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Citation: LIU Bao-Sheng, ZHAO Feng-Li, XUE Chun-Li, WANG Jing, LV Yun-Kai. Application of Fluorescence Spectroscopy to a Study on the Anta nism between Chloramphenicol and Sarafloxacin[J]. Acta Physico-Chimica Sinica, ;2009, 25(09): 1861-1866. doi: 10.3866/PKU.WHXB20090916 shu

Application of Fluorescence Spectroscopy to a Study on the Anta nism between Chloramphenicol and Sarafloxacin

1.
Key Laboratory of Medical Chemistry and Molecular Diagnosis, Ministry of Education, Center of Physics and Chemistry, Hebei University, Baoding 071002, Hebei Province, P. R. China

Received Date: 5 March 2009
Available Online: 16 July 2009

Both chloramphenicol (CHL) and sarafloxacin (SLFX) can quench the fluorescence from bovine serum albumin (BSA). The fluorescence will quench to a larger degree when the two drugs coexist. We thus studied the anta nism between SLFX and CHL using fluorescence spectroscopy. We prove that the anta nism between these drugs increases the binding stability between drug and protein. At the same time, a reduction of the free drug's concentration will reduce the effect of the drugs. Results show that the quenching mechanism of the combination for bovine serum albumin and drugs is a static procedure. The number of binding sites is 1. Based on the theory of Forster energy transfer spectroscopy, the binding distance r between drugs and bovine serum albumin was obtained. Because of the existence of anta nism between the drugs, the correlation coefficient and binding distance are reduced. Synchronous spectra were obtained, which showed the effect of this anta nismbetween the drugs on the conformation of BSA. The protein molecules are extended and their hydrophobic nature is reduced.
- Fluorescence spectrum,
- Sarafloxacin,
- Chloramphenicol,
- Bovine serumalbumin,
- Anta nism
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