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Citation: Zhang Hong-Lin, Yu Xiu-Fang, Zhang Gang. Thermokinetics of Activation of Metal Ions to Amylase Catalyzed Reaction[J]. Acta Physico-Chimica Sinica, ;2002, 18(12): 1125-1128. doi: 10.3866/PKU.WHXB20021214 shu

Thermokinetics of Activation of Metal Ions to Amylase Catalyzed Reaction

1.
Department of Chemistry, Qufu Normal University, Qufu　273165;　1State Key Laboratory of Microbial Technology, Shandong University, Jinan　250100

Received Date: 8 April 2002
Available Online: 15 December 2002

Power－time curves of amylase catalyzed reaction with or without activation were obtained with a 2277 thermal activity monitor(Sweden). The data of Km and vmax were calculated for enzyme-catalyzed reactions without activation. Data of apparent Km1 and vmax were calculated for activation (Ca2＋, Mg2＋) using thermokinetics theory and reduced extent method. From the relationship between and concentration of metal ions(c), the effects of the activation were gained for metal ions of amylase-catalyzed reaction.
- Microcalorimetry;Thermokinetics;Reduced extent;Amylase;Catalyzed reaction
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