Citation: LI Mi, LIU Lian-Qing, XI Ning, WANG Yue-Chao, DONG Zai-Li, XIAO Xiu-Bin, ZHANG Wei-Jing. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy[J]. Acta Physico-Chimica Sinica, ;2012, 28(06): 1502-1508. doi: 10.3866/PKU.WHXB201203201 shu

Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy

LI Mi ^1,2 ,
LIU Lian-Qing ^1, ,
XI Ning ^1,3, ,
WANG Yue-Chao ¹ ,
DONG Zai-Li ¹ ,
XIAO Xiu-Bin ⁴ ,
ZHANG Wei-Jing ^4,

1.
1. State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, P. R. China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
3. Department of Electrical and Computer Engineering, Michigan State University, East Lansing 48824, USA;
4. Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, P. R. China

Received Date: 9 March 2012
Available Online: 20 March 2012
Fund Project: 国家自然科学基金(60904095, 61175103) (60904095, 61175103) 国家高技术研究发展计划项目(863) (2009AA03Z316) (863) (2009AA03Z316)

Atomic force microscopy (AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions. However, owing to the lack of adequate cellular immobilization methods, AFM imaging of living, suspended mammalian cells is still a big challenge. In this paper, a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine. In this way, the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically. When the cell is stimulated by 0.2 mg·mL^-1 Rituximab for 2 h, the cell topography becomes more corrugated and Young's modulus decreases from 196 to 183 kPa. When the cell is stimulated by 0.5 mg·mL^-1 Rituximab for 2 h, the cell topography changes more significantly and some tubercles appear, and Young's modulus decreases from 234 to 175 kPa. These results thus provide a unique insight into the effects of Rituximab on individual cells.
- Atomic force microscopy,
- Lymphoma,
- Elasticity,
- Force curve,
- Young's modulus
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