Citation: ZHANG Zhe, QI Zhi-Mei, ZHANG Rong-Jun. Direct Electrochemistry of Cytochrome c Adlayer on the ITO Electrode[J]. Acta Physico-Chimica Sinica, ;2012, 28(05): 1163-1168. doi: 10.3866/PKU.WHXB201202241 shu

Direct Electrochemistry of Cytochrome c Adlayer on the ITO Electrode

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received Date: 2 December 2011
Available Online: 24 February 2012
Fund Project: 国家重点基础研究发展规划项目(973) (2009CB320300) (973) (2009CB320300) 国家自然科学基金(60978042, 61078039) (60978042, 61078039)

The electrochemical redox reaction of a cytochrome c (Cyt c) adlayer on an indium tin oxide (ITO) electrode was directly monitored and the surface concentrations of Cyt c versus solution concentrations were obtained from cyclic voltammograms. The results indicate that the surface concentration increases from 0.35 × 10^-12 to 1.53 × 10^-12 mol·cm^-2 when the solution concentration is increased from 2 to 10 μmol ·L^-1. A quasi-linear relationship between the reciprocals of surface concentration and solution concentration was observed, indicating that Cyt c adsorption on the ITO electrode closely obeys the Langmuir isothermal adsorption model. The cyclic voltammograms of the Cyt c solutions with the ITO electrode reveal that both adsorbed and dissociated Cyt c molecules are involved in the electrode reaction and that the contribution of dissociated molecules is much larger than that of adsorbed ones. The electrode reaction is basically diffusion controlled and quasi-reversible. Based on the Nicholson method, the average standard heterogeneous rate constant was determined to be 1.65×10^-3 cm· s^-1. The electrochemical activity of the Cyt c adlayer was partially lost when it was kept at 25 °C for 1 h, and was completely lost at 80 °C. The denatured Cyt c adlayer on a ld electrode can effectively inhibit the electrode reaction of K₃Fe(CN)₆ solution.
- Cytochrome c adlayer,
- Direct electrochemistry,
- Langmuir isothermal adsorption,
- Surface concentration
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沈阳化工大学材料科学与工程学院沈阳 110142