Citation: HE Chuan-Xin, YUAN An-Peng, ZHANG Qian-Lin, REN Xiang-Zhong, LI Cui-Hua, LIU Jian-Hong. Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on ld Surface and Its Sensor Application[J]. Acta Physico-Chimica Sinica doi: 10.3866/PKU.WHXB201207191 shu

Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on ld Surface and Its Sensor Application

1.
Shenzhen Key Laboratory of Functional Polymer, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen518060, Guangdong Province, P. R. China

Received Date: 14 May 2012
Available Online: 19 July 2012
Fund Project: 国家自然科学基金青年基金(21004040) (21004040)深圳大学校基金(201102)资助项目 (201102)

Core-shell poly(methyl methacrylate)-bovine serum albumin (PMMA-BSA) nanoparticles with PMMA cores and BSA shells were prepared via a copper ion-mediated initiation system. The core-shell structure of the nanoparticles was characterized by transmission electron microscopy (TEM) and the surface compositions of the nanoparticles were tested by X-ray photoelectron spectroscopy (XPS), which further demonstrated that the particles have a BSA protein shell. The adsorption of these PMMA-BSA particles onto ld surfaces was studied by quartz crystal microbalance with dissipation (QCM-D). The significantly change of frequency shift and dissipation factor indicated that PMMA-BSA particles are adsorbed on the ld surface. The repeated buffer washing had nearly no effect on either frequency shift or dissipation factor, revealing that the adsorption is fairly strong. An amperometric glucose biosensor was constructed by immobilizing glucose oxidase on PMMA-BSA particles modified with glutaraldehyde, using a ld electrode as a substrate on which to adsorb the PMMA-BSA particles. Electrochemical measurements show this biosensor exhibited a od current response to glucose. Working at 0.3 V, the biosensor had a short response time of 11 s, a sensitivity of 28.6 μA·L^-1·mmol^-1·cm^-2 and a linear range from 0.2 to 5.85 mmol·L^-1 with a correlation coefficient of 0.989. After storage at 25℃ for one month, the sensor response current decreased by only 16%, thus showing od thermal stability.
- Glucose oxidase,
- Biosensor,
- Quartz crystal microbalance,
- Frequency,
- Dissipation,
- Adsorption
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