Citation: HAO Ping, GAO Yun-Yan, OU Zhi-Ze, LI Yi, WANG Zhong-Li, WANG Xue-Song. Preparation of Aminopyridine Grafted Carbon Nanotube and Its Interaction with Horseradish Peroxidase[J]. Acta Physico-Chimica Sinica, ;2011, 27(01): 233-240. doi: 10.3866/PKU.WHXB20110125 shu

Preparation of Aminopyridine Grafted Carbon Nanotube and Its Interaction with Horseradish Peroxidase

HAO Ping ¹ ,
GAO Yun-Yan ^1, ,
OU Zhi-Ze ^1, ,
LI Yi ² ,
WANG Zhong-Li ¹ ,
WANG Xue-Song ²

1.
1. Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi′an 710072, P. R. China;
2. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry,Chinese Academy of Sciences, Beijing 100190, P. R. China

Received Date: 17 August 2010
Available Online: 8 December 2010
Fund Project: 教育部留学回国人员科研启动基金(N9YK0003, N9YK0005) (N9YK0003, N9YK0005) 西北工业大学基础研究基金(NPU-FFR-JC200822) (NPU-FFR-JC200822)西北工业大学翱翔之星项目(07XE0152)资助 (07XE0152)

Carboxylic-functionalized multiwalled carbon nanotube (MWCNT-COOH) is obtained by oxidation with potassium bichromate and further modification by amide condensation afforded aminopyridine-grafted MWCNT (MWCNT-AP). The MWCNT-AP was characterized by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (¹H-NMR) and X-ray photoelectron spectroscopy (XPS). Transmission electron microscopy (TEM) results suggest that MWCNTCOOH aggregates in ethanol and that MWCNT-AP is stable and well dispersed in solution. Horseradish peroxidase (HRP) physically adsorbed onto the surfaces of MWCNT-AP and MWCNT-COOH and the adsorption amounts were 187.5 and 153.0 μg·mg^-1, respectively. UV-Vis spectra showed that the Soret band of HRP red-shifted markedly after adsorption onto MWCNT-AP or MWCNT-COOH indicating that the binding site of MWCNT-AP or MWCNT-COOH is near the heme pocket of HRP. Circular dichroism spectral results demonstrate that the secondary structure of HRP is influenced by MWCNT-AP. Enzyme-kinetic studies show that MWCNT-AP may adsorb HRP and its substrate 3,3',5,5'-tetramethylbenzidine (TMB) effectively, and the maximum reaction rate (V_max) of HRP increases significantly after interaction with MWCNT-AP.
- Carbon nanotube,
- Surface functionalization,
- Horseradish peroxidase,
- Catalytic activity of enzyme,
- Hydrogen bonding
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