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Citation: Zhu Shi-Guo, Tang Ke, Xiang Juan-Juan, Lü Hong-Bin, Li Xiao-Ling, Nie Xin-Min, Zhou Hou-De, Shen Shou-Rong, Li Gui-Yuan. Synthesis and Applications of Florescence-Doped Silica Nanoparticles[J]. Acta Physico-Chimica Sinica, ;2003, 19(04): 311-314. doi: 10.3866/PKU.WHXB20030406 shu

Synthesis and Applications of Florescence-Doped Silica Nanoparticles

1.
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha　410078

Received Date: 26 August 2002
Available Online: 15 April 2003

The fluorescence-doped silica nanoparticles (FDS-NP) were synthesized in a microemulsion system of polyoxyethylene nonylphenyl ether (OP-10)/ cyclohexane/ammonium hydroxide. To elucidate the character of FDS-NP, techniques of electron microscopy, analysis of spectrum and collisional quenching of fluorescence were used. In order to study the fluorescent signal and intensity of FDS-NP in the cultured cells, laser-scanning confocal microscope and the analysis of flow cytometry were utilized. The results showed that the size of FDS-NP was very small (about 20 nm), the distribution was narrow, the shape was circular and lubricity, and the fluorescence was photostable. Cultured cells could efficiently uptake FDS-NP, and yield high fluorescent signal and intensity. All these indicated that FDS-NP is an important nanoparticulate biomarker, and is applicable to many areas of modern biochemical analysis, such as ultrasensitive detection, immunoassys, as well as cellular biology.
- Nanoparticulate biomarker;Silica nanoparticles;Rhodamine B; Fluorescent nanoparticles;Synthesis and applications
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