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Citation: WANG Dong-Dong, SUN De-Zhi, LI Lin-Wei, WEI Xin-Ting, ZHANG Ai-Mei. Interactions between 5-fluorouracil and Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2007, 23(10): 1627-1630. doi: 10.3866/PKU.WHXB20071026 shu

Interactions between 5-fluorouracil and Bovine Serum Albumin

1.
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, Shandong Province, P. R. China

Received Date: 25 April 2007
Available Online: 6 August 2007

From reasonable suppositions on binding process and Langmuir’s binding theory, the interactions between 5-fluorouracil (5-FU) and bovine serumalbumin (BSA) were investigated by the isothermal titration calorimetry (ITC) and the circular dichroism (CD) spectrometry at 298.15 K. The results showed that there were two classes of binding sites on a protein (BSA) molecule for the 5-fluorouracil. For the first class of binding, N=(54.0±0.3), ⊿H⁰=(30.0±0.4) kJ·mol^-1 (endothermal), ⊿S⁰=(196.0±2.6) J·mol-1·K^-1 (entropy increasing), ⊿G⁰=(-28.4±0.3) kJ·mol^-1; for the second class of binding, N=(77.0±0.4), ⊿H⁰=(-20.0±0.4) kJ·mol^-1 (exothermal), ⊿S⁰=(28.6±0.3) J·mol^-1·K^-1 (entropy increasing), ⊿G⁰=(-28.5±0.2) kJ·mol ^-1. The interactions between 5-fluorouracil and BSA induced the relative contents of secondary structure units of protein (BSA) to change in two classes of binding processes.
- 5-fluorouracil; Bovine serum albumin; Isothermal titration calorimetry; Circular dichroism spectra
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沈阳化工大学材料科学与工程学院沈阳 110142

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