Citation: LI Mi, LIU Lian-Qing, XI Ning, WANG Yue-Chao, DONG Zai-Li, XIAO Xiu-Bin, ZHANG Wei-Jing. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy[J]. Acta Physico-Chimica Sinica, ;2012, 28(06): 1502-1508. doi: 10.3866/PKU.WHXB201203201 shu

Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy

LI Mi ^1,2 ,
LIU Lian-Qing ^1, ,
XI Ning ^1,3, ,
WANG Yue-Chao ¹ ,
DONG Zai-Li ¹ ,
XIAO Xiu-Bin ⁴ ,
ZHANG Wei-Jing ^4,

1.
1. State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, P. R. China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
3. Department of Electrical and Computer Engineering, Michigan State University, East Lansing 48824, USA;
4. Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, P. R. China

Received Date: 9 March 2012
Available Online: 20 March 2012
Fund Project: 国家自然科学基金(60904095, 61175103) (60904095, 61175103) 国家高技术研究发展计划项目(863) (2009AA03Z316) (863) (2009AA03Z316)

Atomic force microscopy (AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions. However, owing to the lack of adequate cellular immobilization methods, AFM imaging of living, suspended mammalian cells is still a big challenge. In this paper, a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine. In this way, the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically. When the cell is stimulated by 0.2 mg·mL^-1 Rituximab for 2 h, the cell topography becomes more corrugated and Young's modulus decreases from 196 to 183 kPa. When the cell is stimulated by 0.5 mg·mL^-1 Rituximab for 2 h, the cell topography changes more significantly and some tubercles appear, and Young's modulus decreases from 234 to 175 kPa. These results thus provide a unique insight into the effects of Rituximab on individual cells.
- Atomic force microscopy,
- Lymphoma,
- Elasticity,
- Force curve,
- Young's modulus
References
1. [1]
  (1) Binnig, G.; Quate, C. F.; Gerber, C. Phys. Rev. Lett. 1986, 56, 930. doi: 10.1103/PhysRevLett.56.930
2. [2]
  (2) Dufrene, Y. F. Nat. Rev. Microbiol. 2004, 2, 451. doi: 10.1038/nrmicro905
3. [3]
  (3) Muller, D. J.; Dufrene, Y. F. Nat. Nanotechnol. 2008, 3, 261. doi: 10.1038/nnano.2008.100
4. [4]
  (4) Lee, S.; Mandic, J.; Vliet, K. J. V. Proc. Natl. Acad. Sci. U. S. A. 2007, 104, 9609. doi: 10.1073/pnas.0702668104
5. [5]
  (5) Cross, S. E.; Jin, Y. S.; Rao, J.; Gimzewski, J. K. Nat. Nanotechnol. 2007, 2, 780. doi: 10.1038/nnano.2007.388
6. [6]
  (6) Neuman, K. C.; Nagy, A. Nature Methods 2008, 5, 491. doi: 10.1038/nmeth.1218
7. [7]
  (7) Stewart, M. P.; Helenius, J.; Toyoda, Y.; Ramanathan, S. P.; Muller, D. J.; Hyman, A. A. Nature 2011, 469, 226. doi: 10.1038/nature09642
8. [8]
  (8) Uchihashi, T.; Iino, R.; Ando, T.; Noji, H. Science 2011, 333, 755. doi: 10.1126/science.1205510
9. [9]
  (9) Butt, H. J.; Wolff, E. K.; uld, S. A. C.; Northern, B. D.; Peterson, C. M.; Hansma, P. K. J. Struct. Biol. 1990, 105, 54. doi: 10.1016/1047-8477(90)90098-W
10. [10]
  (10) Chen, P. P.; Dong, H. T.; Chen, L.; Sun, Q. M.; Han, D. Chin. Sci. Bull. 2009, 54, 2410. doi: 10.1007/s11434-009-0374-1
11. [11]
  (11) Wu, Y.; Lu, H.; Cai, J.; He, X.; Hu, Y.; Zhao, H.; Wang, X. Nanoscale Res. Lett. 2009, 4, 942. doi: 10.1007/s11671-009-9340-8
12. [12]
  (12) Puntheeranurak, T.; Wildling, L.; Gruber, H. J.; Kinne, R. K. H.; Hinterdorfer, P. J. Cell Sci. 2006, 119, 2960. doi: 10.1242/jcs.03035
