Citation: ZHANG Zhu-Qing. Folding Mechanism of De novo Designed Proteins[J]. Acta Physico-Chimica Sinica, ;2012, 28(10): 2381-2389. doi: 10.3866/PKU.WHXB201209144 shu

Folding Mechanism of De novo Designed Proteins

ZHANG Zhu-Qing

1.
College of Life Science, Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received Date: 24 August 2012
Available Online: 14 September 2012
Fund Project: 中国科学院研究生院院长基金B(Y15102GN00) (Y15102GN00)北京分子科学国家实验室(筹)开放基金(Y15301P1A8)资助 (筹)开放基金(Y15301P1A8)

Protein de novo design and protein folding are two different means to investigate“sequencestructure- function”relationship of proteins, which is one of the most important focuses in structural biology. The successful achievements in protein de novo design indicate the understanding accuracy of the knowledge in protein structure and interaction, while most of those designed proteins show different folding kinetic features from nature occurring proteins, which implies that there are still many challenges to the aim of getting them to play expected biological function. In this review, the status of research and development for protein de novo design, as well as the study progress of protein folding in experimental, theoretical and simulation aspects, have been introduced. Further, the investigations of folding mechanism of de novo designed proteins have been reviewed, and the new clue has been proposed that systematically investigation of the essence of different folding mechanism between the two types of protein would help to provide useful insight for more efficient protein rotational design.
- Protein de novo design,
- Protein folding,
- Target structure,
- Kinetic features of folding
1. [1]
  
  (1) Anfinsen, C. B.; Haber, E.; Sela, M.; White, F. H., Jr. Proc. Natl. Acad. Sci. U. S. A. 1961, 47, 1309. doi: 10.1073/pnas.47.9.1309
2. [2]
  (2) Anfinsen, C. B. Science 1973, 181, 223. doi: 10.1126/science.181.4096.223
3. [3]
  (3) Berman, H. M.;Westbrook, J.; Feng, Z.; Gilliland, G.; Bhat, T.N.;Weissig, H.; Shindyalov, I. N.; Bourne, P. E. Nucleic Acids Res. 2000, 28, 235. doi: 10.1093/nar/28.1.235
4. [4]
  (4) Uversky, V. N. Protein Sci. 2002, 11, 739. doi: 10.1110/ps.4210102
5. [5]
  (5) Huang, Y. Q.; Liu, Z. R. Acta Phys. -Chim. Sin. 2010, 26, 2061.[黄永棋, 刘志荣. 物理化学学报, 2010, 26, 2061.]
6. [6]
  (6) Moult, J.; Pedersen, J. T.; Judson, R.; Fidelis, K. Proteins 1995,23, R2.
7. [7]
  (7) Minton, A. P. Curr. Opin. Struct. Biol. 2000, 10, 34. doi: 10.1016/S0959-440X(99)00045-7
8. [8]
  (8) Jackson, S. E.; Fersht, A. R. Biochemistry 1991, 30, 10428. doi: 10.1021/bi00107a010
9. [9]
  (9) Leopold, P. E.; Montal, M.; Onuchic, J. N. Proc. Natl. Acad. Sci. U. S. A. 1992, 89, 8721. doi: 10.1073/pnas.89.18.8721
10. [10]
  (10) Dill, K. A.; Chan, H. S. Nat. Struct. Biol. 1997, 4, 10. doi: 10.1038/nsb0197-10
11. [11]
  (11) Duan, Y.; Kollman, P. A. Science 1998, 282, 740. doi: 10.1126/science.282.5389.740
12. [12]
  (12) Shaw, D. E.; Maragakis, P.; Lindorff-Larsen, K.; Piana, S.; Dror,R. O.; Eastwood, M. P.; Bank, J. A.; Jumper, J. M.; Salmon, J.K.; Shan, Y.;Wriggers,W. Science 2010, 330, 341. doi: 10.1126/science.1187409
13. [13]
  (13) Lindorff-Larse, K.; Piana, S.; Dror, R. O.; Shaw, D. E. Science2011, 334, 517. doi: 10.1126/science.1208351
14. [14]
  (14) Shortle, D.; Dimaio, D.; Nathans, D. Annu. Rev. Genet. 1981,15, 265. doi: 10.1146/annurev.ge.15.120181.001405
15. [15]
  (15) Leatherbarrow, R. J.; Fersht, A. R. Protein Eng. 1986, 1, 7. doi: 10.1093/protein/1.1.7
16. [16]
  (16) Ho, S. P.; DeGrado,W. F. J. Am. Chem. Soc. 1987, 109, 6751.doi: 10.1021/ja00256a032
17. [17]
  (17) Ramirez-Alvarado, M.; Blanco, F. J.; Serrano, L. Nat. Struct. Biol. 1996, 3, 604. doi: 10.1038/nsb0796-604
18. [18]
  (18) Floudas, C. A. Biotechnol. Bioeng. 2007, 97, 207. doi: 10.1002/bit.21411
19. [19]
  (19) Zhang, Y. Curr. Opin. Struct. Biol. 2008, 18, 342. doi: 10.1016/j.sbi.2008.02.004
20. [20]
  (20) Floudas, C. A.; Fung, H. K.; McAllister, S. R.; Monnigmann,M.; Rajgaria, R. Chem. Eng. Sci. 2006, 61, 966.
