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Citation: Wang Mei-Jia, Ji Xiao-Hui, Wang Lian-Ying, Liu Min, Liu Yan-Mei, Bai Yu-Bai, Li Tie-Jin, Li Jing-Hong. The Assembly, Hybridization and Detection of DNA on ld Nanoparticles with Silver Enhancement[J]. Acta Physico-Chimica Sinica, ;2003, 19(09): 879-882. doi: 10.3866/PKU.WHXB20030921 shu

The Assembly, Hybridization and Detection of DNA on ld Nanoparticles with Silver Enhancement

1.
College of Chemistry, Jilin University, Changchun　130023;1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun　130022

Received Date: 17 March 2003
Available Online: 15 September 2003

Authors report on the detection of DNA hybridization using electrochemistry method. The target ss-DNA was immobilized on glassy carbon electrode and hybridized with the ld nanoparticle-oli nucleotide DNA probe. A nanoparticle-promoted Ag precipitation was used to enlarge the nanoparticle tag, forming the Au/Ag core-shell structure, followed by the release of the silver metal atoms anchored on the hybrids by oxidative metal dissolution and the indirect determination of the solubilized Ag ions by anodic stripping voltammetry (ASV) at a carbon fiber microelectrode. Further signal amplification, and lowering of the detection limits to the picomolar domains, was achieved by precipitating silver. The results show that this method has od correlation for detection of DNA in the range of 100～1 000 pmol•L－1and allows detection at levels as low as 10 pmol•L－1 of the target oli nucleotides.
- DNA; ld nanoparticle-oli nucleotide DNA probe;Hybridization;A nanoparticle-promoted Ag precipitation;Anodic stripping voltammetry (ASV)
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