Citation: WANG Yuan-You, HUA Wei, LIU Tian-Qing. Structural Property of Hemoglobin in Span 80/PEG 400/H₂O Niosome System[J]. Acta Physico-Chimica Sinica, ;2011, 27(08): 1907-1912. doi: 10.3866/PKU.WHXB20110822 shu

Structural Property of Hemoglobin in Span 80/PEG 400/H₂O Niosome System

1.
1. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu Province, P. R. China;
2. Department of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, Jiangsu Province, P. R. China

Received Date: 28 February 2011
Available Online: 21 June 2011
Fund Project: 国家自然科学基金(20573091)资助项目 (20573091)

The structural property of hemoglobin (Hb) was studied in detail by UV-Vis absorption, fluorescence, circular dichroism, negative staining-transmission electron microscopy (NS-TEM), and freeze etching-transmission electron microscopy (FE-TEM) techniques using a Span 80/PEG 400/H₂O niosome system. The obtained results show that Hb is adsorbed onto the surface of niosome. The peptide chain of Hb spreads out and the apparent radius of the niosome, the intensities of the UV-Vis absorption peaks, and the fluorescence peaks increase. For the second structural parameter of Hb, the α-helix content decreases but the β-sheet and β-turn content increases. The stability of Hb varies with that of the niosome.
- Hemoglobin,
- Structure,
- Property,
- Niosome
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沈阳化工大学材料科学与工程学院沈阳 110142

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通讯作者: 陈斌, bchen63@163.com

Structural Property of Hemoglobin in Span 80/PEG 400/H₂O Niosome System