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Citation: Mengting Liu, Shufen Yang, Yu Xue, Jiayan Zang, Quanyan Su, Xingye Zheng, Qiue Cao, Chuanhua Zhou. Preparation of Prussian Blue Analogs Derived Nanoenzymes and Their Enzymatic Catalytic Reaction Kinetics: A Comprehensive Analytical Chemical Experiment[J]. University Chemistry, ;2023, 38(9): 163-171. doi: 10.3866/PKU.DXHX202210012 shu

Preparation of Prussian Blue Analogs Derived Nanoenzymes and Their Enzymatic Catalytic Reaction Kinetics: A Comprehensive Analytical Chemical Experiment

National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical and Technology, Yunnan University, Kunming 650091, China

Corresponding author: Chuanhua Zhou, chzhou@ynu.edu.cn
Received Date: 5 October 2022

An exploratory analytical chemistry comprehensive experiment was designed based on Prussian blue analog-derived nanoenzymes. The experiment includes the preparation and characterization of three kinds of Prussian blue analog-derived nanoenzyme materials and the determination of their enzyme catalytic reaction rate constants. These nanomaterials were further applied to hydrogen peroxide detection. As this experiment features inexpensive reagents, mild reaction conditions, obvious experimental phenomena, and reasonable time allocation, it is suitable for undergraduate laboratory teaching to improve students' experimental skills and cultivate their scientific research and innovation ability.
- Prussian blue nanomaterials,
- Enzyme catalytic reaction kinetics,
- Michaelis constant,
- H₂O₂detection,
- UV-Vis spectrophotometry
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