Citation: Zunxiang Zeng, Yuling Hu, Yufei Hu, Hua Xiao. Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform[J]. University Chemistry, ;2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069 shu

Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform

School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, China

Corresponding author: Zunxiang Zeng, Yuling Hu,
Received Date: 19 September 2023

The analysis of plant essential oil components using gas chromatography-mass spectrometry (GC-MS) serves as an integral component of modern chemical experiment and technology (instrumental analysis part) for undergraduates in our college. The conventional approach of steam distillation for extracting plant essential oil suffers from limitations such as low extraction rate, solvent residue and extended processing time. Supercritical CO₂ extraction, an advanced green extraction technology, offers considerable advantages for the isolation of bioactive constituents from natural plants and animals. In this experiment, citrus essential oil was extracted using supercritical CO₂ extraction and compared with traditional steam distillation method. A total of 106 essential oil components were isolated using supercritical CO₂ extraction, with the following distribution in terms of relative content: terpenes, aldehydes, acids, esters, ketones and alcohols, among which terpenes accounted for 89.55%. The incorporation of supercritical CO₂ extraction technology into the laboratory syllabus not only enriches students’ understanding of advanced sample preparation methods but also enhances their practical skills, research acumen, and innovative thinking. Furthermore, this technology streamlines experiment timeline, reduces the use of hazardous reagents, and promotes green experiment teaching.
- Plant essential oil,
- Supercritical CO₂ extraction,
- Gas chromatography-mass spectrometry,
- Comprehensive experiment
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通讯作者: 陈斌, bchen63@163.com

Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform