Citation: Zhongyao Jiang, Yaxin Niu, Nan Wang, Zhenzhen Chen, Bo Tang. Activity-Based Protein Profiling of the Functional Cysteinome[J]. University Chemistry, ;2023, 38(1): 119-128. doi: 10.3866/PKU.DXHX202203011 shu

Activity-Based Protein Profiling of the Functional Cysteinome

Key Laboratory of Molecular and Nano Probes (Ministry of Education), College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, China

Corresponding author: Zhenzhen Chen, zzchen@sdnu.edu.cn
Received Date: 9 March 2022

Proteomic cysteines play important roles in maintaining and regulating redox homeostasis in cells; they also act as functional active site in many proteins and participate in many physiological processes. In addition, post-translationally modifying proteomic cysteines also extends protein functional diversity. The development of chemical proteomics and activity-based protein profiling (ABPP) has led to many important improvements that enable cysteines and their post-translational modifications to be explored. In this paper, we briefly introduce ABPP methods for the proteome-wide profiling of cysteines, present the latest proteomics research into protein-based cysteines and their post-translational modifications, and discuss future developmental trends in this field.
- Cysteine,
- Protein post-translational modifications,
- Activity-based protein profiling,
- Chemical probes
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