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Citation: Huang Wen, Li Xiao-Feng, Gu Ti-Ren. Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution[J]. Acta Physico-Chimica Sinica, ;1996, 12(04): 353-356. doi: 10.3866/PKU.WHXB19960413 shu

Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution

1.
Laboratory of Chemistry,Beijing University of Aeronautics and Austronautucs,Beijing 100083

Received Date: 16 June 1995
Available Online: 15 April 1996

The activity and conformation of ribonuclerse A (RNaseA) solubilized in cyclohexane via dodecylammonium butyrate(DAB) reverse micelles were investigated. The activity of RNaseA was studied using the cytidine 2’,3’ -phosphate as the substrate, and it was found that kcat increases significantly with respect to that in water attended by an increased Km•FT-IR spectra of RNaseA in reverse micellar solution were investigated as a function of w0(= [H2O]/ [DAB]), and it was noted that the structure of RNaseA became losser in reverse micelles campared to that in aqueous solution. The relation between activity and conformation was discussed.
- Bovine pancreatic ribonuclease A(RNaseA),
- Dodecylammonium butyrate,
- Reverse micelle,
- FT-IR spectra,
- Surfactant
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