doi: 10.3866/PKU.WHXB20091112
8. [8]
  (8) Twine, S. M.; re, M. G.; Morton, P. Arch. Biochem. Biophys.2003, 414, 83. doi: 10.1016/S0003-9861(03)00173-5
9. [9]
  (9) Yamasaki, K.; Maruyama, T.; Yoshimoto, K.; Tsutsumi, Y.Biochim. Biophys. Acta 1999, 1432, 313. doi: 10.1016/S0167-4838(99)00098-9
10. [10]
  (10) Ahmad, B.; Ankita; Khan, R. H. Arch. Biochem. Biophys. 2005,437, 159. doi: 10.1016/j.abb.2005.03.013
11. [11]
  (11) Sakai, T.; Yamasaki, K.; Sako, T.; Kragh, H. U.; Suenaga, A.;Otagiri, M. Pharm. Res. 2001, 18 (4), 520. doi: 10.1023/A:1011014629551
12. [12]
  (12) Zanoli, P.; Avalloneb, R.; Baraldi, M. Fitoterapia 2000, 71,117. doi: 10.1016/S0367-326X(00)00186-6
13. [13]
  (13) Castro, G. T.; Ferretti, F. H.; Blanco, S. E. Spectrochimica ActaPart A 2005, 62, 657. doi: 10.1016/j.saa.2005.02.013
14. [14]
  (14) Friedman, M.; Jurgens, H. S. J. Agric. Food Chem. 2000, 48,2101. doi: 10.1021/jf990489j
15. [15]
  (15) Tommasini, S.; Calabro, M. L.; Donato, P. J. Pharm. Biomed.Anal. 2004, 35, 389. doi: 10.1016/S0731-7085(03)00586-7
16. [16]
  (16) Qin, C.; Xie, M. X.; Liu, Y. Biomacromolecules 2007, 8,2182. doi: 10.1021/bm070319c
17. [17]
  (17) Liu, Y.; Xie, M. X.; Jiang, M.;Wang, Y. D. SpectrochimicaActa Part A 2005, 61, 2245. doi: 10.1016/j.saa.2004.09.004
18. [18]
  (18) Rasoulzadeh, F.; Jabary, H. N.; Naseri, A.; Rashidi, M. R.Spectrochimica Acta Part A 2009, 72, 190. doi: 10.1016/j.saa.2008.09.021
19. [19]
  (19) Yu, Z. L.; Li, D. J.; Ji, B. M.; Chen, J. J. Journal of MolecularStructure 2008, 889, 422. doi: 10.1016/j.molstruc.2008.03.013
20. [20]
  (20) Papadopoulou, A.; Green, R. J.; Frazier, R. A. J. Agric. FoodChem. 2005, 53, 158. doi: 10.1021/jf048693g
21. [21]
  (21) Maurice, R.; Eftink; Camillo, A.; Ghiron. Analyt. Biochem.1981, 114, 199. doi: 10.1016/0003-2697(81)90474-7
22. [22]
  (22) Lakowicz, J. R. Principles of Fluorescence Spectroscopy;Plenum Press: New York, 1983; p 257.
23. [23]
  (23) Fang, R.; Leng, X. J.;Wu, X.; Li, Q.; Hao, R. F.; Ren, F. Z.;Jing, H. Spectroscopy and Spectral Analysis 2012, 32 (1), 108.[方茹, 冷小京, 吴夏, 李琦, 郝瑞芳, 任发政, 景浩.光谱学与光谱分析, 2012, 32 (1), 108.]
24. [24]
  (24) Bhattacharya, J.; Bhattacharya, M.; Chakrabarty, A. S.;Chaudhury, U.; Poddar, R. K. Biochem. Pharmacology 1994,47, 2049. doi: 10.1016/0006-2952(94)90080-9
25. [25]
  (25) Sytnik, A.; Lritvinyuk, I. Proceedings of the National Academyof Sciences of the United States of America 1996, 93 (23),12959. doi: 10.1073/pnas.93.23.12959
1. [1]
  YanYuan Jia , Rong Rong , Jie Liu , Jing Guo , GuoYu Jiang , Shuo Guo . Unity is Strength, and Independence Shines: A Science Popularization Experiment on AIE and ACQ Effects. University Chemistry, 2024, 39(9): 349-358. doi: 10.12461/PKU.DXHX202402035
2. [2]
  Qin Li , Kexin Yang , Qinglin Yang , Xiangjin Zhu , Xiaole Han , Tao Huang . Illuminating Chlorophyll: Innovative Chemistry Popularization Experiment. University Chemistry, 2024, 39(9): 359-368. doi: 10.3866/PKU.DXHX202309059
3. [3]
  Feng Zheng , Ruxun Yuan , Xiaogang Wang . “Research-Oriented” Comprehensive Experimental Design in Polymer Chemistry: the Case of Polyimide Aerogels. University Chemistry, 2024, 39(10): 210-218. doi: 10.12461/PKU.DXHX202404027