13. [13]
  (13) Kirat, K. E.; Burton, I.; Dupres, V.; Dufrene, Y. F. J. Microsc. 2005, 218, 199. doi: 10.1111/j.1365-2818.2005.01480.x
14. [14]
  (14) Lam, W. A.; Rosenbluth, M. J.; Fletcher, D. A. Blood 2007, 109, 3505. doi: 10.1182/blood-2006-08-043570
15. [15]
  (15) Ng, L.; Hung, H. H.; Sprunt, A.; Chubinskaya, S.; Ortiz, C.; Grodzinsky, A. J. Biomech. 2007, 40, 1011. doi: 10.1016/j.jbiomech.2006.04.004
16. [16]
  (16) Park, S.; Koch, D.; Cardenas, R.; Kas, J.; Shih, C. K. Biophys. J. 2005, 89, 4330. doi: 10.1529/biophysj.104.053462
17. [17]
  (17) Martens, J. C.; Radmacher, M. Pflugers Archiv-European Journal of Physiology 2008, 456, 95. doi: 10.1007/s00424-007-0419-8
18. [18]
  (18) Lulevich, V.; Yang, H.; Isseroff, R. R.; Liu, G. Ultramicroscopy 2010, 110, 1435. doi: 10.1016/j.ultramic.2010.07.009
19. [19]
  (19) Cai, X.; Yang, X.; Cai, J.; Wu, S.; Chen, Q. J. Phys. Chem. B 2010, 114, 3833. doi: 10.1021/jp910682q
20. [20]
  (20) Kirmizis, D.; Lo thetidis, S. Int. J. Nanomed. 2010, 5, 137.
21. [21]
  (21) Leporatti, S.; Gerth, A.; Kohler, G.; Kohlstrunk, B.; Hauschildt, S.; Donath, E. FEBS Lett. 2006, 580, 450. doi: 10.1016/j.febslet.2005.12.037
22. [22]
  (22) Deng, Z.; Lulevich, V.; Liu, F.; Liu, G. J. Phys. Chem. B 2010, 114, 5971. doi: 10.1021/jp9114546
23. [23]
  (23) Muller, D. J.; Dufrene, Y. F. Trends Cell Biol. 2011, 21, 461. doi: 10.1016/j.tcb.2011.04.008
24. [24]
  (24) Muller, D. J.; Dufrene, Y. F. Curr. Biol. 2011, 21, R212.
25. [25]
  (25) Li, M.; Liu, L. Q.; Xi, N.; Wang, Y. C.; Dong, Z. L.; Xiao, X. B.; Zhang, W. J. Chin. Sci. Bull. 2010, 55, 2188. [李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 科学通报, 2010, 55, 2188.] doi: 10.1360/972010-218
26. [26]
  (26) Touhami, A.; Nysten, B.; Dufrene, Y. F. Langmuir 2003, 19, 4539. doi: 10.1021/la034136x
27. [27]
  (27) Cartron, G.; Watier, H.; lay, J.; Solal-Celigny, P. Blood 2004, 104, 2635. doi: 10.1182/blood-2004-03-1110
28. [28]
  (28) Bonavida, B. Oncogene 2007, 26, 3629. doi: 10.1038/sj.onc.1210365
29. [29]
  (29) Smith, M. R. Oncogene 2003, 22, 7359. doi: 10.1038/sj.onc.1206939
30. [30]
  (30) Hu, M.; Wang, J.; Zhao, H.; Dong, S.; Cai, J. J. Biomech. 2009, 42, 1513. doi: 10.1016/j.jbiomech.2009.03.051
31. [31]
  (31) Li, M.; Liu, L.; Xi, N.; Wang, Y.; Dong, Z.; Tabata, O.; Xiao, X.; Zhang, W. Biochem. Biophys. Res. Commu. 2011, 404, 689. doi: 10.1016/j.bbrc.2010.12.043
1. [1]
  (1) Binnig, G.; Quate, C. F.; Gerber, C. Phys. Rev. Lett. 1986, 56, 930. doi: 10.1103/PhysRevLett.56.930
2. [2]
  (2) Dufrene, Y. F. Nat. Rev. Microbiol. 2004, 2, 451. doi: 10.1038/nrmicro905
3. [3]
  (3) Muller, D. J.; Dufrene, Y. F. Nat. Nanotechnol. 2008, 3, 261. doi: 10.1038/nnano.2008.100
4. [4]
  (4) Lee, S.; Mandic, J.; Vliet, K. J. V. Proc. Natl. Acad. Sci. U. S. A. 2007, 104, 9609. doi: 10.1073/pnas.0702668104
5. [5]
  (5) Cross, S. E.; Jin, Y. S.; Rao, J.; Gimzewski, J. K. Nat. Nanotechnol. 2007, 2, 780. doi: 10.1038/nnano.2007.388
6. [6]
  (6) Neuman, K. C.; Nagy, A. Nature Methods 2008, 5, 491. doi: 10.1038/nmeth.1218
7. [7]
  (7) Stewart, M. P.; Helenius, J.; Toyoda, Y.; Ramanathan, S. P.; Muller, D. J.; Hyman, A. A. Nature 2011, 469, 226. doi: 10.1038/nature09642
8. [8]
