Citation: NING Ai-Min, MENG Lei, ZHAO Zhong-Lin, ZHENG Xian-Fu, WAN Xin-Sheng. Mechanism of Interaction between Bovine Serum Albumin and Sodium Dodecyl Sulfate[J]. Acta Physico-Chimica Sinica, ;2013, 29(12): 2639-2646. doi: 10.3866/PKU.WHXB201310281 shu

Mechanism of Interaction between Bovine Serum Albumin and Sodium Dodecyl Sulfate

1.
College of Science, Henan Agricultural University, Zhengzhou 450002, P. R. China

Received Date: 15 July 2013
Available Online: 28 October 2013
Fund Project: 国家自然科学基金(31100067)资助项目 (31100067)

The binding isotherms of the interaction between bovine serum albumin (BSA) and sodium dodecyl sulfate (SDS) were obtained using electromotive force measurements. Changes in the microenvironmental polarity of aromatic amino acid residues during the interaction were studied using fourth-derivative ultraviolet spectroscopy and fluorescence spectroscopy. The average number (v) of SDS molecules bound to BSA increased with increasing SDS concentration. The polarity of tryptophan (Trp) residues decreased gradually and then remained almost constant. The polarity of tyrosine residues increased significantly and then decreased a little. The polarity of phenylalanine residues increased very slightly. The results show that SDS molecules bind to BSA in the vicinity of Trp-213 when v gradually increases from 0 to 14. BSA unfolds from domain ⅡA, induced by SDS aggregates formed near Trp-213. The v value then increases rapidly as a result of positive cooperative binding. When the v value reaches about 302, saturation binding is achieved and the BSA conformation remains almost unchanged.
- Electromotive force,
- Fourth-derivative ultraviolet spectroscopy,
- Fluorescence spectroscopy,
- Aromatic amino acid residue,
- Microenvironment,
- Polarity,
- Binding site
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