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Citation: Shang Zhi-Cai, Yi Ping-Gui, Yu Qing-Sen, Lin Rui-Sen. Reaction Mechanism between Ciprofloxacin Hydrochloride and Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2001, 17(01): 48-52. doi: 10.3866/PKU.WHXB20010110 shu

Reaction Mechanism between Ciprofloxacin Hydrochloride and Bovine Serum Albumin

1.
Department of chemistry,Zhejiang University,Hangzhou　310027

Received Date: 15 May 2000
Available Online: 15 January 2001

The reaction between ciprofloxacin and bovine serum albumin(BSA) in aqueous solution was studied by fluorescence spectra and microcalorimetry.The binding constant K was found to be 8.39×104L•mol－1 and the binding sites n to be 1.18 by fluorescence quenching method.The binding distance (r=2.46 nm) and transfer efficiency (E=0.33) between ciprofloxacin and BSA were also obtained according to the Fōrster theory of non-radiation energy transfer.Microcalorimetric measurements showed that the molar change of enthalpy ΔrHm≈0 for the reaction.The interaction between ciprofloxacin and BSA is stronger and the main binding force is hydrophobic interaction.
- Ciprofloxacin;Bovine serum albumin;Fluorescence;Microcalorimetry
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