Home

Citation: MA Guo-Zheng, TAN Fei, JIANG Yong-Jun, ZHENG Ke-Wen, GUO Ming, YU Qing-Sen. The Binding Mode of Pefloxacin Mesylate with Human Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2005, 21(02): 123-127. doi: 10.3866/PKU.WHXB20050203 shu

The Binding Mode of Pefloxacin Mesylate with Human Serum Albumin

1.
Department of Chemistry, Zhejiang University, Hangzhou　310027;　Key Laboratory for Molecular Design and Nutrition Engineering of Ningbo City, Ningbo Institute of Technology, Zhejiang University, Ningbo　315100

Received Date: 8 June 2004
Available Online: 15 February 2005

This study was designed to explore the binding mode of pefloxacin mesylate(PFLX) with human serum albumin (HSA).Fluorescence spectroscopic results showed pefloxacin-HSA complex with binding constants of 1.7×10⁵ L•mol^－1.The enthalpy change (Δ_rH_m) and entropy change (Δ_rS_m) were measured by microcalorimetry to be 1.03 kJ•mol_－1 and 101.28 J•K_－1•mol_－1,which indicated that hydrophobic interaction played a major role in the binding of PFLX with HSA. The possible binding domains of HSA for PFLX were studied by molecular modeling, which predicted that the hydrophobic interaction played a dominating role in this drug-protein interaction and PFLX could bind to Sudlow’s sites I and II of HSA. The binding energies obtained from the molecular modeling were in accordance with the experiment results.
- Pefloxacin mesylate; Human serum albumin; Molecular modeling;　Binding mode
1. [1]
  Yuena Yu , Fang Fang . Microwave-Assisted Synthesis of Safinamide Methanesulfonate. University Chemistry, 2024, 39(11): 210-216. doi: 10.3866/PKU.DXHX202401076
2. [2]
  Yaping Li , Sai An , Aiqing Cao , Shilong Li , Ming Lei . The Application of Molecular Simulation Software in Structural Chemistry Education: First-Principles Calculation of NiFe Layered Double Hydroxide. University Chemistry, 2025, 40(3): 160-170. doi: 10.12461/PKU.DXHX202405185
3. [3]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
4. [4]
  Shuang Meng , Haixin Long , Zhou Zhou , Meizhu Rong . Inorganic Chemistry Curriculum Design and Implementation of Based on “Stepped-Task Driven + Multi-Dimensional Output” Model: A Case Study on Intermolecular Forces. University Chemistry, 2024, 39(3): 122-131. doi: 10.3866/PKU.DXHX202309008
5. [5]
  Jin Yan , Chengxia Tong , Yajie Li , Yue Gu , Xuejian Qu , Shigang Wei , Wanchun Zhu , Yupeng Guo . Construction of a “Dual Support, Triple Integration” Chemical Safety Practical Education System. University Chemistry, 2024, 39(7): 69-75. doi: 10.12461/PKU.DXHX202405008
6. [6]
  Jia Zhou , Huaying Zhong . Experimental Design of Computational Materials Science Combined with Machine Learning. University Chemistry, 2025, 40(3): 171-177. doi: 10.12461/PKU.DXHX202406004
7. [7]
  Yi Li , Zhaoxiang Cao , Peng Liu , Xia Wu , Dongju Zhang . Revealing the Coloration and Color Change Mechanisms of the Eriochrome Black T Indicator through Computational Chemistry and UV-Visible Absorption Spectroscopy. University Chemistry, 2025, 40(3): 132-139. doi: 10.12461/PKU.DXHX202405154
8. [8]
  Jibin Miao , Changjie Mao , Baokang Jin . Exploration and Practice of Virtual and Real Combination Practical Curriculum During the Construction of the National Demonstration Center for Experimental Education: A Case Study of the National Demonstration Center for Experimental Chemistry & Chemical Engineering Education (Anhui University). University Chemistry, 2024, 39(7): 106-109. doi: 10.12461/PKU.DXHX202405021