21. [21]
  (21) Shakhnovich, E. Chem. Rev. 2006, 106, 1559. doi: 10.1021/cr040425u
22. [22]
  (22) Dill, K. A.; Ozkan, S. B.; Shell, M. S.; Thomas, R.W. Annu. Rev. Biophys. 2008, 37, 289. doi: 10.1146/annurev.biophys.37.092707.153558
23. [23]
  (23) Thirumalai, D.; O'Brien E. P.; Morrison, G.; Hyeon, C. Annu. Rev. Biophys. 2010, 39, 159. doi: 10.1146/annurev-biophys-051309-103835
24. [24]
  (24) Butterfoss, G. L.; Kuhlman, B. Annu. Rev. Biophys. Biomol. Struct. 2006, 35, 49. doi: 10.1146/annurev.biohhys.35.040405.102046
25. [25]
  (25) Pantazes, R. J.; Grisewood, M. J.; Maranas, C. D. Curr. Opin. Struct. Biol. 2011, 21, 467. doi: 10.1016/j.sbi.2011.04.005
26. [26]
  (26) Lai, L. H. Structure Prediction and Molecular Design of Proteins; Peking University Press: Beijing, 1993. [来鲁华.蛋白质的结构预测与分子设计. 北京: 北京大学出版社,1993.]
27. [27]
  (27) Chou, P. Y.; Fasman, G. D. Biochemistry 1974, 13, 222. doi: 10.1021/bi00699a002
28. [28]
  (28) Richardson, J. S.; Richardson, D. C. Science 1988, 240, 1648.doi: 10.1126/science.3381086
29. [29]
  (29) Kumar, S.; Bansal, M. Proteins 1998, 31, 460. doi: 10.1002/(SICI)1097-0134(19980601)31:4<460::AID-PROT12>3.0.CO;2-D
30. [30]
  (30) liaei, B.; Minuchehr, Z. FEBS Lett. 2003, 537, 121. doi: 10.1016/S0014-5793(03)00105-4
31. [31]
  (31) Fonseca, N. A.; Camacho, R.; Magalhaes, A. L. Proteins 2008,70, 188.
