Citation: SHAO Shuang, HU Xiao-Huan, YOU Jin-Zong, LEI Qun-Fang. Interactions between N-Alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium Bromide and Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2010, 26(11): 2997-3001. doi: 10.3866/PKU.WHXB20101008 shu

Interactions between N-Alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium Bromide and Bovine Serum Albumin

1.
1. Department of Chemistry, Zhejiang International Studies University, Hangzhou 310012, P. R. China;
2. Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China

Received Date: 13 April 2010
Available Online: 25 August 2010
Fund Project: 国家自然科学基金(20673098) (20673098)浙江省自然科学基金(Y4080201)资助项目 (Y4080201)

Interactions between a series of N-alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium bromides with alkyl chain from dodecyl to cetyl and bovine serum albumin (BSA) in Tris-HCl buffer solution (pH=7.1) were studied by fluorescence spectroscopy at 298 K. Effects of the surfactant structure and BSA concentration on the interactions were examined. The interaction mechanism between BSA and surfactants at a relatively low concentration range was discussed using the Stern-Volmer equation and a model of the pseudo association constant. Each of the quaternary ammonium surfactants has a fluorescence quenching effect on BSA and leads to a blue shift of the maximum emission wavelength. The Stern-Volmer quenching constant and the pseudo association constant increase, which indicates that the interactions between surfactants and BSA become stronger, as the hydrophobic chain lengths increase.
- Quaternary ammoniumsurfactant,
- Bovine serumalbumin,
- Fluorescence quenching,
- Hydrophobic interaction,
- Pseudo association constant
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