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Citation: LIU Ping, XING Guo-Wen, LI Xuan-Wen, YE Yun-Hua. Adsorptive Immobilization of Four Proteases on Different Molecular Sieves[J]. Acta Physico-Chimica Sinica, ;2010, 26(04): 1113-1118. doi: 10.3866/PKU.WHXB20100448 shu

Adsorptive Immobilization of Four Proteases on Different Molecular Sieves

1.
Beijing National Laboratory for Molecular Science, Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China

Received Date: 30 November 2009
Available Online: 17 March 2010

The immobilization of four different proteases on a series of molecular sieves, including microporous HY, NaY, NH4Y, MCM-22, Hβ zeolites, modified Y zeolites HDAY, HNH4DAY, and mesoporous MCM-41, by adsorption was systematically investigated. The four proteases were α-chymotrypsin, papain, subtilisin, and thermoase (or its pure form thermolysin). The results showed that the enzyme loading amount and the activity of immobilized enzyme were significantly affected not only by the structures and textures of molecular sieves and the enzyme properties, but also by the adsorptive conditions such as buffer pH values and enzyme concentration. In most cases, the amount of protease loading on molecular sieves was relatively higher at pH 6.0, but declined with further increasing of pH values. The nature of the interaction between protease and molecular sieves is discussed. As for α-chymotrypsin, its loading amount on Hβ zeolites was found to be the highest, whereas the activity of α-chymotrypsin immobilized on MCM-22 was the highest, which is probably due to different adsorption states.
- Adsorption,
- Immobilization,
- Protease,
- Zeolite,
- Molecular sieve,
- Peptide synthesis
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