32. [32]
  (32) Qi, Y. F.; Liang, H. H.; Han, X. F.; Lai, L. L. Protein Pept. Lett.2012, 19, 345. doi: 10.2174/092986612799363118
33. [33]
  (33) Ho, S. P.; DeGrado,W. F. J. Am. Chem. Soc. 1987, 109, 6751.doi: 10.1021/ja00256a032
34. [34]
  (34) Hecht, M. H.; Richardson, J. S.; Richardson, D. C.; Ogden, R.C. Science 1990, 249, 884. doi: 10.1126/science.2392678
35. [35]
  (35) Fezoui, Y.;Weaver, D. L.; Osterhout, J. J. Proc. Natl. Acad. Sci. U. S. A. 1994, 91, 3675. doi: 10.1073/pnas.91.9.3675
36. [36]
  (36) Bryson, J.W.; Desjarlais, J. R.; Handel, T. M.; DeGrado,W. F.Protein Sci. 1998, 7, 1404. doi: 10.1002/pro.5560070617
37. [37]
  (37) Moser, R.; Thomas, R. M.; Gutte, B. FEBS Lett. 1983, 157,247. doi: 10.1016/0014-5793(83)80555-9
38. [38]
  (38) Blanco, F. J.; Jimenez, M. A.; Herranz, J.; Rico, M.; Santoro, J.;Nieto, J. L. J. Am. Chem. Soc. 1993, 115, 5887. doi: 10.1021/ja00066a092
39. [39]
  (39) Blanco, F. J.; Rivas, G.; Serrano, L. Nat. Struct. Biol. 1994, 1,584. doi: 10.1038/nsb0994-584
40. [40]
  (40) Ramirez-Alvarado, M.; Blanco, F. J.; Serrano, L. Nat. Struct. Biol. 1996, 3, 604. doi: 10.1038/nsb0796-604
41. [41]
  (41) Kortemme, T.; Ramirez-Alvarado, M.; Serrano, L. Science1998, 281, 253. doi: 10.1126/science.281.5374.253
42. [42]
  (42) Carulla, N.;Woodward, C.; Barany, G. Protein. Sci. 2002, 11,1539. doi: 10.1110/ps.4440102
43. [43]
  (43) Dahiyat, B. I.; Mayo, S. L. Science 1997, 278, 82. doi: 10.1126/science.278.5335.82
44. [44]
  (44) Riddle, D. S.; Santia , J. V.; Bray-Hall, S. T.; Doshi, N.;Grantcharova, V. P.; Yi, Q.; Baker, D. Nat. Struct. Biol. 1997, 4,805. doi: 10.1038/nsb1097-805
45. [45]
  (45) Kim, D. E.; Gu, H.; Baker, D. Proc. Natl. Acad. Sci. U. S. A.1998, 95, 4982. doi: 10.1073/pnas.95.9.4982
46. [46]
  (46) Dantas, G.; Kuhlman, B.; Callender, D.;Wong, M.; Baker, D.J. Mol. Biol. 2003, 332, 449. doi: 10.1016/S0022-2836(03)00888-X
47. [47]
  (47) Kuhlman, B.; Dantas, G.; Ireton, G. C.; Varani, G.; Stoddard, B.L.; Baker, D. Science 2003, 302, 1364. doi: 10.1126/science.1089427
48. [48]
  (48) Liang, H.; Chen, H.; Fan, K.;Wei, P.; Guo, X.; Jin, C.; Zeng, C.;Tang, C.; Lai, L. Angew. Chem. Int. Edit. 2009, 48, 3301. doi: 10.1002/anie.200805476
49. [49]
  (49) Zhu, C.; Zhang, C.; Liang, H.; Lai, L. Protein & Cell 2011, 2,1006. doi: 10.1007/s13238-011-1121-3
50. [50]
  (50) Samish, I.; MacDermaid, M.; Perez-Aguilar, J. M.; Saven, J. G.Annu. Rev. Phys. Chem. 2011, 62, 129. doi: 10.1146/annurevphyschem-032210-103509
51. [51]
  (51) Li, H.; Helling, R.; Tang, C.;Wingreen, N. Science 1996, 273,666. doi: 10.1126/science.273.5275.666
52. [52]
  (52) Saven, J. G. Chem. Rev. 2011, 101, 3113.
