Citation: FU Xiao-Yi, SHAO Guang-Sheng, HAN Rong-Cheng, MA Yan, XUE Fu-Min, YANG Fan, FU Li-Min, ZHANG Jian-Ping, WANG Yuan. Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging[J]. Acta Physico-Chimica Sinica, ;2012, 28(10): 2480-2486. doi: 10.3866/PKU.WHXB201208161 shu

Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

1.
1 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
2 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China;
3 Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China

Received Date: 20 June 2012
Available Online: 16 August 2012
Fund Project: 国家自然科学基金(21073002, 21133001, 21227803, 51121091) (21073002, 21133001, 21227803, 51121091)国家重点基础研究专项经费(2011CB808702)资助 (2011CB808702)

Luminescent nanospheres (EuPHS, d_av=45 nm) containing 40% (w) of Eu(tta)₃dpbt (tta= thenoyltrifluoroacetonato;dpbt=2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine) were prepared by encapsulating Eu(tta)₃dpbt in a hybrid matrix formed in situ from poly(styrene-co-methyl methacrylate), octyltrimethoxysilane, and poly(octylsiloxane). The EuPHS are promising luminescent markers for bioanalysis because of their od dispersibility in aqueous solutions, high photostability, low cytotoxicity, and bright Eu³⁺ luminescence under excitation at long wavelengths. EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu³⁺ luminescence properties, with a visible-light excitation peak at 415 nm and an excitation window extending up to 475 nm. The quantum yield for Eu³⁺ luminescence was 0.31 (λ_ex=415 nm, T=23 ℃), and the two-photon excitation (TPE) action cross section was 5.0×10⁵ GM (1 GM=10^-50 cm⁴·s·photo^-1×particle^-1) at 830 nm. Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.
- Luminescence,
- Europium complex,
- Two-photon excitation,
- Bio-nanoprobe,
- Cell Imaging
1. [1]
  
