Citation: Yuan He, Ye Zhang, Mengchen Wang, Bin Cui, Yan Li, Haiming Fan. Preparation and Kinetic Characterization of Metallo-β-Lactamase L1 in an Online-Offline Blended Teaching Mode[J]. University Chemistry, ;2022, 37(12): 211204. doi: 10.3866/PKU.DXHX202112046 shu

Preparation and Kinetic Characterization of Metallo-β-Lactamase L1 in an Online-Offline Blended Teaching Mode

National Chemistry Experimental Teaching Demonstration Center, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China

Received Date: 17 December 2021

Recombinant protein preparation is an important part of many chemical biology experiments, but its effective implementation in a regular undergraduate experiment course is limited by the long period of time required. This work reports innovative improvements on the content and teaching methods in an undergraduate teaching experiment: “Preparation and kinetic characterization of metallo-β-lactamase L1”. The main improvements include: (1) using online virtual simulation experiments on a mobile device to study the whole process of L1 preparation and conduct a kinetics study; and (2) offline classroom operation for compulsory and extended experiments. The online resource contains 10 modules, such as polymerase chain reaction of a target gene, recombinant plasmid transformation, protein induction, etc. High-quality simulation of experimental scenarios and human-machine interactive operations provide students with a highly immersive experience, and the coherent experimental arrangement presents a clear knowledge framework. A new offline extension experiment was added to enable the use of the isothermal titration microcalorimetric method to characterize the kinetic parameters of the L1 enzyme. This blended teaching mode realizes the integration of online and offline teaching, which can not only improve the quality and efficiency of teaching, but also broaden students’ horizons and cultivate their hands-on ability and innovation awareness. Thus far, good feedback has been received from preliminary implementation, which is expected to provide a reference for undergraduate experimental teaching in other institutions.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
  Majiduddin, F. K.; Materon, I. C.; Palzkill, T. G. Int. J. Med. Microbiol. 2002, 292 (2), 127.
7. [7]
  O’Neill, J. Antimicrobial Resistance: Tackling a crisis for the health and wealth of nations. [2022-03-25]. https://amr-review.org/
8. [8]
  Tooke, C. L.; Hinchliffe, P.; Bragginton, E. C.; Colenso, C. K.; Hirvonen, V. H. A.; Takebayashi, Y.; Spencer, J. J. Mol. Biol. 2019, 431 (18), 3472.
9. [9]
  Coulthurst, S. J.; Barnard, A. M.; Salmond, G. P. Nat. Rev. Microbiol. 2005, 3 (4), 295.
10. [10]
  Wang, W. J.; Wang, Q.; Zhang, Y.; Lu, R.; Zhang, Y. L.; Yang, K. W.; Lei, J. E.; He, Y. Biochim. Biophys. Acta Gen. Subj. 2017, 1861 (8), 2031.
11. [11]
  Zhang, Y.; Lei, J. E.; He, Y.; Yang, J.; Wang, W.; Wasey, A.; Xu, J.; Lin, Y.; Fan, H.; Jing, G.; et al. Angew. Chem. Int. Ed. 2018, 57 (52), 17120.
12. [12]
  Todd, M. J.; Gomez, J. Anal. Biochem. 2001, 296 (2), 179.
1. [1]
  Zhiguang Xu , Xuan Xu , Qiong Luo , Ganquan Wang , Bin Peng . Reform and Practice of Online and Offline Blended Teaching in Structural Chemistry Course. University Chemistry, 2024, 39(6): 195-200. doi: 10.3866/PKU.DXHX202310112
2. [2]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
3. [3]
  Shengyan Yang , Xiangzhen Meng , Xin Wang , Yang Zhang . Construction and Exploration of an Online-Offline Blended “Eight-Link” Teaching Method for Physical Chemistry Experiments Based on OBE Concept. University Chemistry, 2024, 39(11): 28-37. doi: 10.3866/PKU.DXHX202402019
4. [4]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044
5. [5]
  Xiaojun Wu , Kai Hu , Faqiong Zhao . Laying the Groundwork for General Chemistry Experiment Teaching: Exploration and Summary of Assisting Experiment Preparatory Work through Online and Offline Integration. University Chemistry, 2024, 39(8): 23-27. doi: 10.3866/PKU.DXHX202312052
