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Citation: Sun Bin, Wu Wei, Hong Lian, Zhao Xin-Sheng. Single Crystal Silicon Surface as a Novel Substrate of Gene-chip[J]. Acta Physico-Chimica Sinica, ;2002, 18(07): 581-584. doi: 10.3866/PKU.WHXB20020702 shu

Single Crystal Silicon Surface as a Novel Substrate of Gene-chip

1.
State Key Laboratory of Molecular Dynamic and Stable Structures,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing　100871

Received Date: 18 March 2002
Available Online: 15 July 2002

Single strand oli deoxynucleotide was covalently attached to the alkyl modified silicon (111) surface through ester condensation reaction and the result was characterized by fluorescent spectra.The wafers modified by ss-oli deoxynucleotide were used as DNA probes and the reusability of the resultant wafer was investigated.Its perfect stability in target detection process may promote the wider and more efficient application of the gene chip technology.
- Gene-chip;Silicon(111) surface;Oli deoxynucleotide;Ester condensation reaction;Covalent immobilization;Reusability
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