53. [53]
  (53) Hellinga, H.W. Proc. Natl. Acad. Sci. U. S. A. 1997, 94, 10015.doi: 10.1073/pnas.94.19.10015
54. [54]
  (54) Zhang, C. S.; Lai, L. H. Biochem. Soc. Trans. 2011, 39, 1382.doi: 10.1042/BST0391382
55. [55]
  (55) Zhang, C. S.; Lai, L. H. Proteins 2012, 80, 1078. doi: 10.1002/prot.24009
56. [56]
  (56) Robertson, D. E.; Farid, R. S.; Moser, C. C.; Urbauer, J. L.;Mulholland, S. E.; Pidikiti, R.; Lear, J. D.;Wand, A. J.;DeGrado,W. F.; Dutton, P. L. Nature 1994, 368, 425. doi: 10.1038/368425a0
57. [57]
  (57) Reynolds, K. A.; Hanes, M. S.; Thomson, J. M.; Antczak, A. J.;Berger, J. M.; Bonomo, R. A.; Kirsch, J. F.; Handel, T. M.J. Mol. Biol. 2008, 382, 1265. doi: 10.1016/j.jmb.2008.05.051
58. [58]
  (58) Liu, S.; Zhu, X.; Liang, H.; Cao, A.; Chang, Z.; Lai, L. Proc. Natl. Acad. Sci. U. S. A. 2007, 104, 5330. doi: 10.1073/pnas.0606198104
59. [59]
  (59) Bai, H. J.; Lai, L. H. Acta Phys. -Chim. Sin. 2010, 26, 1988.[白红军, 来鲁华. 物理化学学报, 2010, 26, 1988.] doi: 10.3866/PKU.WHXB20100725
60. [60]
  (60) Jiang, L.; Althoff, E. A.; Clemente, F. R.; Doyle, L.;Rothlisberger, D.; Zanghellini, A.; Gallaher, J. L.; Betker, J. L.;Tanaka, F.; Barbas, C. F., III; Hilvert, D.; Houk, K. N.;Stoddard, B. L.; Baker, D. Science 2008, 319, 1387. doi: 10.1126/science.1152692
61. [61]
  (61) Stefani, M.; Dobson, C. M. J. Mol. Med. 2003, 81, 678. doi: 10.1007/s00109-003-0464-5
62. [62]
  (62) Levinthal, C. J. Chem. Phys. 1968, 65, 44.
63. [63]
  (63) Viguera, A. R.; Martinez, J. C.; Filimonov, V. V.; Mateo, P. L.;Serrano, L. Biochemistry 1994, 33, 2142. doi: 10.1021/bi00174a022
64. [64]
  (64) Otzen, D. E.; Kristensen, O.; Proctor, M.; Oliveberg, M.Biochemistry 1999, 38, 6499. doi: 10.1021/bi982819j
65. [65]
  (65) Plaxco, K.W.; Baker, D. Proc. Natl. Acad. Sci. U. S. A. 1998,95, 13591. doi: 10.1073/pnas.95.23.13591
66. [66]
  (66) Taverna, D. M.; ldstein, R. A. Proteins 2002, 46, 105. doi: 10.1002/prot.10016
67. [67]
  (67) Matouschek, A.; Kellis, J. T.; Serrano, L.; Fersht, A. R. Nature1989, 340, 122. doi: 10.1038/340122a0
68. [68]
  (68) Fersht, A. R.; Matouschek, A.; Serrano, L. J. Mol. Biol. 1992,224, 771. doi: 10.1016/0022-2836(92)90561-W
69. [69]
  (69) Scaloni, F.; Federici, L.; Brunori, M.; Gianni, S. Proc. Natl. Acad. Sci. U. S. A. 2010, 95, 4982.
70. [70]
  (70) Morris, E. R.; Searle, M. S. Curr. Protoc. Protein. Sci. 2012, 28,Unit 28.2.1.
71. [71]
  (71) mez-Hens, A.; Perez-Bendito, D. Anal. Chim. Acta 1991,242, 147. doi: 10.1016/0003-2670(91)87060-K
72. [72]
  (72) Bai, Y.W.; Sosnick, T. R.; Mayne, L.; Englander, S.W. Science1995, 269, 192. doi: 10.1126/science.7618079
73. [73]
  (73) Gai, F.; Du, D.; Xu, Y. Methods. Mol. Biol. 2007, 350, 1.
