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Citation: CHEN Xin, ZHANG Yi-Li, SU Yu-Hua, MENG Wen-Hua, XIE Qing-Ji, YAO Shou-Zhuo. EQCM Studies on Enzymatic Specific Activities of Glucose Oxidase Adsorbed on Au-electroplated and Carbon Nanotubes-modified Au Electrodes[J]. Acta Physico-Chimica Sinica, ;2007, 23(08): 1201-1206. doi: 10.3866/PKU.WHXB20070812 shu

EQCM Studies on Enzymatic Specific Activities of Glucose Oxidase Adsorbed on Au-electroplated and Carbon Nanotubes-modified Au Electrodes

1.

Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China; Medical College, Hunan Normal University, Changsha 410006, P. R. China; Hunan Normal University Hospital, Changsha 410081, P. R. China

Received Date: 5 February 2007
Available Online: 12 June 2007

The electrochemical quartz crystal microbalance (EQCM) technique was employed to monitor in situ the adsorption of glucose oxidase ( D) onto bare Au, Au-electroplated, and carbon nanotubes(CNTs)-modified Au electrodes. The enzymatic specific activities (ESAi) for D adsorbed on various surfaces were measured from the mass of adsorbed D obtained via EQCM characterization and the charge for oxidation of enzymatically generated H2O2. The results showed that D could adsorb on the investigated surfaces with different adsorption amounts, and the adsorbed D was enzymatically active to some degree; the modification of CNTs increased the adsorption amount of D and the response current to glucose, but the ESAi decreased with the increase of the amount of modified CNTs; the Au electroplating on Au electrode also increased the response current to glucose, but the ESAi changed little after the electroplating.
- Electrochemical quartz crystal impedance; Dadsorption; Enzymatic specific activity(ESA); Carbon nanotubes; Au electroplating
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