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Citation: LIU Hui-Hong, WAN Yong-Qing, CHEN Xian-Tang, ZOU Guo-Lin. Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture[J]. Acta Physico-Chimica Sinica, ;2006, 22(07): 868-872. doi: 10.3866/PKU.WHXB20060720 shu

Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture

1.
Department of Chemistry & Biology, Xiangfan University, Xiangfan 441053, P. R. China; College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China

Received Date: 16 January 2006
Available Online: 27 June 2006

Horseradish peroxidase (HRP) was immobilized on glassy carbon electrode (GCE) surface by konjac glucomannan (KGM). HRP entrapped in the KGM film underwent fast direct transfer-electron reactions in water-organic solvent mixtures. The electrocatalysis of peroxides, such as hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and 2-butanone peroxide, by the immobilized HRP was also explored. Stability and reproducibility of the modified electrode made it possible to determine the organic peroxides.
- Horseradish peroxidase;Electrochemistry;Peroxide
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