74. [74]
  (74) Borgia, A.;Williams, P. M.; Clarke, J. Annu. Rev. Biochem.2008, 77, 101. doi: 10.1146/annurev.biochem.77.060706.093102
75. [75]
  (75) Chung, H. S.; McHale, K.; Louis, J. M.; Eaton,W. A. Science2012, 335, 981. doi: 10.1126/science.1215768
76. [76]
  (76) Onuchic, J. N.; Luthey-Schulten, Z.;Wolynes, P. G. Annu. Rev. Phys. Chem. 1997, 48, 545. doi: 10.1146/annurev.physchem.48.1.545
77. [77]
  (77) Gsponer, J.; Vendruscolo, M. Protein Pept. Lett. 2006, 13, 287.doi: 10.2174/092986606775338407
78. [78]
  (78) Dill, K. A. Biochemistry 1985, 24, 1501. doi: 10.1021/bi00327a032
79. [79]
  (79) Clementi, C. Curr. Opin. Struct. Biol. 2008, 18, 10. doi: 10.1016/j.sbi.2007.10.005
80. [80]
  (80) Ueeda, Y.; Taketomi, H.; Gō, N. Biopolymers 1978, 17, 1531.doi: 10.1002/bip.1978.360170612
81. [81]
  (81) Plaxco, K.W.; Simons, K. T.; Baker, D. J. Mol. Biol. 1998, 277,985. doi: 10.1006/jmbi.1998.1645
82. [82]
  (82) Clementi, C.; Nymeyer, H.; Onuchic, J. N. J. Mol. Biol. 2000,298, 937. doi: 10.1006/jmbi.2000.3693
83. [83]
  (83) Hills, R. D., Jr.; Brooks, C. L., III. Int. J. Mol. Sci. 2009, 10,889. doi: 10.3390/ijms10030889
84. [84]
  (84) Chan, H. S.; Zhang, Z.;Wallin, S.; Liu, Z. Annu. Rev. Phys. Chem. 2011, 62, 301. doi: 10.1146/annurev-physchem-032210-103405
85. [85]
  (85) Cho, S. S.; Levy, Y.;Wolynes, P. G. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 434. doi: 10.1073/pnas.0810218105
86. [86]
  (86) Cho, S. S.;Weinkam, P.;Wolynes, P. G. Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 118. doi: 10.1073/pnas.0709376104
87. [87]
  (87) Zuo, G.;Wang, J.;Wang,W. Proteins 2006, 63, 165. doi: 10.1002/prot.20857
88. [88]
  (88) Badasyan, A.; Liu, Z.; Chan, H. S. J. Mol. Biol. 2008, 384, 512.doi: 10.1016/j.jmb.2008.09.023
89. [89]
  (89) Liu, Z.; Chan, H. S. J. Mol. Biol. 2005, 349, 872. doi: 10.1016/j.jmb.2005.03.084
90. [90]
  (90) Ferguson, A.; Liu, Z.; Chan, H. S. J. Mol. Biol. 2009, 389, 619.doi: 10.1016/j.jmb.2009.04.011
91. [91]
  (91) Zarrine-Afsar, A.;Wallin, S.; Neculai, A. M.; Neudecker, P.;Howell, P. L.; Davidson, A. R.; Chan, H. S. Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 9999. doi: 10.1073/pnas.0801874105
92. [92]
  (92) Zarrine-Afsar, A.; Zhang, Z.; Schweiker, K. L.; Makhatadze, G.I.; Davidson, A. R.; Chan, H. S. Proteins 2012, 80, 858. doi: 10.1002/prot.23243
93. [93]
  (93) Su, J. G.; Chen,W. Z.;Wang, C. X. Proteins 2010, 78, 2157.
