Citation: YU Zhan-Jiang, CHEN Yong-Qiang, YANG Xiao-Da. Effective Adsorption of Functional Biological Macromolecules on Stainless Steel Surface with Micro/Nanoporous Texture[J]. Acta Physico-Chimica Sinica, ;2013, 29(07): 1595-1602. doi: 10.3866/PKU.WHXB201305082 shu

Effective Adsorption of Functional Biological Macromolecules on Stainless Steel Surface with Micro/Nanoporous Texture

1.
1 State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, P. R. China;
2 Lepu Medical Technology (Beijing) Co., Ltd., Beijing 100022, P. R. China

Received Date: 4 March 2013
Available Online: 8 May 2013
Fund Project: 国家自然科学基金(30770581, 20971008) (30770581, 20971008)高等学校博士学科点专项科研基金(20090001110068)资助项目 (20090001110068)

Stainless steel (AISI 316L) is commonly used as a material in medical devices. Modification of the metal surface with bioactive molecules and/or nanoparticles to develop next-generation smart biomaterial, e.g., cardiovascular stents, has recently attracted great attention. The present work investigated adsorption of antibodies and enzymes on micro/nanoporous 316L stainless steel compared with that on smooth and ld-coated stainless steel surfaces. The experimental results showed that the micro/nanoporous stainless steel surface produced by electrochemical erosion can adsorb a large amount of proteins (antibodies or horse radish peroxidase (HRP)), with comparable or better results than the sputtered- ld surface. Washes with surfactants (e.g., 10% bull serum albumin (BSA) or 0.2% Tween 20 solution) did not remove the enzymes/antibodies. In contrast, pretreatment of the metal plates with 5% Tween 20 halved antibody adsorption but did not affect adsorption of HRP. The wettability of the porous metal surface coated with proteins depended on the protein species and amount of protein adsorbed. The protein-coated porous surface was hydrophilic (water contact angle<50°), which should make it biocompatible. The proteins on the micro/nanoporous stainless steel surface retained their high biological activity; in particular, micro/nanoporous stainless steel stents modified with an anti-CD34 antibody using the present method can effectively and selectively capture KG-1 cells. Our work provides a basis for developing novel polymer-free, smart, economic biomaterials with stainless steel for biomedical applications.
- Micro/nano texture,
- Stainless steel,
- Antibody,
- Horse radish peroxidase,
- Surface adsorption
1. [1]
  
