Citation: LI Jin-Hua, LI JING, FANG Xuan, WANG Xiao-Hua, WEI Zhi-Peng. Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate[J]. Acta Physico-Chimica Sinica, ;2012, 28(06): 1393-1397. doi: 10.3866/PKU.WHXB201204062 shu

Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

LI Jin-Hua ^1,2, ,
LI JING ¹ ,
FANG Xuan ^1,2 ,
WANG Xiao-Hua ¹ ,
WEI Zhi-Peng ¹

1.
1. School of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China;
2. International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology, Changchun 130022, P. R. China

Received Date: 9 March 2012
Available Online: 6 April 2012
Fund Project: 国家自然科学基金(61006065, 61076039, 10804071) (61006065, 61076039, 10804071) 高等学校博士学科点专项科研基金(20102216110001, 20112216120005) (20102216110001, 20112216120005) 吉林省科技发展计划(20091039, 20090555, 20101546, 20121816, 201201116) (20091039, 20090555, 20101546, 20121816, 201201116)吉林省教育厅项目(JYT2011-05, JYT2011-10, JYT2011-11)资助 (JYT2011-05, JYT2011-10, JYT2011-11)

Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase ( x). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). x was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the x and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and x can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry.
- Glucose oxidase,
- Flexible substrate,
- Biosensor,
- ZnO
1. [1]
  (1) Yu, J.; Ju, H. Anal. Chem. 2002, 74, 3579. doi: 10.1021/ac011290k
2. [2]
  (2) Lee, H.; Yoon, S. W.; Kim, E. J.; Park, J. Nano Lett. 2007, 7, 778. doi: 10.1021/nl0630539
3. [3]
  (3) Vidur, K. L. Melliand-China 2005, 2,75
4. [4]
  (4) Rajesh, C.; Kaneto, K. Curr. Appl. Phys. 2005, 2, 178
5. [5]
  (5) Wang, J.; Fang, L.; Lopez, D. Analyst 1994, 119, 455
6. [6]
  (6) Consnier, S.; Innocent, C. Bioelectrochem. Bioenergy 1993, 3, 147
7. [7]
  (7) Kondo, T.; Horiuchi, S.; Yagi, I.; Ye, S.; Uosaki, K. J. Am. Chem. Soc 121 1999, 121, 391
8. [8]
  (8) Liu, Z. J.; Liu, B. H.; Kong, J. L.; Deng, J. Q. Anal. Chem. 2000, 72, 707
9. [9]
  (9) Wang, B. Q.; Li, B.; Wang, Z. X.; Xu, G. B.; Wang, Q.; Dong, S. J. Anal. Chem 1999, 71,1935
10. [10]
  (10) Muguruma, H.; Hiratsuka, A.; Karube, I. Anal. Chem 2000, 72, 2671
11. [11]
  (11) Yang, Q.; Atanasov, P.; Wilkins, E. Sens. Actuators B: Chem 1998, 46, 249
12. [12]
  (12) Jua, H. Q.; Liua, S. Q.; Geb, B. X.; Lisdatb, F.; Schellerb, F. W. Electroanalysis 2002, 14,141
13. [13]
  (13) Huang, M. H.; Mao, S.; Feick, H.; Yan, H.; Wu, Y.; Kind, H.; Weber, E.; Russo, R.; Yang, P. Science 2001, 292,1897. doi: 10.1126/science.1060367
14. [14]
  (14) Xu, C. X.; Sun, X. W. Appl. Phys. Lett. 2003, 83, 3806
15. [15]
  (15) Gao, P. X.; Ding, Y.; Mai, W.; Hughes, W. L.; Lao, C. S.; Wang, Z. L. Science 2005, 309, 1700
16. [16]
  (16) Sun, X. W.; Kwok, H. S. J. Appl. Phys 1999, 86, 408
17. [17]
  (17) Zhang, F.; Wang, X.; Ai, S.; Sun, Z.; Wan, Q.; Zhu, Z.; Xian, Y.; Jin, L.; Yamamoto, K. Anal. Chim. Acta 2004, 519, 155
18. [18]
  (18) Umar, A.; Rahman, M. M.; Kim, S. H.; Hahn, Y. B. J. Nanosci. Nanotechnol 2008, 8, 3216
19. [19]
  (19) Wei, A. Suna, X. W.; Wang, J. X.; Lei, Y.; Cai, X. P.; Li, C. M.; Dong, Z. L., Huang, W. Appl. Phys. Lett. 2006, 89, 123902
20. [20]
  (20) Liu, Z.; Liu, Y.; Yang, H.; Yang, Y.; Shen, G.; Yu, R. Electroanalysis 2005, 17, 1065
21. [21]
  (21) Cullity, B. D.; Stock, S. R. Elements of X-Ray Diffraction; Prentice Hall: New Jersey, 2001; p 619.
22. [22]
  (22) Topoglidis, E.; Cass, A. E. G.; O'Regan, B.; Durrant, J. R. J. Electroanal. Chem. 2001, 517, 20
23. [23]
  (23) Schvartzman, M.; Sidorov, V.; Ritter, D.; Paz, Y. J. Vac. Sci. Technol. 2001, 169, 68
24. [24]
  (24) Liu, T.; Liao, H.; Lin, C.; Hu, S.; Chen, S. Langmuir 2006, 22, 5804
25. [25]
  (25) Cao, X.; Wang, N.; Li, L.; Guo, L. Sensors and Actuators B 2008, 129, 268
26. [26]
  (26) Chi, Q.; Zhang, J.; Dong, S.; Wang, E. J. Chem. Soc. Faraday. Trans 1994, 90, 2057
27. [27]
  (27) Weber, J.; Jeedigunta, S.; Kumar, A. Journal of Nanomaterials 2008, 1
28. [28]
  (28) Dai, Z.; Liu, K.; Tang, Y.; Yang, X.; Bao, J.; Shen, J. J. Mater. Chem. 2008, 18, 1919
29. [29]
  (29) Kong, T.; Chen, Y.; Ye, Y.; Zhang, K.; Wang, H. Z.; Wang, X. Sens. Actuators B 2009, 38, 344
1. [1]
  Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101
2. [2]
  Yu Wang , Shoulei Zhang , Tianming Lv , Yan Su , Xianyu Liu , Fuping Tian , Changgong Meng . Introduce a Comprehensive Inorganic Synthesis Experiment: Synthesis of Nano Zinc Oxide via Microemulsion Using Waste Soybean Oil. University Chemistry, 2024, 39(7): 316-321. doi: 10.3866/PKU.DXHX202311035
3. [3]
  Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
4. [4]
  Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
5. [5]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
6. [6]
  Xiaoning TANG , Shu XIA , Jie LEI , Xingfu YANG , Qiuyang LUO , Junnan LIU , An XUE . Fluorine-doped MnO₂ with oxygen vacancy for stabilizing Zn-ion batteries. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1671-1678. doi: 10.11862/CJIC.20240149
7. [7]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
8. [8]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
9. [9]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
10. [10]
  Minna Ma , Yujin Ouyang , Yuan Wu , Mingwei Yuan , Lijuan Yang . Green Synthesis of Medical Chemiluminescence Reagents by Photocatalytic Oxidation. University Chemistry, 2024, 39(5): 134-143. doi: 10.3866/PKU.DXHX202310093
11. [11]
  Yunting Shang , Yue Dai , Jianxin Zhang , Nan Zhu , Yan Su . Something about RGO (Reduced Graphene Oxide). University Chemistry, 2024, 39(9): 273-278. doi: 10.3866/PKU.DXHX202306050
12. [12]
  Linbao Zhang , Weisi Guo , Shuwen Wang , Ran Song , Ming Li . Electrochemical Oxidation of Sulfides to Sulfoxides. University Chemistry, 2024, 39(11): 204-209. doi: 10.3866/PKU.DXHX202401009
13. [13]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
14. [14]
  Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
15. [15]
  Lin Song , Dourong Wang , Biao Zhang . Innovative Experimental Design and Research on Preparing Flexible Perovskite Fluorescent Gels Using 3D Printing. University Chemistry, 2024, 39(7): 337-344. doi: 10.3866/PKU.DXHX202310107
16. [16]
  Xiaofeng Zhu , Bingbing Xiao , Jiaxin Su , Shuai Wang , Qingran Zhang , Jun Wang . Transition Metal Oxides/Chalcogenides for Electrochemical Oxygen Reduction into Hydrogen Peroxides. Acta Physico-Chimica Sinica, 2024, 40(12): 2407005-. doi: 10.3866/PKU.WHXB202407005
17. [17]
  Kai CHEN , Fengshun WU , Shun XIAO , Jinbao ZHANG , Lihua ZHU . PtRu/nitrogen-doped carbon for electrocatalytic methanol oxidation and hydrogen evolution by water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1357-1367. doi: 10.11862/CJIC.20230350
18. [18]
  Zhuo WANG , Junshan ZHANG , Shaoyan YANG , Lingyan ZHOU , Yedi LI , Yuanpei LAN . Preparation and photocatalytic performance of CeO₂-reduced graphene oxide by thermal decomposition. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1708-1718. doi: 10.11862/CJIC.20240067
19. [19]
  Yongmei Liu , Lisen Sun , Zhen Huang , Tao Tu . Curriculum-Based Ideological and Political Design for the Experiment of Methanol Oxidation to Formaldehyde Catalyzed by Electrolytic Silver. University Chemistry, 2024, 39(2): 67-71. doi: 10.3866/PKU.DXHX202308020
20. [20]
  Hui Shi , Shuangyan Huan , Yuzhi Wang . Ideological and Political Design of Potassium Permanganate Oxidation-Reduction Titration Experiment. University Chemistry, 2024, 39(2): 175-180. doi: 10.3866/PKU.DXHX202308042

Get Citation

PDF

XML

Metrics

PDF Downloads(816)
Abstract views(2319)
HTML views(40)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142