Citation: Zhengli Hu, Kaili Xin, Shaochuang Liu, Chengbing Zhong, Xueyuan Wu, Yi-Lun Ying, Xuanfeng Kong, Xiaodong Yu, Jianrong Zhang, Yi-Tao Long. 生物纳米孔道单分子多肽磷酸化识别与测量的创新综合实验[J]. University Chemistry, ;2022, 37(7): 211108. doi: 10.3866/PKU.DXHX202111089 shu

生物纳米孔道单分子多肽磷酸化识别与测量的创新综合实验

1.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
2.
Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210023, China;
3.
Shenzhen Research Institute of Nanjing University, Shenzhen 518057, Guangdong Province, China

Corresponding author: Yi-Tao Long, yitaolong@nju.edu.cn
Received Date: 30 November 2021

生物纳米孔道技术是一种具有革命性意义的单分子电化学分析方法，经过近三十年的发展，已经能够实现单分子DNA测序并广泛应用于多肽、蛋白质等单个生物分子的检测研究。本文探讨了将纳米孔道单分子测量方法引入大学实验教学的必要性与重要性，介绍了生物纳米孔道单分子实验的教学意义、目的、内容及其组织实施方式。本实验采用“科教融合”的教学模式，针对具有一定化学、生物化学基础知识并对科研感兴趣的二、三年级本科生，精心设计并不断优化教学方案，将纳米孔道单分子电化学及相关交叉领域的最新研究成果融入课堂教学，拓展学生的科研视野、激发学生的科研兴趣。此外，通过教师引导，鼓励学生大胆提出问题，自主探索，合作完成实验内容，培养学生的学术思维和综合创新能力。
- Biological nanopore,
- Single-molecule measurement,
- Peptide phosphorylation,
- Integration of science and education,
- Comprehensive chemistry experiment
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