4. [4]
  Shuwen SUN , Gaofeng WANG . Two cadmium coordination polymers constructed by varying Ⅴ-shaped co-ligands: Syntheses, structures, and fluorescence properties. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 613-620. doi: 10.11862/CJIC.20230368
5. [5]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
6. [6]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
7. [7]
  Kexin Dong , Chuqi Shen , Ruyu Yan , Yanping Liu , Chunqiang Zhuang , Shijie Li . Integration of Plasmonic Effect and S-Scheme Heterojunction into Ag/Ag₃PO₄/C₃N₅ Photocatalyst for Boosted Photocatalytic Levofloxacin Degradation. Acta Physico-Chimica Sinica, 2024, 40(10): 2310013-. doi: 10.3866/PKU.WHXB202310013
8. [8]
  Yan ZHAO , Xiaokang JIANG , Zhonghui LI , Jiaxu WANG , Hengwei ZHOU , Hai GUO . Preparation and fluorescence properties of Eu³⁺-doped CaLaGaO₄ red-emitting phosphors. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1861-1868. doi: 10.11862/CJIC.20240242
9. [9]
  Xinyu Liu , Weiran Hu , Zhengkai Li , Wei Ji , Xiao Ni . Algin Lab: Surging Luminescent Sea. University Chemistry, 2024, 39(5): 396-404. doi: 10.3866/PKU.DXHX202312021
10. [10]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084
11. [11]
  Pingping Zhu , Yongjun Xie , Yuanping Yi , Yu Huang , Qiang Zhou , Shiyan Xiao , Haiyang Yang , Pingsheng He . Excavation and Extraction of Ideological and Political Elements for the Virtual Simulation Experiments at Molecular Level: Taking the Project “the Simulation and Computation of Conformation, Morphology and Dimensions of Polymer Chains” as an Example. University Chemistry, 2024, 39(2): 83-88. doi: 10.3866/PKU.DXHX202309063
12. [12]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
13. [13]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
14. [14]
  Jianjun Liu , Xue Yang , Chi Zhang , Xueyu Zhao , Zhiwei Zhang , Yongmei Chen , Qinghong Xu , Shao Jin . Preparation and Fluorescence Characterization of CdTe Semiconductor Quantum Dots. University Chemistry, 2024, 39(7): 307-315. doi: 10.3866/PKU.DXHX202311031
15. [15]
  Zishuo Yi , Peng Liu , Yan Xu . Fluorescent “Chameleon”: A Popular Science Experiment Based on Dynamic Luminescence. University Chemistry, 2024, 39(9): 304-310. doi: 10.12461/PKU.DXHX202311079
16. [16]
  Yixuan Zhu , Qingtong Wang , Jin Li , Lin Chen , Junlong Zhao . Blog of Oxytocin. University Chemistry, 2024, 39(9): 134-140. doi: 10.12461/PKU.DXHX202310090
17. [17]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
18. [18]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
19. [19]
  Lin Song , Dourong Wang , Biao Zhang . Innovative Experimental Design and Research on Preparing Flexible Perovskite Fluorescent Gels Using 3D Printing. University Chemistry, 2024, 39(7): 337-344. doi: 10.3866/PKU.DXHX202310107
20. [20]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357

Get Citation

PDF

XML

Metrics

PDF Downloads(516)
Abstract views(804)
HTML views(24)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142