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Citation: SHAO Shuang, QIU Jin. Effecting of Metal Ions on the Interaction between Zidovudine and Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2009, 25(07): 1342-1346. doi: 10.3866/PKU.WHXB20090706 shu

Effecting of Metal Ions on the Interaction between Zidovudine and Bovine Serum Albumin

SHAO Shuang ,
QIU Jin

1.
Department of Chemistry, Zhejiang Education Institute, Hangzhou 310012, P. R. China

Received Date: 6 January 2009
Available Online: 21 April 2009

The interaction between zidovudine (ZDV) and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and ultraviolet-visible spectroscopy in aqueous solution (Tris-HCl buffer, pH 7.1). The effect of metal ions (Cu2+, Mg2+, Zn2+) on the interaction was also investigated. Results showed that the fluorescence intensity of BSA at 346 nm was quenched when zidovudine or metal ions were added. The quenching mechanism was a static quenching mechanism. A strong interaction exists between zidovudine and BSA. The thermodynamic parameters △H and △S were -10.2 kJ·mol-1 and 77.5 J·mol-1·K-1 at 298 K, respectively, indicating that electro-static forces played a major role. The binding constant, the number of binding site and the binding distance were 6.92×105 L·mol-1, 1.18, and 2.28 nm at 298 K, respectively. The binding constant and the number of binding sites decreased with the increase in temperature. The metal ions, Cu2+, Mg2+ and Zn2+, all decreased the binding constant and increased the binding distance for binding between ZDV and BSA.
- Bovine serumalbumin,
- Zidovudine,
- Metal ion,
- Fluorescence quenching
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