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Citation: Ruiqing Sun, Ziting Xu, Zhuling Chen, Jiali Lin. Experimental Design on the Determination of Dissociation Constants of Eriochrome Black T by Spectrophotometry[J]. University Chemistry, ;2022, 37(12): 211203. doi: 10.3866/PKU.DXHX202112039 shu

Experimental Design on the Determination of Dissociation Constants of Eriochrome Black T by Spectrophotometry

National Experimental Teaching Demonstration Center for Chemistry and Chemical Engineering, College of Chemistry, Fuzhou University, Fuzhou 350116, China

Corresponding author: Ruiqing Sun, 1031598981@qq.com
Received Date: 14 December 2021

This work aims to provide an approach to determine the dissociation constant (pK_a) of Eriochrome Black T by using UV-Visible spectrophotometry. The absorbance of Eriochrome Black T was measured at different pH and the data was fitted by ORIGIN with specific function model. Based on the obtained formula, the three pK_a of Eriochrome Black T can be calculated, respectively. This method is highly efficient, accurate, and easy to operate. It can also serve as an expansion for the undergraduates’ chemistry laboratory, which can help cultivate the students’ comprehensive ability of experimental design, data processing, and model derivation.
- Dissociation constant,
- Ultraviolet-visible spectrophotometry,
- Eriochrome Black T
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