Citation:
Chun-Lin Sun, Yaole Jiang, Yu Chen, Rongjing Guo, Yongwen Shen, Xinping Hui, Baoxin Zhang, Xiaobo Pan. Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer[J]. University Chemistry,
;2024, 39(5): 287-295.
doi:
10.3866/PKU.DXHX202311096
-
Fluorescence detection has found extensive applications in various fields such as chemical analysis and biological testing. Therefore, conducting experiments related to fluorescence detection is of significant importance in undergraduate laboratory education. This experiment successfully developed a low-cost, compact open-source fluorescence spectrometer, whose performance was comprehensively evaluated and applied to the detection of real samples. The aim is to foster students’ awareness and confidence in designing and creating instruments.
-
-
-
[1]
Lakowicz, J. R. Principles of Fluorescence Spectroscopy, 3rd.; Springer: New York, NY, USA, 2006.
-
[2]
Carstea, E. M.; Bridgeman, J.; Baker, A.; Reynolds, D. M. Water Res. 2016, 95, 205.
-
[3]
Arnaout, R.; Lee, R. A.; Lee, G. R.; Callahan, C.; Yen, C. F.; Smith, K. P.; Arora, R.; Kirby, J. E. bioRxiv 2020, 1.
-
[4]
Vivian, J. T.; Callis, P. R. Biophys. J. 2001, 80, 2093.
-
[5]
Jameson, D. M.; Jameson, I. P. Fluorescence Spectroscopy: New Methods and Applications; CRC Press, Taylor and Francis: New York, NY, USA, 2014.
-
[6]
-
[7]
Sun, C.-L.; Lv, S.-K.; Liu, Y.-P.; Liao, Q.; Zhang, H.-L.; Fu, H.; Yao, J. J. Mater. Chem. C 2017, 5, 1224.
-
[8]
Sun, C. L.; Liao, Q.; Li, T.; Li, J.; Jiang, J. Q.; Xu, Z. Z.; Wang, X. D.; Shen, R.; Bai, D. C.; Wang, Q.; et al. Chem. Sci. 2015, 6, 761.
-
[9]
Sprenger, H. E.; Ziegenbein, W. Angew. Chem. Int. Ed. 2003, 5, 894.
-
[10]
Ros-Lis, J. V.; Garcia, B.; Jimenez, D.; Martinez-Manez, R.; Sancenon, F.; Soto, J.; Gonzalvo, F.; Valldecabres, M. C. J. Am. Chem. Soc. 2004, 126, 4064.
-
[11]
Liu, Y.; Zissis, G.; Li, W.; Chen, D.; Chen, Y.; Buso, D.; Bhosle, S. J. Phys. D Appl. Phys. 2009, 42, 095207.
-
[12]
Tellinghuisen, J. J. Chem. Educ. 1981, 58, 438.
-
[13]
-
[14]
Denk, W.; Strickler, J. H.; Webb, W. W. Science 1990, 248, 73.
-
[1]
-
-
-
[1]
Qin Hou , Jiayi Hou , Aiju Shi , Xingliang Xu , Yuanhong Zhang , Yijing Li , Juying Hou , Yanfang Wang . Preparation of Cuprous Iodide Coordination Polymer and Fluorescent Detection of Nitrite: A Comprehensive Chemical Design Experiment. University Chemistry, 2024, 39(8): 221-229. doi: 10.3866/PKU.DXHX202312056
-
[2]
Rui Gao , Ying Zhou , Yifan Hu , Siyuan Chen , Shouhong Xu , Qianfu Luo , Wenqing Zhang . Design, Synthesis and Performance Experiment of Novel Photoswitchable Hybrid Tetraarylethenes. University Chemistry, 2024, 39(5): 125-133. doi: 10.3866/PKU.DXHX202310050
-
[3]
Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
-
[4]
Naiying Fan , Chuanli Qin , Guo Zhang , Bin Wang , Yan Wang , Bing Zheng , Yichun Qu , Zhiyao Sun , Guanghui An . Case Design of Course Ideological and Political Education in Chemical Experiment Safety: the Safe Use of Common Laboratory Instruments and Glassware. University Chemistry, 2024, 39(2): 242-247. doi: 10.3866/PKU.DXHX202309061
-
[5]
Haiyang Jin , Yonghai Hui , Yongfei Zhang , Lijun Gao , Yun Wang . Application and Exploration of Nuclear Magnetic Resonance Spectrometer in Undergraduate Basic Laboratory Teaching. University Chemistry, 2025, 40(3): 245-250. doi: 10.12461/PKU.DXHX202406022
-
[6]
Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
-
[7]
Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
-
[8]
Xiaofei NIU , Ke WANG , Fengyan SONG , Shuyan YU . Self-assembly of [Pd6(L)4]8+-type macrocyclic complexes for fluorescent sensing of HSO3-. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1233-1242. doi: 10.11862/CJIC.20240057
-
[9]
Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
-
[10]
Xuehua SUN , Min MA , Jianting LIU , Rui TIAN , Hongmei CHAI , Huali CUI , Loujun GAO . Pr/N co-doped biomass carbon dots with enhanced fluorescence for efficient detection of 2,4-dinitrophenylhydrazine. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 561-573. doi: 10.11862/CJIC.20240294
-
[11]
Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching. University Chemistry, 2024, 39(3): 266-273. doi: 10.3866/PKU.DXHX202309068
-
[12]
Xinyu Liu , Weiran Hu , Zhengkai Li , Wei Ji , Xiao Ni . Algin Lab: Surging Luminescent Sea. University Chemistry, 2024, 39(5): 396-404. doi: 10.3866/PKU.DXHX202312021
-
[13]
Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
-
[14]
Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
-
[15]
Lisen Sun , Yongmei Hao , Zhen Huang , Yongmei Liu . Experimental Teaching Design for Viscosity Measurement Serves the Optimization of Operating Conditions for Kitchen Waste Treatment Equipment. University Chemistry, 2024, 39(2): 52-56. doi: 10.3866/PKU.DXHX202307063
-
[16]
Qizhi Yao , Gu Jin , Pingping Zhu . Modular Analytical Chemistry Experimental Teaching Based on “Comprehensive + Exploratory” Experiments: “One Student, One Plan”, Individualized Experimental Teaching Method. University Chemistry, 2024, 39(3): 143-148. doi: 10.3866/PKU.DXHX202309071
-
[17]
Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
-
[18]
Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO3 for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
-
[19]
Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
-
[20]
Kaifu Zhang , Shan Gao , Bin Yang . Application of Theoretical Calculation with Fun Practice in Raman Spectroscopy Experimental Teaching. University Chemistry, 2025, 40(3): 62-67. doi: 10.12461/PKU.DXHX202404045
-
[1]
Metrics
- PDF Downloads(2)
- Abstract views(79)
- HTML views(8)