  (1) Helmchen, F.; Denk,W. Nat. Methods 2005, 2, 932. doi: 10.1038/nmeth818
2. [2]
  (2) Denk,W.; Strickler, J. H.;Webb,W.W. Science 1990, 248, 73.doi: 10.1126/science.2321027
3. [3]
  (3) Eliseeva, S. V.; Bunzli, J. C. G. Chem. Soc. Rev. 2010, 39, 189.doi: 10.1039/b905604c
4. [4]
  (4) Ma, Y.;Wang, Y. Coord. Chem. Rev. 2010, 254, 972. doi: 10.1016/j.ccr.2010.02.013
5. [5]
  (5) Andraud, C.; Maury, O. Eur. J. Inorg. Chem. 2009, 4357.
6. [6]
  (6) Fu, L. M.;Wen, X. F.; Ai, X. C.; Sun, Y.;Wu, Y. S.; Zhang, J. P.;Wang, Y. Angew. Chem. Int. Edit. 2005, 44, 747. doi: 10.1002/(ISSN)1521-3773
7. [7]
  (7) Piszczek, G.; Maliwal, B. P.; Gryczynski, I.; Dattelbaum, J.;Lakowicz, J. R. J. Fluoresc. 2001, 11, 101. doi: 10.1023/A:1016673300913
8. [8]
  (8) Picot, A.; D'Aleo, A.; Baldeck, P. L.; Grichine, A.; Duperray, A.;Andraud, C.; Maury, O. J. Am. Chem. Soc. 2008, 130, 1532. doi: 10.1021/ja076837c
9. [9]
  (9) Eliseeva, S. V.; Aubock, G.; van Mourik, F.; Cannizzo, A.;Song, B.; Deiters, E.; Chauvin, A. S.; Chergui, M.; Bunzli, J. C.G. J. Phys. Chem. B 2010, 114, 2932. doi: 10.1021/jp9090206
10. [10]
  (10) Shi, M.; Ding, C. R.; Dong, J.W.;Wang, H. Z.; Tian, Y. P.; Hu,Z. J. Phys. Chem. Chem. Phys. 2009, 11, 5119.
11. [11]
  (11) Lakowicz, J. R.; Piszczek, G.; Maliwal, B. P.; Gryczynski, I.ChemPhysChem 2001, 2, 247. doi: 10.1002/(ISSN)1439-7641
12. [12]
  (12) Werts, M. H. V.; Nerambourg, N.; Pelegry, D.; Le Grand, Y.;Blanchard-Desce, M. Photochem. Photobiol. Sci. 2005, 4, 531.doi: 10.1039/b504495b
13. [13]
  (13) Picot, A.; Malvolti, F.; Le Guennic, B.; Baldeck, P. L.;Williams,J. A. G.; Andraud, C.; Maury, O. Inorg. Chem. 2007, 46, 2659.doi: 10.1021/ic062181x
14. [14]
  (14) D'Aleo, A.; Picot, A.; Baldeck, P. L.; Andraud, C.; Maury, O.Inorg. Chem. 2008, 47, 10269. doi: 10.1021/ic8012975
15. [15]
  (15) D'Aleo, A.; Allali, M.; Picot, A.; Baldeck, P. L.; Toupet, L.;Andraud, C.; Maury, O. C. R. Chimie 2010, 13, 681. doi: 10.1016/j.crci.2010.01.008
16. [16]
  (16) Xue, F. M.; Ma, Y.; Fu, L. M.; Hao, R.; Shao, G. S.; Tang, M.X.; Zhang, J. P.;Wang, Y. Phys. Chem. Chem. Phys. 2010, 12,3195.
17. [17]
  (17) Palsson, L. O.; Pal, R.; Murray, B. S.; Parker, D.; Beeby, A.Dalton Trans. 2007, 5726.
18. [18]
  (18) Law, G. L.;Wong, K. L.; Man, C.W. Y.; Tsao, S.W.;Wong,W.T. J. Biophotonics 2009, 2, 718. doi: 10.1002/jbio.v2:12
19. [19]
  (19) Wu, J.; Ye, Z. Q.;Wang, G. L.; Jin, D. Y.; Yuan, J. L.; Guan, Y.F.; Piper, J. J. Mater. Chem. 2009, 19, 1258. doi: 10.1039/b815999h
20. [20]
  (20) Shao, G. S.; Han, R. C.; Ma, Y.; Tang, M. X.; Xue, F. M.; Sha,Y. L.;Wang, Y. Chem. Eur. J. 2010, 16, 8647. doi: 10.1002/chem.201001367
21. [21]
  (21) Yang, C.; Fu, L. M.;Wang, Y.; Zhang, J. P.;Wong,W. T.; Ai, X.C.; Qiao, Y. F.; Zou, B. S.; Gui, L. L. Angew. Chem. Int. Edit.2004, 43, 5010. doi: 10.1002/(ISSN)1521-3773
22. [22]
  (22) Wen, X. F.; Li, M. Y.;Wang, Y.; Zhang, J. P.; Fu, L. M.; Hao, R.;Ma, Y.; Ai, X. C. Langmuir 2008, 24, 6932. doi: 10.1021/la800903s
23. [23]
  (23) Shao, G. S.; Xue, F. M.; Han, R. C.; Tang, M. X.;Wang, Y. ActaPhys. -Chim. Sin. 2010, 26, 2031. [邵光胜, 薛富民, 韩荣成,汤敏贤, 王远. 物理化学学报, 2010, 26, 2031.] doi: 10.3866/PKU.WHXB20100715
24. [24]
  (24) Hao, R.; Li, M.;Wang, Y.; Zhang, J.; Ma, Y.; Fu, L.;Wen, X.;Wu, Y.; Ai, X.; Zhang, S.;Wei, Y. Adv. Funct. Mater. 2007, 17,3663. doi: 10.1002/(ISSN)1616-3028
25. [25]
  (25) Arriagada, F. J.; Osseo-Asare, K. J. Colloid Interface Sci. 1999,211, 210. doi: 10.1006/jcis.1998.5985
26. [26]
  (26) Stöber,W.; Fink, A.; Bohn, E. J. Colloid Interface Sci. 1968, 26,62. doi: 10.1016/0021-9797(68)90272-5
27. [27]
  (27) Wang, Y.; Fu, X. Y.; Shao, G. S. Photoluminescent nanoparticle,preparation, and application thereof. CN Patent,200910203407.3, 2009-05-05. [王远, 符小艺, 邵光胜. 荧光纳米粒子及其制备方法和应用: 中国, CN200910203407.3[P]2009-05-05.]
28. [28]
  (28) Peng, H. S.; Stich, M. I. J.; Yu, J. B.; Sun, L. N.; Fischer, L. H.;Wolfbeis, O. S. Adv. Mater. 2010, 22, 716. doi: 10.1002/adma.v22:6
29. [29]
  (29) Demas, J. N.; Crosby, G. A. J. Phys. Chem. 1971, 75, 991. doi: 10.1021/j100678a001
30. [30]
  (30) Larson, D. R.; Zipfel,W. R.;Williams, R. M.; Clark, S.W.;Bruchez, M. P.;Wise, F.W.;Webb,W.W. Science 2003, 300,1434. doi: 10.1126/science.1083780
31. [31]
  (31) Chan,W. C.W.; Nie, S. M. Science 1998, 281, 2016. doi: 10.1126/science.281.5385.2016
32. [32]
  (32) Richard, J. P.; Melikov, K.; Brooks, H.; Prevot, P.; Lebleu, B.;Chernomordik, L. V. J. Biol. Chem. 2005, 280, 15300. doi: 10.1074/jbc.M401604200
33. [33]
  (33) Kam, N.W. S.; Liu, Z. A.; Dai, H. J. Angew. Chem. Int. Edit.2006, 45, 577. doi: 10.1002/(ISSN)1521-3773
34. [34]
  (34) Zhang, B. L.; Li, Y. Q.; Fang, C. Y.; Chang, C. C.; Chen, C. S.;Chen, Y. Y.; Chang, H. C. Small 2009, 5, 2716. doi: 10.1002/smll.v5:23
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Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

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Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging