Citation: DENG Lin, QI Zhi-Mei. Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy[J]. Acta Physico-Chimica Sinica, ;2010, 26(10): 2672-2678. doi: 10.3866/PKU.WHXB20101003 shu

Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy

DENG Lin ,
QI Zhi-Mei

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received Date: 24 May 2010
Available Online: 27 September 2010
Fund Project: 国家重点基础研究发展计划(973)(2009CB320300) (973)(2009CB320300)国家自然科学基金(60978042) (60978042)

We used time-resolved optical waveguide spectroscopy (OWGS) to in-situ measure visible absorption spectrum for methylene blue (MB) adsorbed ona hydrophilic glass waveguide from an aqueous solution containing proteins such as bovine serum albumin (BSA) and hemoglobin (Hb). The competitive adsorption of MB and the protein was detected at a solution pH lower than the isoelectric point of the protein. Since the competitive adsorption behavior of MB and the protein is very sensitive to the protein concentration, the protein content in the mixed solution was readily determined by time-resolved OWGS. In addition, a kinetic equation for the competitive adsorption of the two molecules was deduced based on the Langmuir adsorption isotherm. The best fit of the measured time course of the absorbance with the theoretical kinetic equation reveals that the number of MB molecules adsorbed on the surface exponentially decreases with time after reaching a maximum.A quasi-linear relationship between the fitting parameters and the protein concentration was also obtained.
- Optical waveguide spectroscopy,
- Competitive adsorption,
- Methylene blue,
- Protein detection
1. [1]
  
