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Citation: Shang Zhi-Cai, Fan Cheng-Ping, Guo Ming, Yu Qing-Sen. A Study on the Interaction between Ofloxacin and Bovine Lactoferrin by Fluorescence Spectrophotometry[J]. Acta Physico-Chimica Sinica, ;2004, 20(08): 864-867. doi: 10.3866/PKU.WHXB20040816 shu

A Study on the Interaction between Ofloxacin and Bovine Lactoferrin by Fluorescence Spectrophotometry

1.
Department of Chemistry, Zhejiang University, Hangzhou　310027

Received Date: 5 January 2004
Available Online: 15 August 2004

The binding reaction between ofloxacin and bovine lactoferrin(BLf) was studied by fluorescence spectra in dilute aqueous solution. The binding constant K was found to be 9.4×104 L•mol－1 and the binding sits n to be 2.43 by fluorescence quenching method. The binding distance (r=2.93 nm) and energy-transfer efficiency (E=0.253) between ofloxacin and bovine lactoferrin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effect of ofloxacin on the conformation of bovine lactoferrin was also analyzed using synchronous fluorescence spectroscopy. The interaction between ofloxacin and bovine lactoferrin is quite strong. Ofloxacin can effect the conformation of bovine lactoferrin in some degree.
- Ofloxacin;Bovine lactoferrin;Fluorescence quenching;Energy transfer
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