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Citation: Yumeng Zhang, Yuyan Chen, Yan Liu. 电喷雾多级质谱研究功能小分子与蛋白质的非共价相互作用——推荐一个化学生物学教学实验[J]. University Chemistry, ;2021, 36(12): 210206. doi: 10.3866/PKU.DXHX202102062 shu

电喷雾多级质谱研究功能小分子与蛋白质的非共价相互作用——推荐一个化学生物学教学实验

Fujian Provincial Key Laboratory of Chemical Biology (Xiamen University), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China

Corresponding author: Yan Liu, stacyliu@xmu.edu.cn
Received Date: 28 February 2021

肌红蛋白不仅便宜易得，而且自身就是一种由蛋白质和铁卟啉环辅基通过非共价相互作用构成的复合物。因此，以肌红蛋白为研究模型，带领学生实践基于电喷雾多级质谱技术的酶抑制剂直接筛选策略，重点考查不同有机溶剂比例及pH等样品制备条件对复合物测定结果的影响，以及如何利用二级质谱技术实现功能小分子配体的在线定性分析。该实验已在我校化学生物学专业本科生“化学生物学综合实验”教学工作中进行了多轮教学实践，通过实践不断优化实验设计，取得了良好的教学效果。
- Electrospray multistage mass spectrometry,
- Non-covalent interaction,
- Myoglobin
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