Citation: ZENG Chi, ZHU Jian-Yu. Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu[J]. Acta Physico-Chimica Sinica, ;2011, 27(06): 1525-1530. doi: 10.3866/PKU.WHXB20110618 shu

Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu

ZENG Chi ^1, ,
ZHU Jian-Yu ^2,

1.
1. School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China;
2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, P. R. China

Received Date: 16 February 2011
Available Online: 29 April 2011
Fund Project: 国家自然科学基金(31000050, 30700008) (31000050, 30700008)湖北省教育厅科学技术研究项目(B20111701)资助 (B20111701)

Microcalorimetry was used to study the influence of extracellular NaCl concentration on the growth metabolism of Halobacterium halobium (Hbt. halobium) using a TAM air isothermal microcalorimeter. The metabolic thermogenic curves of Hbt. halobium growth in different concentrations of NaCl were obtained using the ampoule method. The thermokinetic equations and parameters of Hbt. halobium growth were calculated and the relationship between each thermokinetic parameter and the concentration of NaCl was obtained. The results showed that the optimum NaCl concentration for Hbt. halobium growth was not a wide range from 3.5 mol L^-1 to about 5.2 mol·L^-1 (NaCl saturation), as is generally acknowledged, but just around 3.9 mol·L^-1. For an extracellular NaCl concentration above 3.9 mol·L^-1, the growth metabolism of Hbt. halobium decreased constantly with an increase in the NaCl concentration. Further investigation by transmission electron microscopy revealed that the Hbt. halobium cells growing in approaching NaCl saturation underwent plasmolysis, which interpreted the finding of microcalorimetry perfectly. All these results led to a new interpretation of the structural transformations of Hbt. halobium upon NaCl concentration altering.
- Microcalorimetry,
- Haloarchaea,
- Extracellular NaCl concentration,
- Cell surface structure
1. [1]
  
  (1) Oren, A. The Order Halobacteriales. In The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 3rd ed.; Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H., Stackebrandt, E. Eds.; Springer-Verlag: New York, 1999.
2. [2]
  (2) Grant,W. D.; Kamekura, M.; McGenity, T. J.; Ventosa, A. Halobacteria. In Bergey’ s Manual of Systematic Bacteriology, 2nd ed.; Garrity, G. M. Eds.; Springer-Verlag: New York, 2001.
3. [3]
  (3) Stoeckenius,W.; Rowen, R. J. Cell Biol. 1967, 34, 365.
4. [4]
  (4) Grant,W. D. Phil. Trans. R. Soc. Lond. B 2004, 359, 1249.
5. [5]
  (5) Willey, J.; Sherwood, L.;Woolverton, C. Prescott′s Microbiology, 8th ed.; McGraw-Hill: New York, 2011; chap. 6.
6. [6]
  (6) Zeng, C.; Zhu, J. C.; Liu, Y.; Yang, Y.; Zhu, J. Y.; Huang, Y. P.; Shen, P. J. Therm. Anal. Cal. 2006, 84, 625.
7. [7]
  (7) Wads?, I. Thermochim. Acta 2002, 394, 305.
8. [8]
  (8) Braissant, O.;Wirz, D.; G?pfert, B.; Daniels, A. U. FEMS Microbiol. Lett. 2010, 303, 1.
9. [9]
  (9) Rodriguez-Valera, F.; Ruiz-Berraquero, F.; Ramos-Cormenzana, A. J. Gen. Microbiol. 1980, 119, 535.
10. [10]
  (10) Gibbons, N. E.; Payne, J. I. Can. J. Microbiol. 1961, 7, 483.
11. [11]
  (11) Pritchard, J. Tur r Pressure. In Encyclopedia of Life Sciences; Nature Publishing Group: London, 2001.
12. [12]
  (12) Koch, A. L. Growth Measurement. In Manual of Methods for General Bacteriology; Gerhardt, P. Eds.; American Society for Microbiology:Washington D.C., 1981; chap. 11.
13. [13]
  (13) Shand, R. F.; Perez, A. M. Haloarchaeal Growth Physiology. In Enigmatic Organisms and Life in Extreme Environments; Seckback, J. Eds.; Kluwer Academic Publishers: Dordrecht, 1999; pp 413-424.
14. [14]
  (14) Dyall-Smith, M. The Halohandbook: Protocols for Haloarchaeal Genetics, version 7.1; 2009; pp 109-111. http://www.haloarchaea.com/resources/halohandbook
15. [15]
  (15) Huang, Y. P.; Liu, Y.; Shen, P.; Qu, S. S. J. Therm. Anal. Cal. 2003, 74, 163.
16. [16]
  (16) Liu, P.; Liu, Y.; Xie, Z. X.; Chen, Y. G.; Zhao, R. M.; Shen, P.; Qu, S. S. Chin. J. Chem. 2003, 21, 693.
17. [17]
  [刘鹏, 刘义, 谢之雄, 陈酉贵, 赵儒铭, 沈萍, 屈松生. 中国化学, 2003, 21, 693.]
18. [18]
  (17) Liu, Y.; Yu, H. G.; Huang, Y. P.; Shen, P.; Qu, S. S. Biol. Trace Elem. Res. 2003, 92, 83.
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