Citation: LIU Yan-Cheng, LI Hai-Xia, CUI Ron-Grong, XU Yu-Lie, WANG Wen-Feng. Phosphate Base Effect on DNA Damage Photo-Induced by Ciprofloxacin[J]. Acta Physico-Chimica Sinica, ;2013, 29(01): 212-216. doi: 10.3866/PKU.WHXB201211131 shu

Phosphate Base Effect on DNA Damage Photo-Induced by Ciprofloxacin

1.
1 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
2 University of Chinese Academy of Science, Beijing 100049, P. R. China

Received Date: 31 August 2012
Available Online: 13 November 2012
Fund Project: 国家自然科学基金(21173252)资助项目 (21173252)

Stable and transient spectroscopic studies were conducted to investigate the influence of DNA phosphate bases in the process of DNA damage photo-induced by ciprofloxacin (CPX). The results of UV-visible and fluorescence studies confirmed that the absorption and emission properties of CPX were affected by the concentration of phosphate buffer (PB), and these results indicated that there are interactions between CPX and the phosphate anion. To investigate the relationship between the phosphate base and CPX, a comparative experiment was conducted using guanosine (Gua), 2'-deoxyguanosine (dG), and 2'-deoxyguanosine-5'c-monophosphate (dGMP). By comparative experiments, we found that the type of spectrum of CPX triplet state was changed due to the phosphate base of dGMP, hence, we concluded that the photo-damage process was changed. The effects of phosphate base of dGMP on the CPX triplet state (³CPX^*) spectrum have been investigated by laser flash photolysis methods, and the results confirmed that there are hydrogen-bonding interactions between the phosphate base of dGMP and CPX. In this study, we found that CPX and dGMP are combined together by the hydrogen bond which changed the mode of DNA damage photo-induced by CPX. Finally, based on the results obtained in this study, a rational scheme to describe the role of phosphate base playing in the DNA damage process photo-induced by CPX is proposed.
- Photo-induced damage,
- Phosphate base,
- Ciprofloxacin,
- dGMP,
- Laser flash photolysis,
- DNA damage
1. [1]
  