  1. Marco, A.; Rubio, R.; Compano, R.; Casals, I. Talanta, 2002, 57: 1019
2. [2]
  2. Coakley, W. T.; James, C. J. Anal. Biochem., 1978, 85: 90
3. [3]
  3. Shen, W.; Chen, J. Z.; Dai, C. J. Food Res. Dev., 2009, 30: 96 [沈文,陈均志,代春吉. 食品研究与开发, 2009, 30: 96]
4. [4]
  4. Li, J.; Zhang, Y. T.; Zeng, W.; Luo, X.; Liao, C. C. Chin. J. Biol., 2000, 13: 118 [李娟,张耀庭,曾伟,罗璇,廖长春.中国生物制品学杂志, 2000, 13: 118]
5. [5]
  5. Minagawa, M.; Winter, D. A.; Kaplan, I. R. Anal. Chem., 1984, 56: 1859
6. [6]
  6. Penninckx,W.; Verbeke, J. S.; Vankeerberghen, P.; Massart, D. L. Anal. Chem., 1996, 68: 481
7. [7]
  7. Yu, X. L.; Wei, X.; Wang, D. X.; Ding, X.; Liao, W.; Zhao, X. S. Acta Phys.-Chim. Sin., 2005, 21: 888 [余兴龙,魏星,王鼎新, 定翔,廖伟,赵新生.物理化学学报, 2005, 21: 888]
8. [8]
  8. Schmitt, K.; Schirmer, B.; Hoffmann, C.; Brandenburg, A.; Meyrueis, P. Biosens. Bioelectro., 2007, 22: 2591
9. [9]
  9. Wu, Z.W.; Liu, Q. J.; Wu, L. W.; Xie, X.; Zhuang, Y.;Wen, T.; Bai, Y. F.; Lu, Z. H. Acta Optica Sinica, 2007, 27: 521 [吴中伟, 刘全俊,吴凌伟, 谢骁,庄萤,温恬, 白云飞, 陆祖宏.光学学报, 2007, 27: 521]
10. [10]
  10. Kim, N.; Kim, D. K.; Kim, W. Y. Food Chem., 2008, 108: 768
11. [11]
  11. Enami, Y.; Fukuda, T.; Suye, S. Appl. Phys. Lett., 2007, 91: 203507-1
12. [12]
  12. Kim, N.; Kim, D. Y.; Cho, Y. J.; Moon, D. K.; Kim, W. Y. Biosens. Bioelectro., 2008, 24: 391
13. [13]
  13. Choquette, S. J.; Browm, L. L.; Durst, R. A. Anal. Chem., 1992, 64: 55
14. [14]
  14. Bradshaw, J. T.; Mendes, S. B.; Saavedra, S. S. Anal. Chem., 2005: 29A
15. [15]
  15. Qi, Z. M.; Matsuda, N.; Takatsu, A.; Kato, K. J. Phys. Chem. B, 2003, 107: 6873
16. [16]
  16. Cameron, P. J.; Jenkins, A. T. A.; Knoll, W.; Marken, F.; Milsom, E. V.; Williams, T. L. J. Mater. Chem., 2008, 18: 4304
17. [17]
  17. Qi, Z. M.; Xia, S. H.; Matsuda, N. Anal. Biochem., 2008, 374: 196
18. [18]
  18. Mendes, S. B.; Saavedra, S. S. Opt. Express, 1999, 4: 449
19. [19]
  19. Fujita, K.; Taniguchi, K.; Ohno, H. Talanta, 2005, 65: 1066
20. [20]
  20. Deng, L.; Lu, D. F.; Qi, Z. M. Acta Phys.-Chim. Sin., 2009, 25: 2481 [邓琳,逯丹凤,祁志美. 物理化学学报, 2009, 25: 2481]
21. [21]
  21. Azizian, S.; Haerifar, M.; Parsa, J. B. Chemosphere, 2007, 68: 2040
22. [22]
  22. mri, S.; Seguin, J. L.; Guerin, J.; Aguir, K. Sens. Actuators, 2006, 114: 451
23. [23]
  23. Koutsopoulos, S.; Patzsch, K.; Bosker,W. T. E.; Norde, W. Langmuir, 2007, 23: 2000
24. [24]
  24. Kusakari, A.; Izumi, M.; Ohnuki, H. Colloids Surf. A, 2008, 321: 47
25. [25]
  25. Can, O.; Holland, N. B. J. Colloid Interface Sci., 2009, 329: 24
1. [1]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
2. [2]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
3. [3]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
4. [4]
  Yan Yuan , Haitao Wu , Yi Zhang , Li Jiang , Feng Cao , Yanmao Dong . Research on the Talent Training System to Enhance the Core Competence of Employment for Undergraduate Students Majoring in Materials Chemistry. University Chemistry, 2024, 39(11): 52-56. doi: 10.12461/PKU.DXHX202402015
5. [5]
  Xiaosong PU , Hangkai WU , Taohong LI , Huijuan LI , Shouqing LIU , Yuanbo HUANG , Xuemei LI . Adsorption performance and removal mechanism of Cd(Ⅱ) in water by magnesium modified carbon foam. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1537-1548. doi: 10.11862/CJIC.20240030
6. [6]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
7. [7]
  Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
8. [8]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
9. [9]
  Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351
10. [10]
  Xiaoning TANG , Junnan LIU , Xingfu YANG , Jie LEI , Qiuyang LUO , Shu XIA , An XUE . Effect of sodium alginate-sodium carboxymethylcellulose gel layer on the stability of Zn anodes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1452-1460. doi: 10.11862/CJIC.20240191
11. [11]
  Chi Li , Jichao Wan , Qiyu Long , Hui Lv , Ying Xiong . N-Heterocyclic Carbene (NHC)-Catalyzed Amidation of Aldehydes with Nitroso Compounds. University Chemistry, 2024, 39(5): 388-395. doi: 10.3866/PKU.DXHX202312016
12. [12]
  Fei Xie , Chengcheng Yuan , Haiyan Tan , Alireza Z. Moshfegh , Bicheng Zhu , Jiaguo Yu . d带中心调控过渡金属单原子负载COF吸附O₂的理论计算研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2407013-. doi: 10.3866/PKU.WHXB202407013
13. [13]
  Xinhao Yan , Guoliang Hu , Ruixi Chen , Hongyu Liu , Qizhi Yao , Jiao Li , Lingling Li . Polyethylene Glycol-Ammonium Sulfate-Nitroso R Salt System for the Separation of Cobalt (II). University Chemistry, 2024, 39(6): 287-294. doi: 10.3866/PKU.DXHX202310073
14. [14]
  Jie ZHAO , Sen LIU , Qikang YIN , Xiaoqing LU , Zhaojie WANG . Theoretical calculation of selective adsorption and separation of CO₂ by alkali metal modified naphthalene/naphthalenediyne. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 515-522. doi: 10.11862/CJIC.20230385
15. [15]
  Youlin SI , Shuquan SUN , Junsong YANG , Zijun BIE , Yan CHEN , Li LUO . Synthesis and adsorption properties of Zn(Ⅱ) metal-organic framework based on 3, 3', 5, 5'-tetraimidazolyl biphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1755-1762. doi: 10.11862/CJIC.20240061
16. [16]
  Qianqian Zhong , Yucui Hao , Guotao Yu , Lijuan Zhao , Jingfu Wang , Jian Liu , Xiaohua Ren . Comprehensive Experimental Design for the Preparation of the Magnetic Adsorbent Based on Enteromorpha Prolifera and Its Utilization in the Purification of Heavy Metal Ions Wastewater. University Chemistry, 2024, 39(8): 184-190. doi: 10.3866/PKU.DXHX202312013
17. [17]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
18. [18]
  Xinyu Yin , Haiyang Shi , Yu Wang , Xuefei Wang , Ping Wang , Huogen Yu . Spontaneously Improved Adsorption of H₂O and Its Intermediates on Electron-Deficient Mn^(3+δ)+ for Efficient Photocatalytic H₂O₂ Production. Acta Physico-Chimica Sinica, 2024, 40(10): 2312007-. doi: 10.3866/PKU.WHXB202312007
19. [19]
  Shasha Ma , Zujin Yang , Jianyong Zhang . Facile Synthesis of FeBTC Metal-Organic Gel and Its Adsorption of Cr₂O₇²⁻: A Physical Chemistry Innovation Experiment. University Chemistry, 2024, 39(8): 314-323. doi: 10.3866/PKU.DXHX202401008
20. [20]
  Junli Liu . Practice and Exploration of Research-Oriented Classroom Teaching in the Integration of Science and Education: a Case Study on the Synthesis of Sub-Nanometer Metal Oxide Materials and Their Application in Battery Energy Storage. University Chemistry, 2024, 39(10): 249-254. doi: 10.12461/PKU.DXHX202404023

Get Citation

PDF

XML

Metrics

PDF Downloads(1271)
Abstract views(2920)
HTML views(32)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142