9. [9]
  Chengxia Tong , Yajie Li , Jin Yan , Xuejian Qu , Shigang Wei , Yong Fan , Zhiguang Song , Yupeng Guo . The Construction and Practice of a Comprehensive and Three-Dimensional Practical Education Model. University Chemistry, 2024, 39(7): 49-55. doi: 10.12461/PKU.DXHX202404155
10. [10]
  Junlin Yan , Changhao Wang , Quanguo Zhai , Chenghui Liu , Dong Xue . A New Construction Model and Practice of Demonstration Center for Experimental Chemistry Education. University Chemistry, 2024, 39(7): 64-68. doi: 10.12461/PKU.DXHX202405005
11. [11]
  Weizhou Jiao , Zhiwei Liu , Chao Zhang , Zhiguo Yuan , Guisheng Qi , Jing Gao . Construction and Implementation of a Mode of Chemical Talent Training Driven by Practice and Innovation Ability. University Chemistry, 2024, 39(7): 76-81. doi: 10.12461/PKU.DXHX202405011
12. [12]
  Yecang Tang , Shan Ling , Zhen Fang . Exploration of a Hierarchical and Integration-Oriented Talent Training Model in the Demonstration Center for Experimental Chemistry Education. University Chemistry, 2024, 39(7): 188-192. doi: 10.12461/PKU.DXHX202405107
13. [13]
  Xu Liu , Chengfang Liu , Jie Huang , Xiangchun Li , Wenyong Lai . Research on the Application of Diversified Teaching Models in the Teaching of Physical Chemistry. University Chemistry, 2024, 39(8): 112-118. doi: 10.3866/PKU.DXHX202402021
14. [14]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Exploration on the Integration Mode of Instrumental Analysis with Science and Education under the Background of Artificial Intelligence Era. University Chemistry, 2024, 39(8): 365-374. doi: 10.12461/PKU.DXHX202403014
15. [15]
  Yan Liu , Xiaojun Han , Ping Xu , Guoxu Zhang , Yu Wang , Zhicheng Zhang , Dianpeng Qi . “Five Measures” Based Science and Education Integration Experimental Teaching Mode to Promote the Construction of “Specialized Experiment” Curriculum. University Chemistry, 2024, 39(10): 299-307. doi: 10.12461/PKU.DXHX202405002
16. [16]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
17. [17]
  Yaqian Duan , Juan Su , Meiyu Lin , Yuxin Fang , Wenyi Liang . Exploration of the Implementation Path of Ideological and Political Education in the “Dual-Track Teaching” Model: a Case Study of Analytical Chemistry Experiment. University Chemistry, 2024, 39(2): 181-188. doi: 10.3866/PKU.DXHX202307024
18. [18]
  Liqiang Lu , Qin Shuai , Xike Tian , Chenggang Zhou , Guo'e Cheng , Bo han , Yulun Nie , Hongtao Zheng , Lei Ouyang . Exploration and Practice of Deep Integration of Production and Education in Applied Chemistry Major under the Background of Emerging Engineering Education. University Chemistry, 2024, 39(3): 138-142. doi: 10.3866/PKU.DXHX202309015
19. [19]
  Yaqing Zhou , Jialin Liu , Jili Wang , Xin Zhou , Yong Sun , Wenhai Wu . Reform and Exploration of Assessment Models for Elective Courses in Local Universities: A Case Study of Hanjiang Normal University. University Chemistry, 2024, 39(4): 244-250. doi: 10.3866/PKU.DXHX202309056
20. [20]
  Xinyue Zhang , Yifeng Ding , Ning Ma . Research on the “Project-based” Master’s Degree Model for Graduate Students in Materials and Chemical Engineering. University Chemistry, 2024, 39(6): 98-102. doi: 10.3866/PKU.DXHX202312093

Get Citation

PDF

XML

Metrics

PDF Downloads(2625)
Abstract views(3447)
HTML views(73)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return