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Citation: Yu Baozhu, Guo Hai, Wu Jinguang. FOURIER TRANSFORM INFRARED STUDY ON THE HYDRATION OF POLYADENYLIC ACID (POLY A) Ⅱ. DYNAMIC HYDRATION AND DEHYDRATION PROPERTIES[J]. Acta Physico-Chimica Sinica, ;1987, 3(06): 642-647. doi: 10.3866/PKU.WHXB19870616 shu

FOURIER TRANSFORM INFRARED STUDY ON THE HYDRATION OF POLYADENYLIC ACID (POLY A) Ⅱ. DYNAMIC HYDRATION AND DEHYDRATION PROPERTIES

1.
Physics Department, Peking University
Chemistry Department, Peking University

Received Date: 1 April 1986
Available Online: 15 December 1987

By taking the N_2 as the carrier gas, the water vapour varying with different relative humidities passed through a sealed sample cell with a thin Poly A film coated on the BaF_2 window to achieve dynamic equilibrium. According to the asending and desending order of relative humidity (from 0% to 100%, then down to 0% again), a series of FT-IR and subtraction spectra have been studied in the region of 4000 to 800 cm~(-1) at (25±1) ℃ (Fig.1, 2). The results are concluded as following:
(1) In comparision with the static process studied by the authors′ previous work~[3], the essential features of hydration effect resemble each other. The strongest hydrating sites are on the —NH_2 and PO_2~- group. But the dynamic hydration behaves faster and stronger than that of static case. During the dynamic process the hydration usually begins at low humidity and then gradually increase; but in the static case, it begins almost around RH=75% then rises steeply (Fig.3). Besides, some new peaks around 1500 cm~(-1) have been observed.
(2) The hydration and dehydration process are irreversible. Both absorbance and peak position can not be restored (Fig.3). It indicates that the hydrating water molecules in Poly A have strong chemical bonding and cannot desorb easily at low humidity. The strongest hysteresis sites are ribose ring —NH_2 and PO_2~- group. So they are not exactly in correspondence with the strongest hydrating sites. This shows, the water molecules may redistribute among the different sites and it takes a quite long time to each equilibrium.
(3) The absorption and desorption isotherm of Poly A film is the well known sigmoidal shape (Fig.5). The number of absorbed water molecules per nucleotide unit at RH=100% is about 8.8. It is roughly approximate to the calculation by Clementi~[4] for the first solvation shell of B-DNA.
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