94. [94]
  (94) Azia, A.; Levy, Y. J. Mol. Biol. 2009, 393, 527. doi: 10.1016/j.jmb.2009.08.010
95. [95]
  (95) Liu, Z.; Reddy, G.; O'Brien, E. P.; Thirumalai, D. Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 7787. doi: 10.1073/pnas.1019500108
96. [96]
  (96) Liu, Z.; Reddy, G.; Thirumalai, D. J. Phys. Chem. B 2012, 116,6707. doi: 10.1021/jp211941b
97. [97]
  (97) Wu, L.; Li,W.; Liu, F.; Zhang, J.;Wang, J.;Wang,W. J. Chem. Phys. 2009, 131, 065105. doi: 10.1063/1.3200952
98. [98]
  (98) Wu, L.; Zhang, J.;Wang, J.; Li,W. F.;Wang,W. Phys. Rev. E2007, 75, 031914. doi: 10.1103/PhysRevE.75.031914
99. [99]
  (99) Wu, L.; Zhang, J.; Qin, M.; Liu, F.;Wang,W. J. Chem. Phys.2008, 128, 235103. doi: 10.1063/1.2943202
100. [100]
  (100) McCammon, J. A.; Gelin, B. R.; Karplus, M. Nature 1977,267, 585. doi: 10.1038/267585a0
101. [101]
  (101) Zhu, Y.; Alonso, D. O.; Maki, K.; Huang, C. Y.; Lahr, S. J.;Daggett, V.; Roder, H.; DeGrado,W. F.; Gai, F. Proc. Natl. Acad. Sci. U. S. A. 2003, 100, 15486. doi: 10.1073/pnas.2136623100
102. [102]
  (102) Zhu, Y.; Fu, X.;Wang, T.; Tamura, A.; Takada, S.; Saven, J. G.;Gai, F. Chem. Phys. 2004, 307, 99. doi: 10.1016/j.chemphys.2004.05.008
103. [103]
  (103) Wang, T.; Zhu, Y.; Gai, F. J. Phys. Chem. B 2004, 108, 3694.doi: 10.1021/jp049652q
104. [104]
  (104) Gillespie, B.; Vu, D. M.; Shah, P. S.; Marshall, S. A.; Dyer, R.B.; Mayo, S. L.; Plaxco, K.W. J. Mol. Biol. 2003, 330, 813.doi: 10.1016/S0022-2836(03)00616-8
105. [105]
  (105) Scalley-Kim, M.; Baker, D. J. Mol. Biol. 2004, 338, 573. doi: 10.1016/j.jmb.2004.02.055
106. [106]
  (106) Sadqi, M.; de Alba, E.; Perez-Jimenez, R.; Sanchez-Ruiz, J.M.; Muñoz, V. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 4127.doi: 10.1073/pnas.0812108106
107. [107]
  (107) Watters, A. L.; Deka, P.; Corrent, C.; Callender, D.; Varani, G.;Sosnick, T.; Baker, D. Cell 2007, 128, 613. doi: 10.1016/j.cell.2006.12.042
108. [108]
  (108) Zhang, Z.; Chan, H. S. Biophys. J. 2009, 96, L25.
109. [109]
  (109) Zhang, Z.; Chan, H. S. Proc. Natl. Acad. Sci. U. S. A. 2010,107, 2920. doi: 10.1073/pnas.0911844107
110. [110]
  (110) Qi, Y.; Huang, Y.; Liang, H.; Liu, Z.; Lai, L. Biophys. J. 2010,98, 321. doi: 10.1016/j.bpj.2009.10.018
1. [1]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
2. [2]
  Jinfu Ma , Hui Lu , Jiandong Wu , Zhongli Zou . Teaching Design of Electrochemical Principles Course Based on “Cognitive Laws”: Kinetics of Electron Transfer Steps. University Chemistry, 2024, 39(3): 174-177. doi: 10.3866/PKU.DXHX202309052
3. [3]
  Shule Liu . Application of SPC/E Water Model in Molecular Dynamics Teaching Experiments. University Chemistry, 2024, 39(4): 338-342. doi: 10.3866/PKU.DXHX202310029
4. [4]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
5. [5]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044
6. [6]
  Xuzhen Wang , Xinkui Wang , Dongxu Tian , Wei Liu . Enhancing the Comprehensive Quality and Innovation Abilities of Graduate Students through a “Student-Centered, Dual Integration and Dual Drive” Teaching Model: A Case Study in the Course of Chemical Reaction Kinetics. University Chemistry, 2024, 39(6): 160-165. doi: 10.3866/PKU.DXHX202401074
7. [7]
  Dexin Tan , Limin Liang , Baoyi Lv , Huiwen Guan , Haicheng Chen , Yanli Wang . Exploring Reverse Teaching Practices in Physical Chemistry Experiment Courses: A Case Study on Chemical Reaction Kinetics. University Chemistry, 2024, 39(11): 79-86. doi: 10.12461/PKU.DXHX202403048
8. [8]
  Yiying Yang , Dongju Zhang . Elucidating the Concepts of Thermodynamic Control and Kinetic Control in Chemical Reactions through Theoretical Chemistry Calculations: A Computational Chemistry Experiment on the Diels-Alder Reaction. University Chemistry, 2024, 39(3): 327-335. doi: 10.3866/PKU.DXHX202309074
9. [9]
  Yue Wu , Jun Li , Bo Zhang , Yan Yang , Haibo Li , Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028
10. [10]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
11. [11]
  You Wu , Chang Cheng , Kezhen Qi , Bei Cheng , Jianjun Zhang , Jiaguo Yu , Liuyang Zhang . ZnO/D-A共轭聚合物S型异质结高效光催化产H₂O₂及其电荷转移动力学研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2406027-. doi: 10.3866/PKU.WHXB202406027
12. [12]
  Yan Li , Xinze Wang , Xue Yao , Shouyun Yu . Kinetic Resolution Enabled by Photoexcited Chiral Copper Complex-Mediated Alkene E→Z Isomerization: A Comprehensive Chemistry Experiment for Undergraduate Students. University Chemistry, 2024, 39(5): 1-10. doi: 10.3866/PKU.DXHX202309053
13. [13]
  Wanqun Hu , Pingping Zhu , Yuan Zheng , Wanqun Zhang , Wei Shao , Hong Wu , Qiang Zhou , Kaiping Yang , Xiang Sheng . Design and Practice of Ideological and Political Case Study in Instrumental Analysis Experiment Course: the Extraction and Structural Identification of Artemisinin. University Chemistry, 2024, 39(2): 203-207. doi: 10.3866/PKU.DXHX202310062
14. [14]
  Xuan Zhou , Yi Fan , Zhuoqi Jiang , Zhipeng Li , Guowen Yuan , Laiying Zhang , Xu Hou . Liquid Gating Mechanism and Basic Properties Characterization: a New Experimental Design for Interface and Surface Properties in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 113-120. doi: 10.12461/PKU.DXHX202407111
15. [15]
  Li Jiang , Changzheng Chen , Yang Su , Hao Song , Yanmao Dong , Yan Yuan , Li Li . Electrochemical Synthesis of Polyaniline and Its Anticorrosive Application: Improvement and Innovative Design of the “Chemical Synthesis of Polyaniline” Experiment. University Chemistry, 2024, 39(3): 336-344. doi: 10.3866/PKU.DXHX202309002
16. [16]
  Qi Wang , Yicong Gao , Feng Lu , Quli Fan . Preparation and Performance Characterization of the Second Near-Infrared Phototheranostic Probe: A New Design and Teaching Practice of Polymer Chemistry Comprehensive Experiment. University Chemistry, 2024, 39(11): 342-349. doi: 10.12461/PKU.DXHX202404141
17. [17]
  Yan Liu , Yuexiang Zhu , Luhua Lai . Introduction to Blended and Small-Class Teaching in Structural Chemistry: Exploring the Structure and Properties of Crystals. University Chemistry, 2024, 39(3): 1-4. doi: 10.3866/PKU.DXHX202306084
18. [18]
  Zitong Chen , Zipei Su , Jiangfeng Qian . Aromatic Alkali Metal Reagents: Structures, Properties and Applications. University Chemistry, 2024, 39(8): 149-162. doi: 10.3866/PKU.DXHX202311054
19. [19]
  Ruming Yuan , Pingping Wu , Laiying Zhang , Xiaoming Xu , Gang Fu . Patriotic Devotion, Upholding Integrity and Innovation, Wholeheartedly Nurturing the New: The Ideological and Political Design of the Experiment on Determining the Thermodynamic Functions of Chemical Reactions by Electromotive Force Method. University Chemistry, 2024, 39(4): 125-132. doi: 10.3866/PKU.DXHX202311057
20. [20]
  Kun WANG , Wenrui LIU , Peng JIANG , Yuhang SONG , Lihua CHEN , Zhao DENG . Hierarchical hollow structured BiOBr-Pt catalysts for photocatalytic CO₂ reduction. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1270-1278. doi: 10.11862/CJIC.20240037

Get Citation

PDF

XML

Metrics

PDF Downloads(659)
Abstract views(1978)
HTML views(2)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142