Citation: WU Rui-Guang, CHEN Lin, YU Zhi-Wu. Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study[J]. Acta Physico-Chimica Sinica, ;2012, 28(08): 2008-2014. doi: 10.3866/PKU.WHXB201205171 shu

Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study

WU Rui-Guang ^1,2 ,
CHEN Lin ¹ ,
YU Zhi-Wu ^1,

1.
1. Key Laboratory of Bioorganic Phosphorous Chemistry & Chemical Biology of the Ministry of Education, Department of Chemistry, Beijing 100084, P. R. China;
2. School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, P. R. China

Received Date: 9 April 2012
Available Online: 17 May 2012
Fund Project: 国家自然科学基金(20633080, 21133009)资助项目 (20633080, 21133009)

The properties of the liquid ordered (L_o) phase of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/stigmasterol liposomes were investigated using synchrotron small-angle and wide-angle X-ray diffraction techniques. The lamellar repeat distance of the L_o phase, derived from small-angle X-ray scattering experiments, changes slightly with sterol concentration and temperature. While the d-spacings of the acyl chains, evaluated from wide-angle X-ray scattering data, show a much broader range than those of the gel and liquid-crystal phases, varying from 0.422 to 0.460 nm when the temperature increases from 30 to 52 °C. The electron density profiles show that the bilayer thickness and the water-layer thickness of the liquid-ordered phase are larger than that of the liquid-crystal phase, and the bilayer thickness of the liquid-ordered phase decreases with increasing temperature. The results of this study are of help in understanding the phase state and the ordered structure of biomembranes.
- Liquid ordered phase,
- Lipid raft,
- Stigmasterol,
- 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine,
- X-ray diffraction,
- Small-angle scattering,
- Electron density
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