  (1) Holzapfel, B. M.; Reichert, J. C.; Schantz, J. T.; Gbureck, U.;Rackwitz, L.; Noth, U.; Jakob, F.; Rudert, M.; Groll, J.;Hutmacher, D.W. Adv. Drug Deliv. Rev. 2013, 65, 581. doi: 10.1016/j.addr.2012.07.009
2. [2]
  (2) Nagarajan, S.; Mohana, M.; Sudhagar, P.; Raman, V.;Nishimura, T.; Kim, S.; Kang, Y. S.; Rajendran, N. ACS Appl. Mater. Interfaces 2012, 4, 5134.
3. [3]
  (3) Abdel-Fattah, T. M.; Loftis, D.; Mahapatro, A. J. Biomed. Nanotechnol. 2011, 7, 794. doi: 10.1166/jbn.2011.1346
4. [4]
  (4) Hayes, J. S.; Richards, R. G. Expert. Rev. Med. Devices 2010, 7,843. doi: 10.1586/erd.10.53
5. [5]
  (5) Weckbach, S.; Losacco, J. T.; Hahnhaussen, J.; Gebhard, F.;Stahel, P. F. Unfallchirurg 2012, 115, 75. doi: 10.1007/s00113-011-2145-0
6. [6]
  (6) Joung, Y. K.; You, S. S.; Park, K. M.; , D. H.; Park, K. D.Colloids Surf. B: Biointerfaces 2012, 99, 102. doi: 10.1016/j.colsurfb.2011.10.047
7. [7]
  (7) Slaney, A. M.;Wright, V. A.; Meloncelli, P. J.; Harris, K. D.;West, L. J.; Lowary, T. L.; Buriak, J. M. ACS Appl. Mater. Interfaces 2011, 3, 1601. doi: 10.1021/am200158y
8. [8]
  (8) Lionetto, S.; Little, A.; Moriceau, G.; Heymann, D.; Decurtins,M.; Plecko, M.; Filgueira, L.; Cadosch, D. J. Biomed. Mater. Res. A 2013, 101, 991.
9. [9]
  (9) Yang, Z.; Tu, Q.; Zhu, Y.; Luo, R.; Li, X.; Xie, Y.; Maitz, M. F.;Wang, J.; Huang, N. Adv. Healthc. Mater. 2012, 1, 548. doi: 10.1002/adhm.201200073
10. [10]
  (10) Kang, C. K.; Lim,W. H.; Kyeong, S.; Choe,W. S.; Kim, H. S.;Jun, B. H.; Lee, Y. S. Colloids Surf. B: Biointerfaces 2013, 102,744. doi: 10.1016/j.colsurfb.2012.09.008
11. [11]
  (11) Caro, A.; Humblot, V.; Methivier, C.; Minier, M.; Salmain, M.;Pradier, C. M. J. Phys. Chem. B 2009, 113, 2101. doi: 10.1021/jp805284s
12. [12]
  (12) Kang, C. K.; Lee, Y. S. J. Mater. Sci. Mater. Med. 2007, 18,1389. doi: 10.1007/s10856-006-0079-9
13. [13]
  (13) Ceylan, H.; Tekinay, A. B.; Guler, M. O. Biomaterials 2011, 32,8797. doi: 10.1016/j.biomaterials.2011.08.018
14. [14]
  (14) Davis, E. M.; Li, D. Y.; Irvin, R. T. Biomaterials 2011, 32, 5311.doi: 10.1016/j.biomaterials.2011.04.027
15. [15]
  (15) Ignatova, M.; Voccia, S.; Gabriel, S.; Gilbert, B.; Cossement,D.; Jerome, R.; Jerome, C. Langmuir 2009, 25, 891. doi: 10.1021/la802472e
16. [16]
  (16) Imamura, K.; Kawasaki, Y.; Awadzu, T.; Sakiyama, T.;Nakanishi, K. J. Colloid Interface Sci. 2003, 267, 294. doi: 10.1016/S0021-9797(03)00700-8
17. [17]
  (17) Falentin-Daudre, C.; Faure, E.; Svaldo-Lanero, T.; Farina, F.;Jerome, C.; Van DeWeerdt, C.; Martial, J.; Duwez, A. S.;Detrembleur, C. Langmuir 2012, 28, 7233. doi: 10.1021/la3003965
18. [18]
  (18) Harvey, J.; Bergdahl, A.; Dadafarin, H.; Ling, L.; Davis, E. C.;Omanovic, S. Biotechnol. Lett. 2012, 34, 1159. doi: 10.1007/s10529-012-0885-8
19. [19]
  (19) Secker, T. J.; Herve, R.; Zhao, Q.; Borisenko, K. B.; Abel, E.W.; Keevil, C.W. Biofouling 2012, 28, 563. doi: 10.1080/08927014.2012.698387
20. [20]
  (20) Horia, N.; Iwasaa, F.; Uenoa, T.; Takeuchib, K.; Tsukimuraa, N.;Yamadaa, M.; Hattorib, M.; Yamamotoc, A.; Ogawaa, T. Dental Materials 2010, 26, 275. doi: 10.1016/j.dental.2009.11.077
21. [21]
  (21) Subramanian, B.; Ananthakumar, R.; Kobayashi, A.;Jayachandran, M. J. Mater. Sci. Mater. Med. 2012, 23, 329. doi: 10.1007/s10856-011-4500-7
22. [22]
  (22) Subramanian, B.; Dhandapani, P.; Maruthamuthu, S.;Jayachandran, M. J. Biomater. Appl. 2012, 26, 687. doi: 10.1177/0885328210377534
23. [23]
  (23) Valanezahad, A.; Ishikawa, K.; Tsuru, K.; Maruta, M.; Matsuya,S. Dent. Mater. J. 2011, 30, 749. doi: 10.4012/dmj.2010-153
24. [24]
  (24) Buhagiar, J.; Bell, T.; Sammons, R.; Dong, H. J. Mater. Sci. Mater. Med. 2011, 22, 1269.
25. [25]
  (25) Wendel, H. P.; Avci-Adali, M.; Ziemer, G. Int. J. Cardiol. 2010,145, 115. doi: 10.1016/j.ijcard.2009.06.020
