Citation: ZHOU Wen-Wen, LIAN Jie, HU Ke-Jia, GAO Yun-Hua, XU Bai. Effect of Surface Chemical Properties of a Silicon Chip on Antibody Immobilization[J]. Acta Physico-Chimica Sinica, ;2010, 26(10): 2821-2827. doi: 10.3866/PKU.WHXB20101016 shu

Effect of Surface Chemical Properties of a Silicon Chip on Antibody Immobilization

ZHOU Wen-Wen ^1,2 ,
LIAN Jie ^1,2 ,
HU Ke-Jia ^1,2 ,
GAO Yun-Hua ^1, ,
XU Bai ^1,

1.
1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry,Chinese Academy of Sciences, Beijing 100190, P. R. China;
2. Graduate University of Chinese Academy of Sciences,Beijing 100049, P. R. China

Received Date: 22 April 2010
Available Online: 27 September 2010
Fund Project: 国家自然科学基金(20975106) (20975106) 中国科学院知识创新工程重要方向项目(KJCX2-YW-M15) 及国家重大专项(2008ZX08012-001) 资助 (KJCX2-YW-M15) 及国家重大专项(2008ZX08012-001)

The key to constructing a protein microarray is the stable immobilization of proteins and the retention of their biological activities. In this study, immobilization of the carcinoembryonic antigen (CEA) antibody onto a silicon dioxide surface was investigated by physical adsorption, direct chemical covalent conjugation, spacer-added chemical covalent conjugation, and biological affinity interactions. Based on the specific antibody-antigen interactions, the sandwich reaction, enzyme-linked immunosorbent assay (ELISA) was chosen to evaluate various immobilization strategies. The most efficient immobilization strategy was with glutaraldehyde as a coupling reagent between the CEA antibody and the amino surface. The attachment of a spacer-armcomprising poly-L-lysine significantly improved the immobilization efficiency and simultaneously decreased nonspecific adsorption. High immobilization efficiency and stronger nonspecific adsorption were also observed when the CEA antibody was immobilized by bioaffinity interactions.
- Protein microarray,
- Antibody immobilization,
- Sandwich reaction,
- Enzyme-linked immunosorbent assay,
- Amino surface,
- Glutaraldehyde,
- Poly-L-lysine
1. [1]
  
