Citation: YANG Da-Wei, CHEN Chao, XIE Qing-Ji, YAO Shou-Zhuo. Comparison of Enzymatic Activities and Electroactivities of Adsorbed Glucose Oxidase on Several Nanomaterial-Modified Electrodes[J]. Acta Physico-Chimica Sinica, ;2013, 29(08): 1727-1734. doi: 10.3866/PKU.WHXB201306042 shu

Comparison of Enzymatic Activities and Electroactivities of Adsorbed Glucose Oxidase on Several Nanomaterial-Modified Electrodes

1.
College of Chemistry and Chemical Engineering, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Changsha 410081, P. R. China

Received Date: 23 March 2013
Available Online: 4 June 2013
Fund Project: 国家自然科学基金(21175042, 21075036) (21175042, 21075036)湖南省教育厅(11A069) (11A069)

The quartz-crystal microbalance technique was used to monitor the adsorption of glucose oxidase ( x) on bare Au, Au-electrodeposited Au (Au_ed/Au), multiwalled carbon nanotube (MWCNT)- modified Au (MWCNTs/Au), and Au-electrodeposited MWCNT-modified Au (Au_ed/MWCNTs/Au) electrodes. The mass of x at saturated adsorption was obtained in each case. The amperometric responses of these enzyme electrodes were examined, and the mass-specific bioactivities of the immobilized (adsorbed) x (MSBA_i) were evaluated through anodic potentiostatic detection of enzymatically generated H₂O₂ in the presence of glucose. The direct electrochemistry of adsorbed x was studied by cyclic voltammetry, to obtain the electroactivity percentage of the immobilized (adsorbed) x (EAP_i) in each case. We found that the amounts of enzyme adsorbed and the amperometric responses of these enzyme electrodes follow the order MWCNTs/Au > Au_ed/MWCNTs/Au > Au_ed/Au > Au; MSBA_i follows the order of Au > Au_ed/MWCNTs/Au > Au_ed/Au > MWCNTs/Au; and EAP_i follows the order of MWCNTs/Au > Au_ed/MWCNTs/Au > Au_ed/Au > Au. The experimental data are interpreted based on hydrophobic/hydrophilic interactions among enzyme molecules and the nanomaterials, as well as the amount of adsorbed x. It was also verified that the total enzymatic activity of all the electrode-adsorbed enzyme molecules is positively related to the amperometric responses of the enzyme electrodes. This work is useful in studying the immobilization of enzyme on nanomaterials and thus-prepared amperometric enzyme electrodes.
- Quartz crystal microbalance,
- Glucose oxidase,
- Mass-specific bioactivity,
- Electroactivity percentage,
- Multiwalled carbon nanotubes,
- Electrodeposition of Au nanoparticles
1. [1]
  
