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Citation: Zheng Zhi-Min, Wu Hui-Huang, Zhou Shao-Min. Biofunctional Electrodes III. Study on Electrochemical Immobilization of Glucose Oxidase[J]. Acta Physico-Chimica Sinica, ;1991, 7(02): 163-168. doi: 10.3866/PKU.WHXB19910207 shu

Biofunctional Electrodes III. Study on Electrochemical Immobilization of Glucose Oxidase

1.
Department of Chemistry and Institute of Physical Chemistry, Xiamen University, Xiamen 361005

Received Date: 22 December 1989
Available Online: 15 April 1991

By electrochemical polymerization of pyrrole in phosphate buffer, glucose oxidase ( D) was entrapped in polypyrrole matrix (PPy) to construct the enzyme electrode. Effects of electropolymerization conditions, such as pH values and electrode potentials, on the enzyme immobilization were studied. Behavior of D/PPy membranes was characterized by IR and a.c. impedance spectra. The immobilization of D will be accomplished only if pH>5.5. It is likely that D enters into PPy in its entity with negative charge. Spectra of a.c. impedance show that the enzyme electrode has the features similar to porous electrodes. It was edmonstrated that the simultaneous immobilization of D and mediator Fe(CN)_6~(3-) is feasible. The electrcchemically immobilized D maintains its biocatalytic activeties, and apparently the enzymatic reaction follows Michaelis-Menten kinetics.
- Enzyme electrode; Enzyme immobilization; Electropolymerization; Polypyrrole; Glucose oxidase
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