  1. Zhu, H.; Snyder, M. Curr. Opin. Chem. Biol., 2001, 5: 40
2. [2]
  2. MacBeath, G. Nat Genet, 2002, 32(suppl.): 526
3. [3]
  3. Templin, M. F.; Stoll, D.; Schrenk, M.; Traub, P. C.; Võhringer, C. F.; Joos, T. O. Trends Biotechnol., 2002, 20: 160
4. [4]
  4. Ng, J. H.; Ilag, L. L. Journal of Cellular and Molecular Medicine, 2002, 6: 329
5. [5]
  5. Wilson, D. S.; Nock, S. Angew. Chem. Int. Edit., 2003, 42: 494
6. [6]
  6. Angenendt, P. Drug Discovery Today, 2005, 10: 503
7. [7]
  7. Kusnezow, W.; Hoheisel, J. D. Biotechniques, 2002, 33(suppl.): 14
8. [8]
  8. Liotta, L. A.; Espina, V.; Mehta, A. I.; Calvert, V.; Rosenblatt, K.; Geho, D.; Munson, P. J.; Young, L.; Wulfkuhle, J.; Petricoin III, E. F. Cancer Cell, 2003, 3: 317
9. [9]
  9. Lutanie, E.; Voegel, J. C.; Schaaf, P.; Freund, M.; Cazenave, J. P.; Schmitt, A. Proc. Natl. Acad. Sci. U. S. A., 1992, 89: 9890
10. [10]
  10. Vijayendran, R. A.; Leckband, D. E. Anal. Chem., 2000, 73: 471
11. [11]
  11. Rossier, J. S.; kulrangan, G.; Girault, H. H.; Svojanovsky, S.; Wilson, G. S. Langmuir, 2000, 16: 8489
12. [12]
  12. Angenendt, P.; Glõkler, J.; Murphy, D.; Lehrach, H.; Cahill, D. J. Anal. Biochem., 2002, 309: 253
13. [13]
  13. Kusnezow, W.; Hoheisel, J. D. J. Mol. Recognit., 2003, 16: 165
14. [14]
  14. Seong, S. Y. Clin Diagn Lab Immunol, 2002, 9: 927
15. [15]
  15. Kusnezow, W.; Jacob, A.; Walijew, A.; Diehl, F.; Hoheisel, J. D. Proteomics, 2003, 3: 254
16. [16]
  16. Niemeyer, C. M.;Wacker, R.; Adler, M. Nucl. Acids Res., 2003, 31: e90
17. [17]
  17. Peluso, P.;Wilson, D. S.; Do, D.; Tran, H.; Venkatasubbaiah, M.; Quincy, D.; Heidecker, B.; Poindexter, K.; Tolani, N.; Phelan, M.; Witte, K.; Jung, L. S.;Wagner, P.; Nock, S. Anal. Biochem., 2003, 312: 113
18. [18]
  18. Ramachandran, N.; Hainsworth, E.; Bhullar, B.; Eisenstein, S.; Rosen, B.; Lau, A. Y.;Walter, J. C.; LaBaer, J. Science, 2004, 305: 86
19. [19]
  19. Oh, S. J.; Hong, B. J.; Choi, K. Y.; Park, J. W. OMICS, 2006, 10: 327
20. [20]
  20. Iwata, R.; Satoh, R.; Iwasaki, Y.; Akiyoshi, K. Colloids Surf. B, 2008, 62: 288
21. [21]
  21. Hermanson, G. T. Bioconjugate techniques. 2nd ed. Rockford, Illinois: Academic Press, 2008
22. [22]
  22. Haab, B. B.; Dunham, M. J.; Brown, P. O. Genome Biol., 2001, 2: 0004.1
23. [23]
  23. Stillman, B. A.; Tonkinson, J. L. Biotechniques, 2000, 29: 630
24. [24]
  24. Qian,W.; Yao, D.; Yu, F.; Xu, B.; Zhou, R.; Bao, X.; Lu, Z. Clin. Chem., 2000, 46: 1456
25. [25]
  25. MacBeath, G.; Schreiber, S. L. Science, 2000, 289: 1760
26. [26]
  26. Piehler, J.; Brecht, A.; Geckeler, K. E.; Gauglitz, G. Biosens. Bioelectron., 1996, 11: 579
27. [27]
  27. Penzol, G.; Armisén, P.; Fernández-Lafuente, R.; Rodés, L.; Guisán, J. M. Biotechnol. Bioeng., 1998, 60: 518
28. [28]
  28. Afanassiev, V.; Hanemann, V.;Wolfl, S. Nucl. Acids Res., 2000, 28: e66
29. [29]
  29. Piletsky, S. A.; Matuschewski, H.; Schedler, U.; Wilpert, A.; Piletska, E. V.; Thiele, T. A.; Ulbricht, M. Macromolecules, 2000, 33: 3092
30. [30]
  30. Wilson, D. S.; Nock, S. Curr. Opin. Chem. Biol., 2002, 6: 81
31. [31]
  31. Neubert, H.; Jacoby, E. S.; Bansal, S. S.; Iles, R. K.; Cowan, D. A.; Kicman, A. T. Anal. Chem., 2002, 74: 3677
32. [32]
  32. Gupta, B.; Plummer, C.; Bisson, I.; Frey, P.; Hilborn, J. Biomaterials, 2002, 23: 863
33. [33]
  33. Stine, R.; Cole, C. L.; Ainslie, K. M.; Mulvaney, S. P.;Whitman, L. J. Langmuir, 2007, 23: 4400
34. [34]
  34. Gibson, T. D.;Woodward, J. R. Protein stabilization in biosensor systems//Biosensors and chemical sensors.Washington, DC: American Chemical Society, 1992: 40
35. [35]
  35. Mateo, C.; Torres, R.; Fernández-Lorente, G.; Ortiz, C.; Fuentes, M.; Hidal , A.; López-Galle , F.; Abian, O.; Palomo, J. M.; Betancor, L.; Pessela, B. C. C.; Guisan, J. M.; Fernández-Lafuente, R. Biomacromolecules, 2003, 4: 772
36. [36]
  36. Lee, S. D.; Hsiue, G. H.; Chang, P. C. T.; Kao, C. Y. Biomaterials, 1996, 17: 1599
37. [37]
  37. Kato, K.; Ikada, Y. Biotechnol. Bioeng., 1995, 47: 557
38. [38]
  38. Lee,W.; Oh, B. K.; Bae, Y. M.; Paek, S. H.; Lee, W. H.; Choi, J. W. Biosensors and Bioelectronics, 2003, 19: 185
39. [39]
  39. Turková, J. J. Chromatogr. B, 1999, 722: 11
40. [40]
  40. Jung, Y.; Lee, J. M.; Jung, H.; Chung, B. H. Anal. Chem., 2007, 79: 6534
41. [41]
  41. Võlimaa, L. Streptavidin———a versatile binding protein for solid- phase immunoassays [D]. Turku: University of Turku, 2008
42. [42]
  42. Lue, R. Y. P.; Chen, G. Y. J.; Zhu, Q.; Lesaicherre, M. -L.; Yao, S. Q. Site-specific immobilization of biotinylated proteins for protein microarray analysis//Protein arrays. Totowa: Humana Press Inc., 2004: 85
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