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Bai Chun-Li, Fang Ye, Cao En-Hua, Zhang Ping-Cheng, He Yu-Jian, Tang You-Qi. The Energy Transfer From Triplex dA10·2dT
The Energy Transfer From Triplex dA10·2dT
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The energy transfer from triplex dA_(10)·2dT_(10) or duplex dA_(10)·dA_(10) to ethidium bromide in the 10 mmol·L~(-1) phosphate buffer (pH=7.1) has been investigated. The apparent relative fluorescence quantum yield as a function of excitation wavelength is observed, but the increase in quantum yield in case of the triplex is lower than that for the duplex. This result not only provides strong evidence for intercalative binding of ethidium to both duplex and triplex forms of dA_(10) and dT_(10), but also indicates that binding to the triplex form results in substantially smaller energy transfer than binding to the duplex.
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