  (1) Domagala, J. M.; Hanna, L. D.; Heifetz, C. L.; Hutt, M. P.;Mich, T. F.; Sanchez, J. P.; Solomon, M. J. Med. Chem. 1986, 29 (3), 394. doi: 10.1021/jm00153a015
2. [2]
  (2) Van Doorslaer, X.; Demeestere, K.; Heynderickx, P. M.; VanLangenhove, H.; Dewulf, J. Appl. Catal. B-Environ. 2011, 101 (3-4), 540. doi: 10.1016/j.apcatb.2010.10.027
3. [3]
  (3) Cuquerella, M. C.; Bosca, F.; Miranda, M. A.; Belvedere, A.;Catalfo, A.; de Guidi, G. Chem. Res. Toxicol. 2003, 16 (4), 562.doi: 10.1021/tx034006o
4. [4]
  (4) Lorenzo, F.; Navaratnam, S.; Edge, R.; Allen, N. S. Photochem. Photobiol. 2008, 84 (5), 1118. doi: 10.1111/php.2008.84.issue-5
5. [5]
  (5) Lorenzo, F.; Navaratnam, S.; Edge, R.; Allen, N. S. Photochem. Photobiol. 2009, 85 (4), 886. doi: 10.1111/php.2009.85.issue-4
6. [6]
  (6) Martinez, L. J.; Sik, R. H.; Chignell, C. F. Photochem. Photobiol. 1998, 67 (4), 399.
7. [7]
  (7) Gurbay, A.; nthier, B.; Signorini-Allibe, N.; Barret, L.;Favier, A.; Hincal, F. Neurotoxicology 2006, 27 (1), 6. doi: 10.1016/j.neuro.2005.05.007
8. [8]
  (8) Belvedere, A.; Bosca, F.; Catalfo, A.; Cuquerella, M.; Guidi, G.;Ma, M. Chem. Res. Toxicol. 2002, 15 (9), 1142. doi: 10.1021/tx025530i
9. [9]
  (9) Hartmann, A.; let, E. M.; Gartiser, S.; Alder, A. C.; Koller, T.;Widmer, R. M. Arch. Environ. Con. Tox. 1999, 36 (2), 115. doi: 10.1007/s002449900449
10. [10]
  (10) Fasani, E.; Negra, F. F. B.; Mella, M.; Monti, S.; Albini, A.J. Org. Chem. 1999, 64 (15), 5388. doi: 10.1021/jo982456t
11. [11]
  (11) Vasconcelos, T. G.; Henriques, D. M.; Konig, A.; Martins, A. F.Chemosphere 2009, 76 (4), 487. doi: 10.1016/j.chemosphere.2009.03.022
12. [12]
  (12) Paul, T.; Dodd, M. C.; Strathmann, T. J. Water Res. 2010, 44 (10), 3121. doi: 10.1016/j.watres.2010.03.002
13. [13]
  (13) Sortino, S.; Condorelli, G. New J. Chem. 2001, 26 (1), 250.
14. [14]
  (14) Pascual-Reguera, M. I.; Parras, G. P.; Diaz, A. M. Microchem. J. 2004, 77 (1), 79. doi: 10.1016/j.microc.2004.01.003
15. [15]
  (15) Shang, Z. C.; Yi, P. G.; Yu, Q. S.; Lin, R. S. Acta Phys. -Chim. Sin. 2001, 17 (1), 48. [商志才, 易平贵, 俞庆森, 林瑞森. 物理化学学报, 2001, 17 (1), 48. ] doi: 10.3866/PKU.WHXB20010110
16. [16]
  (16) Cui, L.; Ren, B.; Tian, Z. Q. Acta Phys. -Chim. Sin. 2010, 26 (2), 397. [崔丽, 任斌, 田中群. 物理化学学报, 2010, 26 (2), 397.] doi: 10.3866/PKU.WHXB20100136
17. [17]
  (17) Mella, M.; Fasani, E.; Albini, A. Helv. Chim. Acta 2001, 84 (9),2508. doi: 10.1002/1522-2675(20010919)84:9<2508::AID-HLCA2508>3.0.CO;2-Y
18. [18]
  (18) Joshi, H. C.; Bridhkoti, J. P.; Mishra, H.; Pant, S. Spectrochim. Acta A 2011, 79 (3), 412. doi: 10.1016/j.saa.2011.02.044
19. [19]
  (19) Albini, A.; Monti, S. Chem. Soc. Rev. 2003, 32 (4), 238. doi: 10.1039/b209220b
20. [20]
  (20) Spratt, T. E.; Schultz, S. S.; Levy, D. E.; Chen, D.; Schluter, G.;Williams, G. M. Chem. Res. Toxicol. 1999, 12 (9), 809.
21. [21]
  (21) Wasielewski, M. R. Chem. Rev. 1992, 92 (3), 435. doi: 10.1021/cr00011a005
22. [22]
  (22) Ferguson, J.; Johnson, B. E. Brit. J. Dermatol. 1990, 123 (1), 9.doi: 10.1111/bjd.1990.123.issue-1
23. [23]
  (23) Monti, S.; Sortino, S.; Fasani, E.; Albini, A. Chem. -Eur. J.2001, 7 (10), 2185.
24. [24]
  (24) Chen, J.W.; Ge, L. K.;Wei, X. X.; Zhang, S. Y.; Qiao, X. L.;Cai, X. Y.; Xie, Q. Environ. Sci. Technol. 2010, 44 (7), 2400.doi: 10.1021/es902852v
25. [25]
  (25) Takacsnovak, K.; Noszal, B.; Hermecz, I.; Kereszturi, G.;Podanyi, B.; Szasz, G. J. Pharm. Sci.-Us. 1990, 79 (11), 1023.
26. [26]
  (26) Liu, Y. C.; Zhang, P.; Li, H. X.;Wang,W. F. Photochem. Photobiol. 2012, 88 (3), 639. doi: 10.1111/php.2012.88.issue-3
1. [1]
  Zizheng LU , Wanyi SU , Qin SHI , Honghui PAN , Chuanqi ZHAO , Chengfeng HUANG , Jinguo PENG . Surface state behavior of W doped BiVO₄ photoanode for ciprofloxacin degradation. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 591-600. doi: 10.11862/CJIC.20230225
2. [2]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
3. [3]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
4. [4]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
5. [5]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
6. [6]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
7. [7]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
8. [8]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
9. [9]
  Zhao Lu , Hu Lv , Qinzhuang Liu , Zhongliao Wang . Modulating NH₂ Lewis Basicity in CTF-NH₂ through Donor-Acceptor Groups for Optimizing Photocatalytic Water Splitting. Acta Physico-Chimica Sinica, 2024, 40(12): 2405005-. doi: 10.3866/PKU.WHXB202405005
10. [10]
  Ronghao Zhao , Yifan Liang , Mengyao Shi , Rongxiu Zhu , Dongju Zhang . Investigation into the Mechanism and Migratory Aptitude of Typical Pinacol Rearrangement Reactions: A Research-Oriented Computational Chemistry Experiment. University Chemistry, 2024, 39(4): 305-313. doi: 10.3866/PKU.DXHX202309101
11. [11]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
12. [12]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
13. [13]
  Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
14. [14]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
15. [15]
  Zhenlin Zhou , Siyuan Chen , Yi Liu , Chengguo Hu , Faqiong Zhao . A New Program of Voltammetry Experiment Teaching Based on Laser-Scribed Graphene Electrode. University Chemistry, 2024, 39(2): 358-370. doi: 10.3866/PKU.DXHX202308049
16. [16]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
17. [17]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
18. [18]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
19. [19]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
20. [20]
  Zijian Zhao , Yanxin Shi , Shicheng Li , Wenhong Ruan , Fang Zhu , Jijun Jiang . A New Exploration of the Preparation of Polyacrylic Acid by Free Radical Polymerization Based on the Concept of Green Chemistry. University Chemistry, 2024, 39(5): 315-324. doi: 10.3866/PKU.DXHX202311094

Get Citation

PDF

XML

Metrics

PDF Downloads(1368)
Abstract views(5065)
HTML views(20)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142