  (1) Wang, J. Electroanalysis 2001, 13, 983.
2. [2]
  (2) Ianniello, R. M.; Yacynych, A. M. Anal. Chem. 1981, 53, 2090.doi: 10.1021/ac00236a033
3. [3]
  (3) Chen, C.; Xie, Q. J.; Yang, D.W.; Xiao, H. L.; Fu, Y. C.; Tan, Y.M.; Yao, S. Z. RSC Adv. 2013, 3, 4473. doi: 10.1039/c2ra22351a
4. [4]
  (4) Wang, J. Chem. Rev. 2008, 108, 815.
5. [5]
  (5) Alex, H. H. T.; Fabio, L.; Simone, R. S.; Gilberto, M. J. Phys. Chem. C 2012, 116, 18857. doi: 10.1021/jp305633v
6. [6]
  (6) Yang, C.; Xu, C. X.;Wang, X. M. Langmuir 2012, 28, 4580.doi: 10.1021/la2044202
7. [7]
  (7) Tellechea, E.;Wilson, K. J.; Bravo, E.; Schifferli, K. H.Langmuir 2012, 28, 5190. doi: 10.1021/la2050866
8. [8]
  (8) Jeong, B.; Akter, R.; Han, O. H.; Rhee, C. K.; Rahman, M. A.Anal. Chem. 2013, 85, 1784. doi: 10.1021/ac303142e
9. [9]
  (9) ran, J. M.; Mantilla, S. M.; Stevenson, K. J. Anal. Chem.2013, 85, 1571. doi: 10.1021/ac3028036
10. [10]
  (10) Wang, T.; Fu, Y. C.; Bu, L. J.; Qin, C.; Meng, Y.; Chen, C.; Ma,M.; Xie, Q. J.; Yao, S. Z. J. Phys. Chem. C. 2012, 116, 20908.doi: 10.1021/jp306492a
11. [11]
  (11) Alwarappan, S.; Boyapalle, S.; Kumar, A.; Li, C. Z.; Mohaparea,S. S. J. Phys. Chem. C 2012, 116, 6556. doi: 10.1021/jp211201b
12. [12]
  (12) Xie, Q. J.;Wang, J.; Zhou, A.; Zhang, Y. Y.; Liu, H.; Xu, Z.;Yuan, Y.; Deng, M.; Yao, S. Z. Anal. Chem. 1999, 71, 4649.doi: 10.1021/ac981390z
13. [13]
  (13) Xie, Q. J.; Xiang, C.; Yuan, Y.; Zhang, Y.; Nie, L.; Yao, S. Z.J. Colloid Interface Sci. 2003, 262, 107. doi: 10.1016/S0021-9797(03)00196-6
14. [14]
  (14) Marx, K. A. Biomacromolecules 2003, 4, 1099. doi: 10.1021/bm020116i
15. [15]
  (15) Buttry, D. A.;Ward, M. D. Chem. Rev. 1992, 92, 1355.doi: 10.1021/cr00014a006
16. [16]
  (16) Sauerbrey, G. Z. Phys. 1959, 155, 206. doi: 10.1007/BF01337937
17. [17]
  (17) Kanazawa, K. K.; rdon, J. G. Anal. Chem. Acta 1985, 175,99. doi: 10.1016/S0003-2670(00)82721-X
18. [18]
  (18) Chen, X.; Zhang, Q. L.; Su, Y. H.; Meng,W. H.; Xie, Q. J.; Yao,S. Z. Acta Phys. -Chim. Sin. 2007, 23, 1201. [陈昕, 张漪丽,苏育华, 孟文华, 谢青季, 姚守拙. 物理化学学报, 2007, 23,1201.] doi: 10.3866/PKU.WHXB20070812
19. [19]
  (19) Xie, Q. J.; Zhang, Y. Y.; Xu, M. C.; Yao, S. Z. J. Electroanal. Chem. 1999, 478, 1.
20. [20]
  (20) Fu, Y. C.; Li, P. H.; Bu, L. J.;Wang, T.; Xie, Q. J.; Chen, J. H.;Yao, S. Z. Anal. Chem. 2011, 83, 6511. doi: 10.1021/ac200471v
1. [1]
  Xiufang Wang , Donglin Zhao , Kehua Zhang , Xiaojie Song . “Preparation of Carbon Nanotube/SnS₂ Photoanode Materials”: A Comprehensive University Chemistry Experiment. University Chemistry, 2024, 39(4): 157-162. doi: 10.3866/PKU.DXHX202308025
2. [2]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
3. [3]
  Hailang JIA , Hongcheng LI , Pengcheng JI , Yang TENG , Mingyun GUAN . Preparation and performance of N-doped carbon nanotubes composite Co₃O₄ as oxygen reduction reaction electrocatalysts. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 693-700. doi: 10.11862/CJIC.20230402
4. [4]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
5. [5]
  Haihua Yang , Minjie Zhou , Binhong He , Wenyuan Xu , Bing Chen , Enxiang Liang . Synthesis and Electrocatalytic Performance of Iron Phosphide@Carbon Nanotubes as Cathode Material for Zinc-Air Battery: a Comprehensive Undergraduate Chemical Experiment. University Chemistry, 2024, 39(10): 426-432. doi: 10.12461/PKU.DXHX202405100
6. [6]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
7. [7]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357
8. [8]
  Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101
9. [9]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
10. [10]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
11. [11]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
12. [12]
  Yu Wang , Shoulei Zhang , Tianming Lv , Yan Su , Xianyu Liu , Fuping Tian , Changgong Meng . Introduce a Comprehensive Inorganic Synthesis Experiment: Synthesis of Nano Zinc Oxide via Microemulsion Using Waste Soybean Oil. University Chemistry, 2024, 39(7): 316-321. doi: 10.3866/PKU.DXHX202311035
13. [13]
  Shicheng Yan . Experimental Teaching Design for the Integration of Scientific Research and Teaching: A Case Study on Organic Electrooxidation. University Chemistry, 2024, 39(11): 350-358. doi: 10.12461/PKU.DXHX202408036
14. [14]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
15. [15]
  Qi Li , Pingan Li , Zetong Liu , Jiahui Zhang , Hao Zhang , Weilai Yu , Xianluo Hu . Fabricating Micro/Nanostructured Separators and Electrode Materials by Coaxial Electrospinning for Lithium-Ion Batteries: From Fundamentals to Applications. Acta Physico-Chimica Sinica, 2024, 40(10): 2311030-. doi: 10.3866/PKU.WHXB202311030
16. [16]
  Xiaomei Ning , Liang Zhan , Xiaosong Zhou , Jin Luo , Xunfu Zhou , Cuifen Luo . Preparation and Electro-Oxidation Performance of PtBi Supported on Carbon Cloth: A Recommended Comprehensive Chemical Experiment. University Chemistry, 2024, 39(11): 217-224. doi: 10.3866/PKU.DXHX202401085
17. [17]
  Xinting XIONG , Zhiqiang XIONG , Panlei XIAO , Xuliang NIE , Xiuying SONG , Xiuguang YI . Synthesis, crystal structures, Hirshfeld surface analysis, and antifungal activity of two complexes Na(Ⅰ)/Cd(Ⅱ) assembled by 5-bromo-2-hydroxybenzoic acid ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1661-1670. doi: 10.11862/CJIC.20240145
18. [18]
  Lei Shi . Nucleophilicity and Electrophilicity of Radicals. University Chemistry, 2024, 39(11): 131-135. doi: 10.3866/PKU.DXHX202402018
19. [19]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
20. [20]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036

Get Citation

PDF

XML

Metrics

PDF Downloads(774)
Abstract views(677)
HTML views(24)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142