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Citation: Hong Long, Liao Wei, Wei Fang, Zhao Xin-Sheng, Zhu Sheng-Geng. Phage Antibody Chip for Discriminating Proteomes from Different Cells[J]. Acta Physico-Chimica Sinica, ;2004, 20(10): 1182-1185. doi: 10.3866/PKU.WHXB20041003 shu

Phage Antibody Chip for Discriminating Proteomes from Different Cells

1.
School of Life Science, State Key Laboratory of Molecular Dynamic and Stable Structures, and Department of Chemical Biology, College of Chemistry, Peking University, Beijing　100871

Received Date: 7 July 2004
Available Online: 15 October 2004

A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and 1 clone from human to the surface of carboxyl-terminated silicon. The lymphocytes from healthy donors were selected as normal cells, while HeLa cells were selected as tumor cell. The whole proteins were labeled by fluorescent dye Cy3 after isolation from lymphocytes and HeLa cells, and then reacted with the analytical chip. A different binding pattern was obtained. Our results showed that the protein chip made of phage antibodies was able to recognize the proteome from different cells.
- Phage antibody library;Biosensors;Phage antibody chip;Proteome
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