6. [6]
  Dexin Tan , Limin Liang , Baoyi Lv , Huiwen Guan , Haicheng Chen , Yanli Wang . Exploring Reverse Teaching Practices in Physical Chemistry Experiment Courses: A Case Study on Chemical Reaction Kinetics. University Chemistry, 2024, 39(11): 79-86. doi: 10.12461/PKU.DXHX202403048
7. [7]
  Shule Liu . Application of SPC/E Water Model in Molecular Dynamics Teaching Experiments. University Chemistry, 2024, 39(4): 338-342. doi: 10.3866/PKU.DXHX202310029
8. [8]
  Yulian Hu , Xin Zhou , Xiaojun Han . A Virtual Simulation Experiment on the Design and Property Analysis of CO₂ Reduction Photocatalyst. University Chemistry, 2025, 40(3): 30-35. doi: 10.12461/PKU.DXHX202403088
9. [9]
  Xuzhen Wang , Xinkui Wang , Dongxu Tian , Wei Liu . Enhancing the Comprehensive Quality and Innovation Abilities of Graduate Students through a “Student-Centered, Dual Integration and Dual Drive” Teaching Model: A Case Study in the Course of Chemical Reaction Kinetics. University Chemistry, 2024, 39(6): 160-165. doi: 10.3866/PKU.DXHX202401074
10. [10]
  Yiying Yang , Dongju Zhang . Elucidating the Concepts of Thermodynamic Control and Kinetic Control in Chemical Reactions through Theoretical Chemistry Calculations: A Computational Chemistry Experiment on the Diels-Alder Reaction. University Chemistry, 2024, 39(3): 327-335. doi: 10.3866/PKU.DXHX202309074
11. [11]
  Jinfu Ma , Hui Lu , Jiandong Wu , Zhongli Zou . Teaching Design of Electrochemical Principles Course Based on “Cognitive Laws”: Kinetics of Electron Transfer Steps. University Chemistry, 2024, 39(3): 174-177. doi: 10.3866/PKU.DXHX202309052
12. [12]
  Pingping Zhu , Yongjun Xie , Yuanping Yi , Yu Huang , Qiang Zhou , Shiyan Xiao , Haiyang Yang , Pingsheng He . Excavation and Extraction of Ideological and Political Elements for the Virtual Simulation Experiments at Molecular Level: Taking the Project “the Simulation and Computation of Conformation, Morphology and Dimensions of Polymer Chains” as an Example. University Chemistry, 2024, 39(2): 83-88. doi: 10.3866/PKU.DXHX202309063
13. [13]
  Shanghua Li , Malin Li , Xiwen Chi , Xin Yin , Zhaodi Luo , Jihong Yu . 基于高离子迁移动力学的取向ZnQ分子筛保护层实现高稳定水系锌金属负极的构筑. Acta Physico-Chimica Sinica, 2025, 41(1): 2309003-. doi: 10.3866/PKU.WHXB202309003
14. [14]
  Hui Xiong , Yan Wang , Rongxian Bai , Yongqi Wu , Chengmei Liu , Yuefa Gong , Jian Zhang . Development of a Compound Talent Training System Based on Virtual Technology: a Case Study of Chemical Unit and Process Simulation Practices. University Chemistry, 2024, 39(10): 314-317. doi: 10.12461/PKU.DXHX202405071
15. [15]
  Yue Wu , Jun Li , Bo Zhang , Yan Yang , Haibo Li , Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028
16. [16]
  Hui Shi , Shuangyan Huan , Yuzhi Wang . Ideological and Political Design of Potassium Permanganate Oxidation-Reduction Titration Experiment. University Chemistry, 2024, 39(2): 175-180. doi: 10.3866/PKU.DXHX202308042
17. [17]
  Yan Li , Xinze Wang , Xue Yao , Shouyun Yu . 基于激发态手性铜催化的烯烃E→Z异构的动力学拆分——推荐一个本科生综合化学实验. University Chemistry, 2024, 39(5): 1-10. doi: 10.3866/PKU.DXHX202309053
18. [18]
  Yang Chen , Xiuying Wang , Nengqin Jia . Ideological and Political Design, Blended Teaching Practice of Physical Chemistry Experiment: Pb-Sn Binary Metal Phase Diagram. University Chemistry, 2025, 40(3): 223-229. doi: 10.12461/PKU.DXHX202405184
19. [19]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
20. [20]
  Yang Lv , Yingping Jia , Yanhua Li , Hexiang Zhong , Xinping Wang . Integrating the Ideological Elements with the “Chemical Reaction Heat” Teaching. University Chemistry, 2024, 39(11): 44-51. doi: 10.12461/PKU.DXHX202402059

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(567)
HTML views(88)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142