  (8) Uchihashi, T.; Iino, R.; Ando, T.; Noji, H. Science 2011, 333, 755. doi: 10.1126/science.1205510
9. [9]
  (9) Butt, H. J.; Wolff, E. K.; uld, S. A. C.; Northern, B. D.; Peterson, C. M.; Hansma, P. K. J. Struct. Biol. 1990, 105, 54. doi: 10.1016/1047-8477(90)90098-W
10. [10]
  (10) Chen, P. P.; Dong, H. T.; Chen, L.; Sun, Q. M.; Han, D. Chin. Sci. Bull. 2009, 54, 2410. doi: 10.1007/s11434-009-0374-1
11. [11]
  (11) Wu, Y.; Lu, H.; Cai, J.; He, X.; Hu, Y.; Zhao, H.; Wang, X. Nanoscale Res. Lett. 2009, 4, 942. doi: 10.1007/s11671-009-9340-8
12. [12]
  (12) Puntheeranurak, T.; Wildling, L.; Gruber, H. J.; Kinne, R. K. H.; Hinterdorfer, P. J. Cell Sci. 2006, 119, 2960. doi: 10.1242/jcs.03035
13. [13]
  (13) Kirat, K. E.; Burton, I.; Dupres, V.; Dufrene, Y. F. J. Microsc. 2005, 218, 199. doi: 10.1111/j.1365-2818.2005.01480.x
14. [14]
  (14) Lam, W. A.; Rosenbluth, M. J.; Fletcher, D. A. Blood 2007, 109, 3505. doi: 10.1182/blood-2006-08-043570
15. [15]
  (15) Ng, L.; Hung, H. H.; Sprunt, A.; Chubinskaya, S.; Ortiz, C.; Grodzinsky, A. J. Biomech. 2007, 40, 1011. doi: 10.1016/j.jbiomech.2006.04.004
16. [16]
  (16) Park, S.; Koch, D.; Cardenas, R.; Kas, J.; Shih, C. K. Biophys. J. 2005, 89, 4330. doi: 10.1529/biophysj.104.053462
17. [17]
  (17) Martens, J. C.; Radmacher, M. Pflugers Archiv-European Journal of Physiology 2008, 456, 95. doi: 10.1007/s00424-007-0419-8
18. [18]
  (18) Lulevich, V.; Yang, H.; Isseroff, R. R.; Liu, G. Ultramicroscopy 2010, 110, 1435. doi: 10.1016/j.ultramic.2010.07.009
19. [19]
  (19) Cai, X.; Yang, X.; Cai, J.; Wu, S.; Chen, Q. J. Phys. Chem. B 2010, 114, 3833. doi: 10.1021/jp910682q
20. [20]
  (20) Kirmizis, D.; Lo thetidis, S. Int. J. Nanomed. 2010, 5, 137.
21. [21]
  (21) Leporatti, S.; Gerth, A.; Kohler, G.; Kohlstrunk, B.; Hauschildt, S.; Donath, E. FEBS Lett. 2006, 580, 450. doi: 10.1016/j.febslet.2005.12.037
22. [22]
  (22) Deng, Z.; Lulevich, V.; Liu, F.; Liu, G. J. Phys. Chem. B 2010, 114, 5971. doi: 10.1021/jp9114546
23. [23]
  (23) Muller, D. J.; Dufrene, Y. F. Trends Cell Biol. 2011, 21, 461. doi: 10.1016/j.tcb.2011.04.008
24. [24]
  (24) Muller, D. J.; Dufrene, Y. F. Curr. Biol. 2011, 21, R212.
25. [25]
  (25) Li, M.; Liu, L. Q.; Xi, N.; Wang, Y. C.; Dong, Z. L.; Xiao, X. B.; Zhang, W. J. Chin. Sci. Bull. 2010, 55, 2188. [李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 科学通报, 2010, 55, 2188.] doi: 10.1360/972010-218
26. [26]
  (26) Touhami, A.; Nysten, B.; Dufrene, Y. F. Langmuir 2003, 19, 4539. doi: 10.1021/la034136x
27. [27]
  (27) Cartron, G.; Watier, H.; lay, J.; Solal-Celigny, P. Blood 2004, 104, 2635. doi: 10.1182/blood-2004-03-1110
28. [28]
  (28) Bonavida, B. Oncogene 2007, 26, 3629. doi: 10.1038/sj.onc.1210365
29. [29]
  (29) Smith, M. R. Oncogene 2003, 22, 7359. doi: 10.1038/sj.onc.1206939
30. [30]
  (30) Hu, M.; Wang, J.; Zhao, H.; Dong, S.; Cai, J. J. Biomech. 2009, 42, 1513. doi: 10.1016/j.jbiomech.2009.03.051
31. [31]
  (31) Li, M.; Liu, L.; Xi, N.; Wang, Y.; Dong, Z.; Tabata, O.; Xiao, X.; Zhang, W. Biochem. Biophys. Res. Commu. 2011, 404, 689. doi: 10.1016/j.bbrc.2010.12.043
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Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy

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通讯作者: 陈斌, bchen63@163.com