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Citation: WANG Fang-Bin, PENG Yong, FAN Mei-Yi, LIU You-Nian, HUANG Ke-Long. Synthesis of Glutathionyl Ferrocene and Its Interaction with Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2009, 25(06): 1125-1130. doi: 10.3866/PKU.WHXB20090612 shu

Synthesis of Glutathionyl Ferrocene and Its Interaction with Bovine Serum Albumin

1.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China

Received Date: 19 January 2009
Available Online: 31 March 2009

Glutathionyl ferrocene (GSH-Fc) was synthesized from glutathione and ferrocenyl amine using O-(benzotriazol-yl)-N,N,N',N'-tetramethyluronium(HBTU)/1-hydroxybenzotrizole (HOBT) as coupling agents in solution with a yield of 23.2%. The compound was characterized by 1H-NMR, IR and MS. Interactions of bovine serum albumin (BSA) with glutathionyl ferrocene were investigated by electrochemical methods. Experimental results revealed that there were specific interactions between BSA and GSH-Fc at the IIA subdomain. The binding constant and binding sites were obtained by linear sweep voltammetry and had values of 1.71×106 L·mol-1 and 1.30, respectively. Fluorescence spectroscopy methods were employed to investigate the interaction between GSH-Fc and BSA as well. Results showed that the fluorescence of BSA was quenched by GSH-Fc during a static quenching process. The binding constant and the number of binding sites were obtained and had values of 2.74×106 L·mol-1 and 1.57, respectively, which agreed well with results fromelectrochemical experiments.
- Glutathionyl ferrocene,
- BSA,
- Interaction,
- Electrochemistry,
- Fluorescence spectroscopy
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