Home

Citation: Qin Tu, Anju Tao, Tongtong Ma, Jinyi Wang. Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip[J]. University Chemistry, ;2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062 shu

Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip

Qin Tu ¹ ,
Anju Tao ¹ ,
Tongtong Ma ^1,2 ,
Jinyi Wang ¹

1.
College of Chemistry & Pharmacy, Northwest A & F University, Yangling 712100, Shaanxi Province, China;
2.
Chuxiong Normal University, Chuxiong 675099, Yunnan Province, China

Received Date: 18 September 2023
Revised Date: 10 January 2024

An innovative experimental approach based on a microfluidic paper-based chip has been designed for specific detection of Escherichia coli (E. coli). The chip is fabricated for detecting E. coli in water samples. The main contents include the preparation of paper chips, lysis of E. coli on the chip, detection of E. coli, optimization of detection conditions, and detection of E. coli in actual samples. This experiment integrates cutting-edge disciplines, demonstrating robust comprehensive and innovative characteristics. It aligns with the practical education concept marked by the progression from “basic experiment → comprehensive experiment → research-oriented experiment”, effectively strengthening students’ capacities for problem analysis and solution.
- E. coli,
- Paper-based chip,
- Visual and quantitative detection,
- Innovative experimental
1. [1]
  Shaibani, P.M.; Jiang, K.; Haghighat, G.; Hassanpourfard, M.; Etayash, H.; Naicker, S.;Thundat, T. Sensor. Actuat. B-Chem. 2016, 226, 176.
2. [2]
3. [3]
  Hossain, S.M. Z.; Brennan, J. D. Anal. Chem. 2011, 83(22), 8772.
1. [1]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
2. [2]
  Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
3. [3]
  Yixuan Gao , Lingxing Zan , Wenlin Zhang , Qingbo Wei . Comprehensive Innovation Experiment: Preparation and Characterization of Carbon-based Perovskite Solar Cells. University Chemistry, 2024, 39(4): 178-183. doi: 10.3866/PKU.DXHX202311091
4. [4]
  Shasha Liu , Yongmei Liu , Youqin Li , Juan Wang , Lisen Sun , Jinfen Zhang , Xiang Gao , Xingwen Sun . “Cognitive Experience-Strengthening Foundation-Frontier Innovation”: Construction and Practice of the Chemistry Experimental Curriculum System for Fudan University. University Chemistry, 2024, 39(7): 180-187. doi: 10.12461/PKU.DXHX202405095
5. [5]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
6. [6]
  Yanxin Wang , Hongjuan Wang , Yuren Shi , Yunxia Yang . Application of Python for Visualizing in Structural Chemistry Teaching. University Chemistry, 2024, 39(3): 108-117. doi: 10.3866/PKU.DXHX202306005
7. [7]
  Ruming Yuan , Laiying Zhang , Xiaoming Xu , Pingping Wu , Gang Fu . Application of Mathematica in Visualizing Physical Chemistry Formulas. University Chemistry, 2024, 39(8): 375-382. doi: 10.3866/PKU.DXHX202401030
8. [8]
  Hongyun Liu , Jiarun Li , Xinyi Li , Zhe Liu , Jiaxuan Li , Cong Xiao . Course Ideological and Political Design of a Comprehensive Chemistry Experiment: Constructing a Visual Molecular Logic System Based on Intelligent Hydrogel Film Electrodes. University Chemistry, 2024, 39(2): 227-233. doi: 10.3866/PKU.DXHX202309070
9. [9]
  Dongxue Han , Zhuoyong Li , Hanbo Zou , Xu Wu , Yang Yuan , Hongbin Li . Research on Innovative Experimental Teaching to Cultivate Top Talents. University Chemistry, 2024, 39(7): 230-236. doi: 10.12461/PKU.DXHX202406094
10. [10]
  Qin Li , Kexin Yang , Qinglin Yang , Xiangjin Zhu , Xiaole Han , Tao Huang . Illuminating Chlorophyll: Innovative Chemistry Popularization Experiment. University Chemistry, 2024, 39(9): 359-368. doi: 10.3866/PKU.DXHX202309059
11. [11]
  Qiang Zhou , Pingping Zhu , Wei Shao , Wanqun Hu , Xuan Lei , Haiyang Yang . Innovative Experimental Teaching Design for 3D Printing High-Strength Hydrogel Experiments. University Chemistry, 2024, 39(6): 264-270. doi: 10.3866/PKU.DXHX202310064
12. [12]
  Zhenli Sun , Ning Wang , Kexin Lin , Qin Dai , Yufei Zhou , Dandan Cao , Yanfeng Dang . Visual Analysis of Hotspots and Development Trends in Analytical Chemistry Education Reform. University Chemistry, 2024, 39(11): 57-64. doi: 10.12461/PKU.DXHX202403095
13. [13]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
14. [14]
  Xi Xu , Chaokai Zhu , Leiqing Cao , Zhuozhao Wu , Cao Guan . Experiential Education and 3D-Printed Alloys: Innovative Exploration and Student Development. University Chemistry, 2024, 39(2): 347-357. doi: 10.3866/PKU.DXHX202308039
15. [15]
  Qian Shao , Jiajing Tan , Yongmei Chen , Jiyue Jing , Zhuo Wang . Exploration and Practice on the Management of Extracurricular Innovation Laboratories in Chemistry. University Chemistry, 2024, 39(4): 19-25. doi: 10.3866/PKU.DXHX202310119
16. [16]
  Liangyu Gong , Jie Wang , Fengyu Du , Lubin Xu , Chuanli Ma , Shihai Yan , Zhuwei Song , Fuheng Liu , Xiuzhong Wang . Construction and Practice of “One-Point, Two-Lines and Three-Sides” Innovative Experimental Platform. University Chemistry, 2024, 39(4): 26-32. doi: 10.3866/PKU.DXHX202308023
17. [17]
  Feng Liang , Desheng Li , Yuting Jiang , Jiaxin Dong , Dongcheng Liu , Xingcan Shen . Method Exploration and Instrument Innovation for the Experiment of Colloid ζ Potential Measurement by Electrophoresis. University Chemistry, 2024, 39(5): 345-353. doi: 10.3866/PKU.DXHX202312009
18. [18]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
19. [19]
  Jinglun Wang , Hu Zhou , Baishu Zheng , Guobin Li , Ming Yue , Zhihua Zhou . Exploration and Practice of “Four Cooperations and Four Integrations” to Cultivate Innovative Talents in Chemical Materials in Local Colleges. University Chemistry, 2024, 39(7): 93-98. doi: 10.12461/PKU.DXHX202405013
20. [20]
  Heng Zhang , Gang Liu , Zhenghu Xu , Ying Ma . Construction and Practice of Comprehensive Open and Innovative Chemistry Experimental Teaching System and Platform in the Context of Multidisciplinary Integration. University Chemistry, 2024, 39(7): 56-63. doi: 10.12461/PKU.DXHX202405001

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(355)
HTML views(73)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return