Citation: FU Xiao-Yi, SHAO Guang-Sheng, HAN Rong-Cheng, MA Yan, XUE Fu-Min, YANG Fan, FU Li-Min, ZHANG Jian-Ping, WANG Yuan. Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging[J]. Acta Physico-Chimica Sinica, ;2012, 28(10): 2480-2486. doi: 10.3866/PKU.WHXB201208161 shu

Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

1.
1 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
2 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China;
3 Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China

Received Date: 20 June 2012
Available Online: 16 August 2012
Fund Project: 国家自然科学基金(21073002, 21133001, 21227803, 51121091) (21073002, 21133001, 21227803, 51121091)国家重点基础研究专项经费(2011CB808702)资助 (2011CB808702)

Luminescent nanospheres (EuPHS, d_av=45 nm) containing 40% (w) of Eu(tta)₃dpbt (tta= thenoyltrifluoroacetonato;dpbt=2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine) were prepared by encapsulating Eu(tta)₃dpbt in a hybrid matrix formed in situ from poly(styrene-co-methyl methacrylate), octyltrimethoxysilane, and poly(octylsiloxane). The EuPHS are promising luminescent markers for bioanalysis because of their od dispersibility in aqueous solutions, high photostability, low cytotoxicity, and bright Eu³⁺ luminescence under excitation at long wavelengths. EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu³⁺ luminescence properties, with a visible-light excitation peak at 415 nm and an excitation window extending up to 475 nm. The quantum yield for Eu³⁺ luminescence was 0.31 (λ_ex=415 nm, T=23 ℃), and the two-photon excitation (TPE) action cross section was 5.0×10⁵ GM (1 GM=10^-50 cm⁴·s·photo^-1×particle^-1) at 830 nm. Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.
- Luminescence,
- Europium complex,
- Two-photon excitation,
- Bio-nanoprobe,
- Cell Imaging
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Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

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Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging