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Citation: Lai Bing, Li Ying, Cao Ao-Neng, Lai Lu-Hua. Thermodynamic Study on Binding of Archaebacterial RNase HII and Metal Ions[J]. Acta Physico-Chimica Sinica, ;2001, 17(10): 865-867. doi: 10.3866/PKU.WHXB20011001 shu

Thermodynamic Study on Binding of Archaebacterial RNase HII and Metal Ions

1.
State Key Laboratory for Structural Studies of Stable and Unstable Species,Institute of Physical Chemistry,College of Chemistry,Peking University,Beijing　100871

Received Date: 11 June 2001
Available Online: 15 October 2001

RNase H,a ribonuclease specifically degrades the RNA chain in RNA:DNA hybrid,exists vastly in organisms from procaryotic to humanic.We use isothermal titration calorimetry to study the binding thermodynamics of Mg2＋,Mn2＋,Ca2＋ to a type II RNase H from an archaeon:Methanococcus jannaschii.For the first time we obtain the thermodynamic parameters of this binding reaction and verify that these metal ions bind with RNase HII with a ratio of 1:1.This will give an important information for studying RNase HII reaction mechanism and folding properties.
- RNase HII;Isothermal titration calorimetry;Divalent metal ion
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