26. [26]
  (26) Granada, J. F.; Inami, S.; Aboodi, M. S.; Tellez, A.; Milewski,K.;Wallace-Bradley, D.; Parker, S.; Rowland, S.; Nakazawa,G.; Vorpahl, M.; Kolodgie, F. D.; Kaluza, G. L.; Leon, M. B.;Virmani, R. Circ. Cardiovasc. Interv. 2010, 3, 257. doi: 10.1161/CIRCINTERVENTIONS.109.919936
27. [27]
  (27) McGuigan, A. P.; Sefton, M. V. Biomaterials 2007, 28, 2547.doi: 10.1016/j.biomaterials.2007.01.039
28. [28]
  (28) Rossi, M. L.; Zavalloni, D.; Gasparini, G. L.; Man , R.; Belli,G.; Presbitero, P. Int. J. Cardiol. 2010, 141, e20.
29. [29]
  (29) Le Guehennec, L.; Martin, F.; Lopez-Heredia, M. A.; Louarn,G.; Amouriq, Y.; Cousty, J.; Layrolle, P. Nanomedicine 2008, 3,61. doi: 10.2217/17435889.3.1.61
30. [30]
  (30) Pan, H. A.; Liang, J. Y.; Hung, Y. C.; Lee, C. H.; Chiou, J. C.;Huang, G. S. Biomaterials 2013, 34, 841. doi: 10.1016/j.biomaterials.2012.09.078
31. [31]
  (31) Ranellaa, A.; Barberogloua, M.; Bakogiannia, S.; Fotakisa, C.;Stratakisa, E. Acta Biomaterialia 2010, 6, 2711. doi: 10.1016/j.actbio.2010.01.016
32. [32]
  (32) Nayak, B. K.; Gupta, M. C. Optics and Lasers in Engineering2010, 48, 940. doi: 10.1016/j.optlaseng.2010.04.010
33. [33]
  (33) Fukuzaki, S.; Urano, H.; Nagata, K. J. Ferment. Bioeng. 1995,80, 6. doi: 10.1016/0922-338X(95)98168-K
34. [34]
  (34) Bee, J. S.; Chiu, D.; Sawicki, S.; Stevenson, J. L.; Chatterjee,K.; Freund, E.; Carpenter, J. F.; Randolph, T.W. J. Pharm. Sci.2009, 98, 3218.
35. [35]
  (35) Sakiyama, T.; Aya, A.; Embutsu, M.; Imamura, K.; Nakanishi,K. J. Biosci. Bioeng. 2006, 101, 434. doi: 10.1263/jbb.101.434
36. [36]
  (36) Hagiwara, T.; Sakiyama, T.;Watanabe, H. Langmuir 2009, 25,226.
37. [37]
  (37) He, C. X.; Yuan, A. P.; Zhang, Q. L.; Ren, X. Z.; Li, C. H.; Liu,J. H. Acta Phys. -Chim. Sin. 2012, 28, 2721. [何传新, 袁安朋,张黔玲, 任祥忠, 李翠华, 刘剑洪. 物理化学学报, 2012, 28,2721.] doi: 10.3866/PKU.WHXB201207191
38. [38]
  (38) Zhang, F.; Guo,W.; Yu, Z.;Wang, Y. C. Chin. J. Pharm. Anal.2011, 31, 862.
39. [39]
  (39) Berry, J. L.; Santamarina, A.; Moore, J. E., Jr.; Roychowdhury,S.; Routh,W. D. Ann. Biomed. Eng. 2000, 28, 386. doi: 10.1114/1.276
40. [40]
  (40) Hao, L.; Lawrence, J. Proc. Inst. Mech. Eng. H 2006, 220, 47.doi: 10.1243/095441105X68999
41. [41]
  (41) Mikulewicz, M.; Chojnacka, K. Biol. Trace. Elem. Res. 2011,142, 865. doi: 10.1007/s12011-010-8798-7
42. [42]
  (42) Matsumura, H.; Saburi, M. Colloids Surf. B: Biointerfaces 2006,47, 146. doi: 10.1016/j.colsurfb.2005.12.004
43. [43]
  (43) Mourtas, S.; Kastellorizios, M.; Klepetsanis, P.; Farsari, E.;Amanatides, E.; Mataras, D.; Pistillo, B. R.; Favia, P.; Sardella,E.; d'A stino, R.; Antimisiaris, S. G. Colloids Surf. B: Biointerfaces 2011, 84, 214. doi: 10.1016/j.colsurfb.2011.01.002
44. [44]
  (44) Muller, R.; Abke, J.; Schnell, E.; Macionczyk, F.; Gbureck, U.;Mehrl, R.; Ruszczak, Z.; Kujat, R.; Englert, C.; Nerlich, M.;Angele, P. Biomaterials 2005, 26, 6962. doi: 10.1016/j.biomaterials.2005.05.013
45. [45]
  (45) Liu, P.; Xing, G.W.; Li, X.W.; Ye, Y. H. Acta Phys. -Chim. Sin.2010, 26, 1113. [刘平, 邢国文, 李宣文, 叶蕴华. 物理化学学报, 2010, 26, 1113.] doi: 10.3866/PKU.WHXB20100448
46. [46]
  (46) Omanovic, S.; Roscoe, S. G. J. Colloid Interface Sci. 2000, 227,452. doi: 10.1006/jcis.2000.6913
47. [47]
  (47) Bee, J. S.; Davis, M.; Freund, E.; Carpenter, J. F.; Randolph, T.W. Biotechnol. Bioeng. 2010, 105, 121. doi: 10.1002/bit.v105:1
48. [48]
  (48) Hedberg, Y. S.; Killian, M. S.; Blomberg, E.; Virtanen, S.;Schmuki, P.; OdnevallWallinder, I. Langmuir 2012, 28, 16306.doi: 10.1021/la3039279
49. [49]
  (49) Desroches, M. J.; Omanovic, S. Phys. Chem. Chem. Phys. 2008,10, 2502. doi: 10.1039/b719371h
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