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Citation: LIANG Jin-Ling, ZHOU Jian-Zhang, CHEN Qiao-Lin, LIN Ling-Ling, LIN Zhong-Hua. Effect of Interface Electric Field on DNA Hybridization Studied by Electrochemical Quartz Crystal Microbalance[J]. Acta Physico-Chimica Sinica, ;2007, 23(09): 1421-1424. doi: 10.3866/PKU.WHXB20070921 shu

Effect of Interface Electric Field on DNA Hybridization Studied by Electrochemical Quartz Crystal Microbalance

1.
State Key Laboratory for Physical Chemistry of the Solid Surface, Department of Chemistry, Xiamen University, Xiamen 361005, Fujian Province, P. R. China

Received Date: 20 April 2007
Available Online: 6 July 2007

The hybridization of oli nucleotide probe with matched or mismatched DNA target was monitored by a combination of electrochemical control and in situ quartz crystal microbalance technique. The results indicated that the apparent efficiency of hybridization for matched DNA target at a positive potential was higher than that at a negative potential. But an obvious response of “false positives”may be obtained at the positive potential. Application of negative potential can availably halt hybridization for mismatched target DNA. The effect of the orientation of oli nucleotide probe and micro forces on DNA hybridization under interface electric field was discussed.
- Electrochemical quartz crystal microbalance( EQCM); DNA hybridization; Interface electric field; False positives
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